Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_02566

Experiment: LB in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_02564 and H281DRAFT_02565 are separated by 33 nucleotidesH281DRAFT_02565 and H281DRAFT_02566 are separated by 52 nucleotidesH281DRAFT_02566 and H281DRAFT_02567 are separated by 148 nucleotides H281DRAFT_02564: H281DRAFT_02564 - amino acid ABC transporter substrate-binding protein, PAAT family, at 139,265 to 140,077 _02564 H281DRAFT_02565: H281DRAFT_02565 - amino acid ABC transporter ATP-binding protein, PAAT family, at 140,111 to 140,836 _02565 H281DRAFT_02566: H281DRAFT_02566 - Glycine/D-amino acid oxidase (deaminating), at 140,889 to 142,214 _02566 H281DRAFT_02567: H281DRAFT_02567 - DNA-binding transcriptional regulator, LysR family, at 142,363 to 143,265 _02567 Position (kb) 140 141 142 143Strain fitness (log2 ratio) -2 -1 0 1at 139.932 kb on + strand, within H281DRAFT_02564at 139.933 kb on - strand, within H281DRAFT_02564at 140.440 kb on - strand, within H281DRAFT_02565at 140.924 kb on + strandat 141.329 kb on - strand, within H281DRAFT_02566at 141.474 kb on - strand, within H281DRAFT_02566at 142.859 kb on + strand, within H281DRAFT_02567

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL
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139,932 + H281DRAFT_02564 0.82 -0.5
139,933 - H281DRAFT_02564 0.82 +1.3
140,440 - H281DRAFT_02565 0.45 +0.1
140,924 + -2.0
141,329 - H281DRAFT_02566 0.33 +0.4
141,474 - H281DRAFT_02566 0.44 +1.2
142,859 + H281DRAFT_02567 0.55 -0.2

Or see this region's nucleotide sequence