Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02425 and H281DRAFT_02426 are separated by 23 nucleotides H281DRAFT_02426 and H281DRAFT_02427 overlap by 4 nucleotides H281DRAFT_02427 and H281DRAFT_02428 overlap by 4 nucleotides H281DRAFT_02428 and H281DRAFT_02429 are separated by 17 nucleotides
H281DRAFT_02425: H281DRAFT_02425 - NAD-dependent aldehyde dehydrogenases, at 597,421 to 598,638
_02425
H281DRAFT_02426: H281DRAFT_02426 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, at 598,662 to 599,135
_02426
H281DRAFT_02427: H281DRAFT_02427 - Choline dehydrogenase, at 599,132 to 600,736
_02427
H281DRAFT_02428: H281DRAFT_02428 - hypothetical protein, at 600,733 to 601,155
_02428
H281DRAFT_02429: H281DRAFT_02429 - 3-oxoacyl-[acyl-carrier protein] reductase, at 601,173 to 601,916
_02429
Position (kb) 599
600
601 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6 at 598.164 kb on - strand, within H281DRAFT_02425 at 598.240 kb on - strand, within H281DRAFT_02425 at 598.270 kb on + strand, within H281DRAFT_02425 at 598.342 kb on + strand, within H281DRAFT_02425 at 598.463 kb on - strand, within H281DRAFT_02425 at 598.572 kb on + strand at 598.573 kb on - strand at 598.579 kb on - strand at 598.583 kb on + strand at 598.626 kb on - strand at 598.701 kb on + strand at 598.726 kb on + strand, within H281DRAFT_02426 at 598.727 kb on - strand, within H281DRAFT_02426 at 598.764 kb on + strand, within H281DRAFT_02426 at 598.764 kb on + strand, within H281DRAFT_02426 at 598.776 kb on + strand, within H281DRAFT_02426 at 598.779 kb on - strand, within H281DRAFT_02426 at 598.779 kb on - strand, within H281DRAFT_02426 at 598.803 kb on + strand, within H281DRAFT_02426 at 598.835 kb on + strand, within H281DRAFT_02426 at 598.942 kb on + strand at 598.942 kb on + strand, within H281DRAFT_02426 at 598.943 kb on - strand, within H281DRAFT_02426 at 598.943 kb on - strand, within H281DRAFT_02426 at 598.943 kb on - strand, within H281DRAFT_02426 at 598.943 kb on - strand, within H281DRAFT_02426 at 599.106 kb on + strand at 599.106 kb on + strand at 599.106 kb on + strand at 599.107 kb on - strand at 599.107 kb on - strand at 599.131 kb on + strand at 599.131 kb on + strand at 599.310 kb on - strand, within H281DRAFT_02427 at 599.311 kb on + strand, within H281DRAFT_02427 at 599.311 kb on + strand, within H281DRAFT_02427 at 599.312 kb on - strand, within H281DRAFT_02427 at 599.312 kb on - strand, within H281DRAFT_02427 at 599.374 kb on + strand, within H281DRAFT_02427 at 599.374 kb on + strand, within H281DRAFT_02427 at 599.374 kb on + strand, within H281DRAFT_02427 at 599.375 kb on - strand, within H281DRAFT_02427 at 599.375 kb on - strand, within H281DRAFT_02427 at 599.375 kb on - strand, within H281DRAFT_02427 at 599.377 kb on - strand, within H281DRAFT_02427 at 599.377 kb on - strand, within H281DRAFT_02427 at 599.610 kb on + strand, within H281DRAFT_02427 at 599.696 kb on - strand, within H281DRAFT_02427 at 599.746 kb on + strand, within H281DRAFT_02427 at 599.789 kb on + strand, within H281DRAFT_02427 at 599.789 kb on + strand, within H281DRAFT_02427 at 599.790 kb on - strand, within H281DRAFT_02427 at 599.888 kb on + strand at 599.888 kb on + strand, within H281DRAFT_02427 at 599.888 kb on + strand at 599.888 kb on + strand, within H281DRAFT_02427 at 599.889 kb on - strand, within H281DRAFT_02427 at 599.889 kb on - strand, within H281DRAFT_02427 at 599.890 kb on + strand, within H281DRAFT_02427 at 600.062 kb on + strand, within H281DRAFT_02427 at 600.063 kb on - strand, within H281DRAFT_02427 