Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03897 and H281DRAFT_03898 are separated by 12 nucleotides H281DRAFT_03898 and H281DRAFT_03899 overlap by 4 nucleotides H281DRAFT_03899 and H281DRAFT_03900 are separated by 22 nucleotides
H281DRAFT_03897: H281DRAFT_03897 - hypothetical protein, at 429,132 to 433,940
_03897
H281DRAFT_03898: H281DRAFT_03898 - DUF2300 domain-containing protein YfaQ, at 433,953 to 435,737
_03898
H281DRAFT_03899: H281DRAFT_03899 - Uncharacterized conserved protein YfaP, DUF2135 family, at 435,734 to 436,618
_03899
H281DRAFT_03900: H281DRAFT_03900 - Uncharacterized conserved protein YfaP, DUF2135 family, at 436,641 to 437,441
_03900
Position (kb) 433
434
435
436 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 433.084 kb on + strand, within H281DRAFT_03897 at 433.456 kb on + strand, within H281DRAFT_03897 at 433.564 kb on - strand at 433.564 kb on - strand at 433.564 kb on - strand at 433.564 kb on - strand at 433.611 kb on + strand at 433.743 kb on + strand at 433.744 kb on - strand at 433.860 kb on + strand at 433.872 kb on + strand at 433.872 kb on + strand at 433.873 kb on - strand at 433.873 kb on - strand at 434.067 kb on + strand at 434.068 kb on - strand at 434.259 kb on + strand, within H281DRAFT_03898 at 434.298 kb on + strand, within H281DRAFT_03898 at 434.298 kb on + strand, within H281DRAFT_03898 at 434.299 kb on - strand, within H281DRAFT_03898 at 434.331 kb on + strand, within H281DRAFT_03898 at 434.331 kb on + strand, within H281DRAFT_03898 at 434.332 kb on - strand, within H281DRAFT_03898 at 434.332 kb on - strand, within H281DRAFT_03898 at 434.628 kb on + strand, within H281DRAFT_03898 at 435.127 kb on - strand, within H281DRAFT_03898 at 435.585 kb on + strand at 435.586 kb on - strand at 435.586 kb on - strand at 435.591 kb on + strand at 435.592 kb on - strand at 435.636 kb on + strand at 435.636 kb on + strand at 435.637 kb on - strand at 435.637 kb on - strand at 435.637 kb on - strand at 435.637 kb on - strand at 436.095 kb on - strand, within H281DRAFT_03899 at 436.203 kb on - strand, within H281DRAFT_03899 at 436.203 kb on - strand, within H281DRAFT_03899 at 436.415 kb on - strand, within H281DRAFT_03899 at 436.415 kb on - strand, within H281DRAFT_03899 at 436.437 kb on - strand, within H281DRAFT_03899 at 436.534 kb on + strand at 436.681 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 433,084 + H281DRAFT_03897 0.82 +0.0 433,456 + H281DRAFT_03897 0.90 -0.4 433,564 - -0.1 433,564 - +1.1 433,564 - -0.4 433,564 - +0.0 433,611 + +0.2 433,743 + +2.0 433,744 - -0.7 433,860 + +0.8 433,872 + +4.3 433,872 + -0.2 433,873 - +0.3 433,873 - +0.3 434,067 + +0.4 434,068 - +0.6 434,259 + H281DRAFT_03898 0.17 -0.2 434,298 + H281DRAFT_03898 0.19 -1.3 434,298 + H281DRAFT_03898 0.19 -0.5 434,299 - H281DRAFT_03898 0.19 -0.0 434,331 + H281DRAFT_03898 0.21 -0.8 434,331 + H281DRAFT_03898 0.21 +0.3 434,332 - H281DRAFT_03898 0.21 +0.9 434,332 - H281DRAFT_03898 0.21 +0.3 434,628 + H281DRAFT_03898 0.38 -0.5 435,127 - H281DRAFT_03898 0.66 +0.8 435,585 + -0.2 435,586 - +0.3 435,586 - -0.6 435,591 + +1.0 435,592 - +0.5 435,636 + +0.6 435,636 + -0.2 435,637 - +0.9 435,637 - +0.2 435,637 - +1.3 435,637 - +0.3 436,095 - H281DRAFT_03899 0.41 +0.1 436,203 - H281DRAFT_03899 0.53 +0.4 436,203 - H281DRAFT_03899 0.53 -0.1 436,415 - H281DRAFT_03899 0.77 +0.5 436,415 - H281DRAFT_03899 0.77 +0.0 436,437 - H281DRAFT_03899 0.79 -0.5 436,534 + +0.7 436,681 - +0.6
Or see this region's nucleotide sequence