Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03360 and H281DRAFT_03361 are separated by 12 nucleotides H281DRAFT_03361 and H281DRAFT_03362 are separated by 20 nucleotides H281DRAFT_03362 and H281DRAFT_03363 are separated by 12 nucleotides
H281DRAFT_03360: H281DRAFT_03360 - succinate-semialdehyde dehydrogenase / glutarate-semialdehyde dehydrogenase, at 419,293 to 420,774
_03360
H281DRAFT_03361: H281DRAFT_03361 - 2-keto-3-deoxy-L-rhamnonate aldolase RhmA, at 420,787 to 421,611
_03361
H281DRAFT_03362: H281DRAFT_03362 - Acyl-CoA dehydrogenase, at 421,632 to 422,813
_03362
H281DRAFT_03363: H281DRAFT_03363 - fumarate reductase (CoM/CoB) subunit A, at 422,826 to 424,604
_03363
Position (kb) 421
422
423 Strain fitness (log2 ratio) -1
0
1
2 at 420.709 kb on + strand at 421.087 kb on - strand, within H281DRAFT_03361 at 421.116 kb on + strand, within H281DRAFT_03361 at 421.116 kb on + strand, within H281DRAFT_03361 at 421.117 kb on - strand, within H281DRAFT_03361 at 421.365 kb on + strand, within H281DRAFT_03361 at 421.365 kb on + strand, within H281DRAFT_03361 at 421.444 kb on + strand, within H281DRAFT_03361 at 421.468 kb on + strand, within H281DRAFT_03361 at 421.676 kb on + strand at 421.684 kb on - strand at 421.755 kb on + strand, within H281DRAFT_03362 at 421.755 kb on + strand, within H281DRAFT_03362 at 421.756 kb on - strand, within H281DRAFT_03362 at 421.989 kb on - strand, within H281DRAFT_03362 at 422.119 kb on + strand, within H281DRAFT_03362 at 422.120 kb on - strand, within H281DRAFT_03362 at 422.166 kb on + strand, within H281DRAFT_03362 at 422.166 kb on + strand, within H281DRAFT_03362 at 422.167 kb on - strand, within H281DRAFT_03362 at 422.169 kb on - strand, within H281DRAFT_03362 at 422.238 kb on - strand, within H281DRAFT_03362 at 422.313 kb on + strand, within H281DRAFT_03362 at 422.464 kb on - strand, within H281DRAFT_03362 at 422.635 kb on - strand, within H281DRAFT_03362 at 422.648 kb on + strand, within H281DRAFT_03362 at 422.648 kb on + strand, within H281DRAFT_03362 at 422.648 kb on + strand, within H281DRAFT_03362 at 422.652 kb on + strand, within H281DRAFT_03362 at 422.653 kb on - strand, within H281DRAFT_03362 at 423.076 kb on - strand, within H281DRAFT_03363 at 423.237 kb on + strand, within H281DRAFT_03363 at 423.237 kb on + strand, within H281DRAFT_03363 at 423.314 kb on - strand, within H281DRAFT_03363 at 423.362 kb on + strand, within H281DRAFT_03363 at 423.363 kb on - strand, within H281DRAFT_03363 at 423.363 kb on - strand at 423.592 kb on - strand, within H281DRAFT_03363 at 423.672 kb on + strand, within H281DRAFT_03363
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 420,709 + -0.8 421,087 - H281DRAFT_03361 0.36 -0.2 421,116 + H281DRAFT_03361 0.40 +0.0 421,116 + H281DRAFT_03361 0.40 +0.5 421,117 - H281DRAFT_03361 0.40 -1.1 421,365 + H281DRAFT_03361 0.70 +0.1 421,365 + H281DRAFT_03361 0.70 +0.8 421,444 + H281DRAFT_03361 0.80 +0.6 421,468 + H281DRAFT_03361 0.83 -0.8 421,676 + -0.5 421,684 - +2.0 421,755 + H281DRAFT_03362 0.10 +0.4 421,755 + H281DRAFT_03362 0.10 -0.6 421,756 - H281DRAFT_03362 0.10 +0.3 421,989 - H281DRAFT_03362 0.30 +0.3 422,119 + H281DRAFT_03362 0.41 -0.3 422,120 - H281DRAFT_03362 0.41 -0.2 422,166 + H281DRAFT_03362 0.45 -0.5 422,166 + H281DRAFT_03362 0.45 +0.2 422,167 - H281DRAFT_03362 0.45 -0.5 422,169 - H281DRAFT_03362 0.45 -0.9 422,238 - H281DRAFT_03362 0.51 -0.3 422,313 + H281DRAFT_03362 0.58 +0.9 422,464 - H281DRAFT_03362 0.70 -0.1 422,635 - H281DRAFT_03362 0.85 +0.1 422,648 + H281DRAFT_03362 0.86 -0.2 422,648 + H281DRAFT_03362 0.86 -0.1 422,648 + H281DRAFT_03362 0.86 -0.5 422,652 + H281DRAFT_03362 0.86 +0.0 422,653 - H281DRAFT_03362 0.86 -0.4 423,076 - H281DRAFT_03363 0.14 +0.6 423,237 + H281DRAFT_03363 0.23 -0.8 423,237 + H281DRAFT_03363 0.23 +0.7 423,314 - H281DRAFT_03363 0.27 +0.8 423,362 + H281DRAFT_03363 0.30 -0.3 423,363 - H281DRAFT_03363 0.30 -1.2 423,363 - +2.4 423,592 - H281DRAFT_03363 0.43 +0.2 423,672 + H281DRAFT_03363 0.48 +0.7
Or see this region's nucleotide sequence