Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02425 and H281DRAFT_02426 are separated by 23 nucleotides H281DRAFT_02426 and H281DRAFT_02427 overlap by 4 nucleotides
H281DRAFT_02425: H281DRAFT_02425 - NAD-dependent aldehyde dehydrogenases, at 597,421 to 598,638
_02425
H281DRAFT_02426: H281DRAFT_02426 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, at 598,662 to 599,135
_02426
H281DRAFT_02427: H281DRAFT_02427 - Choline dehydrogenase, at 599,132 to 600,736
_02427
Position (kb) 598
599
600 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 597.869 kb on + strand, within H281DRAFT_02425 at 597.869 kb on + strand, within H281DRAFT_02425 at 597.870 kb on - strand, within H281DRAFT_02425 at 597.969 kb on + strand, within H281DRAFT_02425 at 597.969 kb on + strand, within H281DRAFT_02425 at 597.970 kb on - strand, within H281DRAFT_02425 at 597.970 kb on - strand, within H281DRAFT_02425 at 598.114 kb on + strand, within H281DRAFT_02425 at 598.114 kb on + strand, within H281DRAFT_02425 at 598.115 kb on - strand, within H281DRAFT_02425 at 598.115 kb on - strand, within H281DRAFT_02425 at 598.164 kb on - strand, within H281DRAFT_02425 at 598.240 kb on - strand, within H281DRAFT_02425 at 598.270 kb on + strand, within H281DRAFT_02425 at 598.342 kb on + strand, within H281DRAFT_02425 at 598.463 kb on - strand, within H281DRAFT_02425 at 598.572 kb on + strand at 598.573 kb on - strand at 598.579 kb on - strand at 598.583 kb on + strand at 598.626 kb on - strand at 598.701 kb on + strand at 598.726 kb on + strand, within H281DRAFT_02426 at 598.727 kb on - strand, within H281DRAFT_02426 at 598.764 kb on + strand, within H281DRAFT_02426 at 598.764 kb on + strand, within H281DRAFT_02426 at 598.776 kb on + strand, within H281DRAFT_02426 at 598.779 kb on - strand, within H281DRAFT_02426 at 598.779 kb on - strand, within H281DRAFT_02426 at 598.803 kb on + strand, within H281DRAFT_02426 at 598.835 kb on + strand, within H281DRAFT_02426 at 598.942 kb on + strand at 598.942 kb on + strand, within H281DRAFT_02426 at 598.943 kb on - strand, within H281DRAFT_02426 at 598.943 kb on - strand, within H281DRAFT_02426 at 598.943 kb on - strand, within H281DRAFT_02426 at 598.943 kb on - strand, within H281DRAFT_02426 at 599.106 kb on + strand at 599.106 kb on + strand at 599.106 kb on + strand at 599.107 kb on - strand at 599.107 kb on - strand at 599.131 kb on + strand at 599.131 kb on + strand at 599.310 kb on - strand, within H281DRAFT_02427 at 599.311 kb on + strand, within H281DRAFT_02427 at 599.311 kb on + strand, within H281DRAFT_02427 at 599.312 kb on - strand, within H281DRAFT_02427 at 599.312 kb on - strand, within H281DRAFT_02427 at 599.374 kb on + strand, within H281DRAFT_02427 at 599.374 kb on + strand, within H281DRAFT_02427 at 599.374 kb on + strand, within H281DRAFT_02427 at 599.375 kb on - strand, within H281DRAFT_02427 at 599.375 kb on - strand, within H281DRAFT_02427 at 599.375 kb on - strand, within H281DRAFT_02427 at 599.377 kb on - strand, within H281DRAFT_02427 at 599.377 kb on - strand, within