at 600.140 kb on + strand, within H281DRAFT_02427 at 600.140 kb on + strand, within H281DRAFT_02427 at 600.176 kb on + strand, within H281DRAFT_02427 at 600.316 kb on + strand, within H281DRAFT_02427 at 600.631 kb on + strand at 600.632 kb on - strand at 600.854 kb on + strand, within H281DRAFT_02428 at 600.971 kb on + strand, within H281DRAFT_02428 at 600.971 kb on + strand, within H281DRAFT_02428 at 600.971 kb on + strand, within H281DRAFT_02428 at 600.972 kb on - strand, within H281DRAFT_02428 at 600.972 kb on - strand, within H281DRAFT_02428 at 600.972 kb on - strand, within H281DRAFT_02428 at 600.972 kb on - strand, within H281DRAFT_02428 at 600.972 kb on - strand, within H281DRAFT_02428 at 601.012 kb on + strand, within H281DRAFT_02428 at 601.121 kb on - strand at 601.180 kb on + strand at 601.181 kb on - strand at 601.432 kb on + strand, within H281DRAFT_02429 at 601.433 kb on - strand, within H281DRAFT_02429
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 598,164 - H281DRAFT_02425 0.61 +4.3 598,240 - H281DRAFT_02425 0.67 +4.9 598,270 + H281DRAFT_02425 0.70 +4.8 598,342 + H281DRAFT_02425 0.76 +5.1 598,463 - H281DRAFT_02425 0.86 +4.6 598,572 + +5.5 598,573 - +4.8 598,579 - +4.8 598,583 + +4.4 598,626 - +4.9 598,701 + -0.2 598,726 + H281DRAFT_02426 0.14 +0.3 598,727 - H281DRAFT_02426 0.14 +4.0 598,764 + H281DRAFT_02426 0.22 +0.6 598,764 + H281DRAFT_02426 0.22 -0.7 598,776 + H281DRAFT_02426 0.24 +0.2 598,779 - H281DRAFT_02426 0.25 +4.1 598,779 - H281DRAFT_02426 0.25 +3.6 598,803 + H281DRAFT_02426 0.30 -0.7 598,835 + H281DRAFT_02426 0.36 +0.3 598,942 + +0.6 598,942 + H281DRAFT_02426 0.59 -0.1 598,943 - H281DRAFT_02426 0.59 +4.0 598,943 - H281DRAFT_02426 0.59 +3.6 598,943 - H281DRAFT_02426 0.59 +3.5 598,943 - H281DRAFT_02426 0.59 +4.3 599,106 + -0.1 599,106 + -0.3 599,106 + -0.2 599,107 - +4.1 599,107 - +5.0 599,131 + +1.6 599,131 + -0.5 599,310 - H281DRAFT_02427 0.11 +3.4 599,311 + H281DRAFT_02427 0.11 +4.1 599,311 + H281DRAFT_02427 0.11 +3.3 599,312 - H281DRAFT_02427 0.11 +2.0 599,312 - H281DRAFT_02427 0.11 +3.2 599,374 + H281DRAFT_02427 0.15 +3.4 599,374 + H281DRAFT_02427 0.15 +5.7 599,374 + H281DRAFT_02427 0.15 +3.1 599,375 - H281DRAFT_02427 0.15 +3.9 599,375 - H281DRAFT_02427 0.15 +2.8 599,375 - H281DRAFT_02427 0.15 +3.6 599,377 - H281DRAFT_02427 0.15 +4.1 599,377 - H281DRAFT_02427 0.15 +4.7 599,610 + H281DRAFT_02427 0.30 +5.0 599,696 - H281DRAFT_02427 0.35 +3.4 599,746 + H281DRAFT_02427 0.38 +2.7 599,789 + H281DRAFT_02427 0.41 +3.6 599,789 + H281DRAFT_02427 0.41 +4.0 599,790 - H281DRAFT_02427 0.41 +3.7 599,888 + +4.0 599,888 + H281DRAFT_02427 0.47 +3.8 599,888 + +5.0 599,888 + H281DRAFT_02427 0.47 +3.8 599,889 - H281DRAFT_02427 0.47 +3.8 599,889 - H281DRAFT_02427 0.47 +3.6 599,890 + H281DRAFT_02427 0.47 +3.6 600,062 + H281DRAFT_02427 0.58 +3.2 600,063 - H281DRAFT_02427 0.58 +3.3 600,140 + H281DRAFT_02427 0.63 +4.9 600,140 + H281DRAFT_02427 0.63 +3.6 600,176 + H281DRAFT_02427 0.65 +3.7 600,316 + H281DRAFT_02427 0.74 +4.2 600,631 + +4.4 600,632 - +1.3 600,854 + H281DRAFT_02428 0.29 +6.3 600,971 + H281DRAFT_02428 0.56 +4.1 600,971 + H281DRAFT_02428 0.56 +3.3 600,971 + H281DRAFT_02428 0.56 +3.5 600,972 - H281DRAFT_02428 0.57 +4.4 600,972 - H281DRAFT_02428 0.57 +5.2 600,972 - H281DRAFT_02428 0.57 +3.5 600,972 - H281DRAFT_02428 0.57 +4.1 600,972 - H281DRAFT_02428 0.57 +4.0 601,012 + H281DRAFT_02428 0.66 +4.0 601,121 - +3.9 601,180 + +4.6 601,181 - +3.7 601,432 + H281DRAFT_02429 0.35 +5.4 601,433 - H281DRAFT_02429 0.35 +4.2
Or see this region's nucleotide sequence