Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01895 and H281DRAFT_01896 are separated by 94 nucleotides H281DRAFT_01896 and H281DRAFT_01897 overlap by 31 nucleotides
H281DRAFT_01895: H281DRAFT_01895 - drug resistance transporter, EmrB/QacA subfamily, at 7,555 to 9,195
_01895
H281DRAFT_01896: H281DRAFT_01896 - EcsC protein family protein, at 9,290 to 10,132
_01896
H281DRAFT_01897: H281DRAFT_01897 - Acetyl esterase/lipase, at 10,102 to 11,064
_01897
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1 at 8.304 kb on + strand, within H281DRAFT_01895 at 8.304 kb on + strand, within H281DRAFT_01895 at 8.304 kb on + strand, within H281DRAFT_01895 at 8.304 kb on + strand, within H281DRAFT_01895 at 8.305 kb on - strand, within H281DRAFT_01895 at 8.308 kb on + strand, within H281DRAFT_01895 at 8.390 kb on + strand, within H281DRAFT_01895 at 8.609 kb on - strand, within H281DRAFT_01895 at 8.612 kb on + strand, within H281DRAFT_01895 at 8.634 kb on - strand, within H281DRAFT_01895 at 8.636 kb on + strand, within H281DRAFT_01895 at 8.637 kb on - strand, within H281DRAFT_01895 at 8.751 kb on + strand, within H281DRAFT_01895 at 8.815 kb on + strand, within H281DRAFT_01895 at 8.904 kb on - strand, within H281DRAFT_01895 at 8.906 kb on + strand, within H281DRAFT_01895 at 8.907 kb on - strand, within H281DRAFT_01895 at 8.957 kb on + strand, within H281DRAFT_01895 at 8.957 kb on + strand, within H281DRAFT_01895 at 8.957 kb on + strand, within H281DRAFT_01895 at 8.957 kb on + strand, within H281DRAFT_01895 at 8.958 kb on - strand, within H281DRAFT_01895 at 8.979 kb on - strand, within H281DRAFT_01895 at 8.979 kb on - strand, within H281DRAFT_01895 at 8.980 kb on + strand, within H281DRAFT_01895 at 8.980 kb on + strand, within H281DRAFT_01895 at 8.980 kb on + strand, within H281DRAFT_01895 at 8.980 kb on + strand, within H281DRAFT_01895 at 8.981 kb on - strand, within H281DRAFT_01895 at 8.981 kb on - strand, within H281DRAFT_01895 at 8.981 kb on - strand, within H281DRAFT_01895 at 9.013 kb on - strand, within H281DRAFT_01895 at 9.195 kb on + strand at 9.195 kb on + strand at 9.195 kb on + strand at 9.195 kb on + strand at 9.214 kb on - strand at 9.269 kb on + strand at 9.269 kb on + strand at 9.269 kb on + strand at 9.270 kb on - strand at 9.270 kb on - strand at 9.595 kb on + strand, within H281DRAFT_01896 at 9.596 kb on - strand, within H281DRAFT_01896 at 9.689 kb on - strand, within H281DRAFT_01896 at 9.800 kb on + strand, within H281DRAFT_01896 at 9.800 kb on + strand, within H281DRAFT_01896 at 10.391 kb on + strand, within H281DRAFT_01897 at 10.392 kb on - strand, within H281DRAFT_01897 at 10.410 kb on + strand, within H281DRAFT_01897 at 10.411 kb on - strand, within H281DRAFT_01897 at 10.411 kb on - strand, within H281DRAFT_01897 at 10.440 kb on + strand, within H281DRAFT_01897 at 10.658 kb on + strand, within H281DRAFT_01897 at 10.659 kb on - strand, within