H281DRAFT_02427 at 599.610 kb on + strand, within H281DRAFT_02427 at 599.696 kb on - strand, within H281DRAFT_02427 at 599.746 kb on + strand, within H281DRAFT_02427 at 599.789 kb on + strand, within H281DRAFT_02427 at 599.789 kb on + strand, within H281DRAFT_02427 at 599.790 kb on - strand, within H281DRAFT_02427 at 599.888 kb on + strand at 599.888 kb on + strand, within H281DRAFT_02427 at 599.888 kb on + strand at 599.888 kb on + strand, within H281DRAFT_02427 at 599.889 kb on - strand, within H281DRAFT_02427 at 599.889 kb on - strand, within H281DRAFT_02427 at 599.890 kb on + strand, within H281DRAFT_02427 at 600.062 kb on + strand, within H281DRAFT_02427 at 600.063 kb on - strand, within H281DRAFT_02427
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 597,869 + H281DRAFT_02425 0.37 +5.5 597,869 + H281DRAFT_02425 0.37 +5.3 597,870 - H281DRAFT_02425 0.37 +5.2 597,969 + H281DRAFT_02425 0.45 +4.7 597,969 + H281DRAFT_02425 0.45 +4.2 597,970 - H281DRAFT_02425 0.45 +3.5 597,970 - H281DRAFT_02425 0.45 +4.3 598,114 + H281DRAFT_02425 0.57 +4.0 598,114 + H281DRAFT_02425 0.57 +4.5 598,115 - H281DRAFT_02425 0.57 +4.3 598,115 - H281DRAFT_02425 0.57 +5.2 598,164 - H281DRAFT_02425 0.61 +4.3 598,240 - H281DRAFT_02425 0.67 +4.9 598,270 + H281DRAFT_02425 0.70 +4.8 598,342 + H281DRAFT_02425 0.76 +5.1 598,463 - H281DRAFT_02425 0.86 +4.6 598,572 + +5.5 598,573 - +4.8 598,579 - +4.8 598,583 + +4.4 598,626 - +4.9 598,701 + -0.2 598,726 + H281DRAFT_02426 0.14 +0.3 598,727 - H281DRAFT_02426 0.14 +4.0 598,764 + H281DRAFT_02426 0.22 +0.6 598,764 + H281DRAFT_02426 0.22 -0.7 598,776 + H281DRAFT_02426 0.24 +0.2 598,779 - H281DRAFT_02426 0.25 +4.1 598,779 - H281DRAFT_02426 0.25 +3.6 598,803 + H281DRAFT_02426 0.30 -0.7 598,835 + H281DRAFT_02426 0.36 +0.3 598,942 + +0.6 598,942 + H281DRAFT_02426 0.59 -0.1 598,943 - H281DRAFT_02426 0.59 +4.0 598,943 - H281DRAFT_02426 0.59 +3.6 598,943 - H281DRAFT_02426 0.59 +3.5 598,943 - H281DRAFT_02426 0.59 +4.3 599,106 + -0.1 599,106 + -0.3 599,106 + -0.2 599,107 - +4.1 599,107 - +5.0 599,131 + +1.6 599,131 + -0.5 599,310 - H281DRAFT_02427 0.11 +3.4 599,311 + H281DRAFT_02427 0.11 +4.1 599,311 + H281DRAFT_02427 0.11 +3.3 599,312 - H281DRAFT_02427 0.11 +2.0 599,312 - H281DRAFT_02427 0.11 +3.2 599,374 + H281DRAFT_02427 0.15 +3.4 599,374 + H281DRAFT_02427 0.15 +5.7 599,374 + H281DRAFT_02427 0.15 +3.1 599,375 - H281DRAFT_02427 0.15 +3.9 599,375 - H281DRAFT_02427 0.15 +2.8 599,375 - H281DRAFT_02427 0.15 +3.6 599,377 - H281DRAFT_02427 0.15 +4.1 599,377 - H281DRAFT_02427 0.15 +4.7 599,610 + H281DRAFT_02427 0.30 +5.0 599,696 - H281DRAFT_02427 0.35 +3.4 599,746 + H281DRAFT_02427 0.38 +2.7 599,789 + H281DRAFT_02427 0.41 +3.6 599,789 + H281DRAFT_02427 0.41 +4.0 599,790 - H281DRAFT_02427 0.41 +3.7 599,888 + +4.0 599,888 + H281DRAFT_02427 0.47 +3.8 599,888 + +5.0 599,888 + H281DRAFT_02427 0.47 +3.8 599,889 - H281DRAFT_02427 0.47 +3.8 599,889 - H281DRAFT_02427 0.47 +3.6 599,890 + H281DRAFT_02427 0.47 +3.6 600,062 + H281DRAFT_02427 0.58 +3.2 600,063 - H281DRAFT_02427 0.58 +3.3
Or see this region's nucleotide sequence