Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03362 and H281DRAFT_03363 are separated by 12 nucleotides H281DRAFT_03363 and H281DRAFT_03364 overlap by 8 nucleotides
H281DRAFT_03362: H281DRAFT_03362 - Acyl-CoA dehydrogenase, at 421,632 to 422,813
_03362
H281DRAFT_03363: H281DRAFT_03363 - fumarate reductase (CoM/CoB) subunit A, at 422,826 to 424,604
_03363
H281DRAFT_03364: H281DRAFT_03364 - Crotonobetainyl-CoA:carnitine CoA-transferase CaiB, at 424,597 to 425,820
_03364
Position (kb) 422
423
424
425 Strain fitness (log2 ratio) -1
0
1
2 at 421.989 kb on - strand, within H281DRAFT_03362 at 422.119 kb on + strand, within H281DRAFT_03362 at 422.120 kb on - strand, within H281DRAFT_03362 at 422.166 kb on + strand, within H281DRAFT_03362 at 422.166 kb on + strand, within H281DRAFT_03362 at 422.167 kb on - strand, within H281DRAFT_03362 at 422.169 kb on - strand, within H281DRAFT_03362 at 422.238 kb on - strand, within H281DRAFT_03362 at 422.313 kb on + strand, within H281DRAFT_03362 at 422.464 kb on - strand, within H281DRAFT_03362 at 422.635 kb on - strand, within H281DRAFT_03362 at 422.648 kb on + strand, within H281DRAFT_03362 at 422.648 kb on + strand, within H281DRAFT_03362 at 422.648 kb on + strand, within H281DRAFT_03362 at 422.652 kb on + strand, within H281DRAFT_03362 at 422.653 kb on - strand, within H281DRAFT_03362 at 423.076 kb on - strand, within H281DRAFT_03363 at 423.237 kb on + strand, within H281DRAFT_03363 at 423.237 kb on + strand, within H281DRAFT_03363 at 423.314 kb on - strand, within H281DRAFT_03363 at 423.362 kb on + strand, within H281DRAFT_03363 at 423.363 kb on - strand, within H281DRAFT_03363 at 423.363 kb on - strand at 423.592 kb on - strand, within H281DRAFT_03363 at 423.672 kb on + strand, within H281DRAFT_03363 at 424.211 kb on + strand, within H281DRAFT_03363 at 424.212 kb on - strand, within H281DRAFT_03363 at 424.412 kb on + strand, within H281DRAFT_03363 at 424.636 kb on - strand at 425.005 kb on + strand, within H281DRAFT_03364 at 425.162 kb on + strand, within H281DRAFT_03364 at 425.199 kb on + strand, within H281DRAFT_03364 at 425.320 kb on + strand, within H281DRAFT_03364 at 425.320 kb on + strand, within H281DRAFT_03364 at 425.342 kb on - strand, within H281DRAFT_03364 at 425.512 kb on + strand, within H281DRAFT_03364 at 425.567 kb on - strand, within H281DRAFT_03364
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 421,989 - H281DRAFT_03362 0.30 +0.3 422,119 + H281DRAFT_03362 0.41 -0.3 422,120 - H281DRAFT_03362 0.41 -0.2 422,166 + H281DRAFT_03362 0.45 -0.5 422,166 + H281DRAFT_03362 0.45 +0.2 422,167 - H281DRAFT_03362 0.45 -0.5 422,169 - H281DRAFT_03362 0.45 -0.9 422,238 - H281DRAFT_03362 0.51 -0.3 422,313 + H281DRAFT_03362 0.58 +0.9 422,464 - H281DRAFT_03362 0.70 -0.1 422,635 - H281DRAFT_03362 0.85 +0.1 422,648 + H281DRAFT_03362 0.86 -0.2 422,648 + H281DRAFT_03362 0.86 -0.1 422,648 + H281DRAFT_03362 0.86 -0.5 422,652 + H281DRAFT_03362 0.86 +0.0 422,653 - H281DRAFT_03362 0.86 -0.4 423,076 - H281DRAFT_03363 0.14 +0.6 423,237 + H281DRAFT_03363 0.23 -0.8 423,237 + H281DRAFT_03363 0.23 +0.7 423,314 - H281DRAFT_03363 0.27 +0.8 423,362 + H281DRAFT_03363 0.30 -0.3 423,363 - H281DRAFT_03363 0.30 -1.2 423,363 - +2.4 423,592 - H281DRAFT_03363 0.43 +0.2 423,672 + H281DRAFT_03363 0.48 +0.7 424,211 + H281DRAFT_03363 0.78 -0.3 424,212 - H281DRAFT_03363 0.78 +0.1 424,412 + H281DRAFT_03363 0.89 +0.0 424,636 - -0.2 425,005 + H281DRAFT_03364 0.33 +0.2 425,162 + H281DRAFT_03364 0.46 -0.0 425,199 + H281DRAFT_03364 0.49 -0.4 425,320 + H281DRAFT_03364 0.59 +0.1 425,320 + H281DRAFT_03364 0.59 -0.2 425,342 - H281DRAFT_03364 0.61 -0.4 425,512 + H281DRAFT_03364 0.75 +0.6 425,567 - H281DRAFT_03364 0.79 -0.5
Or see this region's nucleotide sequence