Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03550 and H281DRAFT_03551 are separated by 291 nucleotides H281DRAFT_03551 and H281DRAFT_03552 are separated by 267 nucleotides
H281DRAFT_03550: H281DRAFT_03550 - two-component system, NarL family, capsular synthesis sensor histidine kinase RcsC, at 51,087 to 54,605
_03550
H281DRAFT_03551: H281DRAFT_03551 - methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor, at 54,897 to 56,603
_03551
H281DRAFT_03552: H281DRAFT_03552 - Uncharacterized damage-inducible protein DinB (forms a four-helix bundle), at 56,871 to 57,473
_03552
Position (kb)
54
55
56
57 Strain fitness (log2 ratio)
-1
0
1
2 at 54.158 kb on - strand, within H281DRAFT_03550 at 54.328 kb on + strand at 54.331 kb on + strand at 54.332 kb on - strand at 54.332 kb on - strand at 54.332 kb on - strand at 54.335 kb on - strand at 54.518 kb on - strand at 54.518 kb on - strand at 54.688 kb on + strand at 54.689 kb on - strand at 54.800 kb on - strand at 54.838 kb on - strand at 55.026 kb on - strand at 55.284 kb on + strand, within H281DRAFT_03551 at 55.698 kb on + strand, within H281DRAFT_03551 at 55.698 kb on + strand, within H281DRAFT_03551 at 55.779 kb on - strand, within H281DRAFT_03551 at 56.272 kb on + strand, within H281DRAFT_03551 at 56.521 kb on + strand at 56.521 kb on + strand at 56.521 kb on + strand at 56.521 kb on + strand at 56.521 kb on + strand at 56.522 kb on - strand at 56.522 kb on - strand at 56.522 kb on - strand at 56.642 kb on + strand at 56.653 kb on + strand at 56.653 kb on + strand at 56.653 kb on + strand at 56.654 kb on - strand at 56.659 kb on + strand at 56.659 kb on + strand at 56.660 kb on - strand at 56.687 kb on - strand at 56.716 kb on + strand at 56.716 kb on + strand at 56.716 kb on + strand at 56.716 kb on + strand at 56.716 kb on + strand at 56.716 kb on + strand at 56.717 kb on - strand at 56.717 kb on - strand at 56.717 kb on - strand at 56.793 kb on + strand at 56.793 kb on + strand at 56.794 kb on - strand at 56.794 kb on - strand at 57.075 kb on + strand, within H281DRAFT_03552 at 57.076 kb on - strand, within H281DRAFT_03552 at 57.078 kb on + strand, within H281DRAFT_03552 at 57.078 kb on + strand, within H281DRAFT_03552 at 57.078 kb on + strand, within H281DRAFT_03552 at 57.079 kb on - strand, within H281DRAFT_03552 at 57.472 kb on - strand at 57.472 kb on - strand at 57.498 kb on - strand at 57.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 54,158 - H281DRAFT_03550 0.87 +0.6 54,328 + +0.8 54,331 + +0.2 54,332 - +1.0 54,332 - -0.2 54,332 - +0.0 54,335 - -0.2 54,518 - -1.4 54,518 - +1.1 54,688 + +0.7 54,689 - -0.4 54,800 - -1.0 54,838 - -0.5 55,026 - -0.4 55,284 + H281DRAFT_03551 0.23 -0.4 55,698 + H281DRAFT_03551 0.47 +0.3 55,698 + H281DRAFT_03551 0.47 -1.1 55,779 - H281DRAFT_03551 0.52 -0.4 56,272 + H281DRAFT_03551 0.81 -0.6 56,521 + -0.5 56,521 + +0.8 56,521 + -0.9 56,521 + +0.2 56,521 + -0.8 56,522 - -0.2 56,522 - -0.5 56,522 - +0.5 56,642 + -1.1 56,653 + -0.3 56,653 + -0.6 56,653 + -0.4 56,654 - -0.2 56,659 + -0.2 56,659 + -1.5 56,660 - -0.6 56,687 - +0.7 56,716 + +0.3 56,716 + +0.2 56,716 + +0.2 56,716 + +0.5 56,716 + +1.8 56,716 + -0.5 56,717 - +0.2 56,717 - -0.5 56,717 - -0.8 56,793 + +1.1 56,793 + -0.6 56,794 - +0.4 56,794 - -1.7 57,075 + H281DRAFT_03552 0.34 -0.2 57,076 - H281DRAFT_03552 0.34 -0.8 57,078 + H281DRAFT_03552 0.34 -0.3 57,078 + H281DRAFT_03552 0.34 +0.5 57,078 + H281DRAFT_03552 0.34 -1.3 57,079 - H281DRAFT_03552 0.34 -0.2 57,472 - -0.1 57,472 - -1.4 57,498 - +0.3 57,518 + -0.1
Or see this region's nucleotide sequence