H281DRAFT_01897 at 10.721 kb on + strand, within H281DRAFT_01897 at 10.802 kb on + strand, within H281DRAFT_01897 at 10.802 kb on + strand, within H281DRAFT_01897 at 10.802 kb on + strand, within H281DRAFT_01897 at 10.803 kb on - strand, within H281DRAFT_01897 at 10.823 kb on + strand, within H281DRAFT_01897 at 10.823 kb on + strand, within H281DRAFT_01897 at 10.824 kb on - strand, within H281DRAFT_01897 at 10.824 kb on - strand, within H281DRAFT_01897 at 10.824 kb on - strand, within H281DRAFT_01897 at 10.824 kb on - strand, within H281DRAFT_01897 at 11.099 kb on + strand at 11.099 kb on + strand at 11.099 kb on + strand at 11.099 kb on + strand at 11.100 kb on - strand at 11.100 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 8,304 + H281DRAFT_01895 0.46 +0.3 8,304 + H281DRAFT_01895 0.46 +0.1 8,304 + H281DRAFT_01895 0.46 -0.0 8,304 + H281DRAFT_01895 0.46 -0.3 8,305 - H281DRAFT_01895 0.46 -0.1 8,308 + H281DRAFT_01895 0.46 +0.3 8,390 + H281DRAFT_01895 0.51 -0.3 8,609 - H281DRAFT_01895 0.64 -0.6 8,612 + H281DRAFT_01895 0.64 +0.1 8,634 - H281DRAFT_01895 0.66 +0.0 8,636 + H281DRAFT_01895 0.66 +1.0 8,637 - H281DRAFT_01895 0.66 -1.5 8,751 + H281DRAFT_01895 0.73 -0.2 8,815 + H281DRAFT_01895 0.77 +0.3 8,904 - H281DRAFT_01895 0.82 -0.8 8,906 + H281DRAFT_01895 0.82 -0.2 8,907 - H281DRAFT_01895 0.82 -0.6 8,957 + H281DRAFT_01895 0.85 +0.1 8,957 + H281DRAFT_01895 0.85 -0.2 8,957 + H281DRAFT_01895 0.85 +1.4 8,957 + H281DRAFT_01895 0.85 +0.3 8,958 - H281DRAFT_01895 0.85 +0.4 8,979 - H281DRAFT_01895 0.87 +0.1 8,979 - H281DRAFT_01895 0.87 -0.3 8,980 + H281DRAFT_01895 0.87 +0.4 8,980 + H281DRAFT_01895 0.87 -0.5 8,980 + H281DRAFT_01895 0.87 -0.4 8,980 + H281DRAFT_01895 0.87 +0.3 8,981 - H281DRAFT_01895 0.87 +0.1 8,981 - H281DRAFT_01895 0.87 +0.3 8,981 - H281DRAFT_01895 0.87 -0.0 9,013 - H281DRAFT_01895 0.89 +1.2 9,195 + +1.5 9,195 + -0.3 9,195 + +0.1 9,195 + +0.3 9,214 - +0.3 9,269 + +0.6 9,269 + -1.9 9,269 + +1.2 9,270 - +0.5 9,270 - -0.7 9,595 + H281DRAFT_01896 0.36 +0.2 9,596 - H281DRAFT_01896 0.36 +1.7 9,689 - H281DRAFT_01896 0.47 +1.2 9,800 + H281DRAFT_01896 0.60 +0.8 9,800 + H281DRAFT_01896 0.60 -0.0 10,391 + H281DRAFT_01897 0.30 -1.2 10,392 - H281DRAFT_01897 0.30 +1.0 10,410 + H281DRAFT_01897 0.32 +0.3 10,411 - H281DRAFT_01897 0.32 -0.1 10,411 - H281DRAFT_01897 0.32 +1.0 10,440 + H281DRAFT_01897 0.35 +0.1 10,658 + H281DRAFT_01897 0.58 -0.8 10,659 - H281DRAFT_01897 0.58 -1.2 10,721 + H281DRAFT_01897 0.64 -0.6 10,802 + H281DRAFT_01897 0.73 +0.1 10,802 + H281DRAFT_01897 0.73 -0.4 10,802 + H281DRAFT_01897 0.73 -0.5 10,803 - H281DRAFT_01897 0.73 +0.2 10,823 + H281DRAFT_01897 0.75 +0.1 10,823 + H281DRAFT_01897 0.75 -0.3 10,824 - H281DRAFT_01897 0.75 +1.2 10,824 - H281DRAFT_01897 0.75 -0.6 10,824 - H281DRAFT_01897 0.75 -0.7 10,824 - H281DRAFT_01897 0.75 +0.4 11,099 + -0.2 11,099 + +1.3 11,099 + +0.4 11,099 + -0.7 11,100 - +0.7 11,100 - +0.1
Or see this region's nucleotide sequence