Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03608 and H281DRAFT_03609 are separated by 22 nucleotides H281DRAFT_03609 and H281DRAFT_03610 overlap by 4 nucleotides
H281DRAFT_03608: H281DRAFT_03608 - farnesyl-diphosphate farnesyltransferase, at 116,800 to 117,648
_03608
H281DRAFT_03609: H281DRAFT_03609 - squalene-associated FAD-dependent desaturase, at 117,671 to 118,921
_03609
H281DRAFT_03610: H281DRAFT_03610 - squalene-hopene/tetraprenyl-beta-curcumene cyclase, at 118,918 to 120,978
_03610
Position (kb) 117
118
119 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 116.807 kb on + strand at 117.340 kb on + strand, within H281DRAFT_03608 at 117.666 kb on + strand at 117.666 kb on + strand at 117.666 kb on + strand at 117.666 kb on + strand at 117.666 kb on + strand at 117.666 kb on + strand at 117.667 kb on - strand at 117.667 kb on - strand at 117.785 kb on + strand at 117.860 kb on + strand, within H281DRAFT_03609 at 117.871 kb on + strand, within H281DRAFT_03609 at 118.031 kb on + strand, within H281DRAFT_03609 at 118.610 kb on + strand, within H281DRAFT_03609 at 118.802 kb on - strand at 118.888 kb on + strand at 118.929 kb on - strand at 119.182 kb on + strand, within H281DRAFT_03610 at 119.314 kb on + strand, within H281DRAFT_03610 at 119.470 kb on - strand at 119.571 kb on - strand at 119.860 kb on + strand, within H281DRAFT_03610 at 119.861 kb on - strand, within H281DRAFT_03610
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 116,807 + -0.7 117,340 + H281DRAFT_03608 0.64 -0.2 117,666 + +0.3 117,666 + -0.5 117,666 + -0.6 117,666 + -0.1 117,666 + +0.9 117,666 + -0.3 117,667 - +1.0 117,667 - -2.2 117,785 + -0.9 117,860 + H281DRAFT_03609 0.15 -0.4 117,871 + H281DRAFT_03609 0.16 +0.1 118,031 + H281DRAFT_03609 0.29 +0.4 118,610 + H281DRAFT_03609 0.75 -0.6 118,802 - -1.0 118,888 + +0.6 118,929 - -0.6 119,182 + H281DRAFT_03610 0.13 -3.3 119,314 + H281DRAFT_03610 0.19 -2.3 119,470 - +1.0 119,571 - -0.0 119,860 + H281DRAFT_03610 0.46 +0.6 119,861 - H281DRAFT_03610 0.46 -3.1
Or see this region's nucleotide sequence