Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00815 and H281DRAFT_00816 are separated by 297 nucleotides H281DRAFT_00816 and H281DRAFT_00817 are separated by 131 nucleotides H281DRAFT_00817 and H281DRAFT_00818 are separated by 216 nucleotides H281DRAFT_00818 and H281DRAFT_00819 are separated by 210 nucleotides
H281DRAFT_00815: H281DRAFT_00815 - hemoglobin, at 903,894 to 904,436
_00815
H281DRAFT_00816: H281DRAFT_00816 - transcriptional regulator, BadM/Rrf2 family, at 904,734 to 905,243
_00816
H281DRAFT_00817: H281DRAFT_00817 - ATP-dependent Clp protease ATP-binding subunit ClpB, at 905,375 to 907,972
_00817
H281DRAFT_00818: H281DRAFT_00818 - Uncharacterized protein conserved in bacteria, at 908,189 to 908,617
_00818
H281DRAFT_00819: H281DRAFT_00819 - competence protein ComEA, at 908,828 to 909,439
_00819
Position (kb) 905
906
907
908 Strain fitness (log2 ratio) -2
-1
0
1
2 at 904.625 kb on + strand at 904.629 kb on + strand at 904.724 kb on + strand at 904.725 kb on - strand at 904.725 kb on - strand at 904.733 kb on + strand at 904.734 kb on - strand at 904.734 kb on - strand at 904.734 kb on - strand at 904.734 kb on - strand at 904.749 kb on + strand at 904.750 kb on - strand at 904.750 kb on - strand at 904.777 kb on + strand at 904.780 kb on - strand at 904.780 kb on - strand at 904.822 kb on + strand, within H281DRAFT_00816 at 904.822 kb on + strand, within H281DRAFT_00816 at 904.823 kb on - strand, within H281DRAFT_00816 at 904.833 kb on + strand, within H281DRAFT_00816 at 905.170 kb on + strand, within H281DRAFT_00816 at 905.171 kb on - strand, within H281DRAFT_00816 at 905.171 kb on - strand, within H281DRAFT_00816 at 905.456 kb on + strand at 905.456 kb on + strand at 905.456 kb on + strand at 905.456 kb on + strand at 905.457 kb on - strand at 905.457 kb on - strand at 906.338 kb on + strand, within H281DRAFT_00817 at 906.339 kb on - strand, within H281DRAFT_00817 at 906.344 kb on + strand, within H281DRAFT_00817 at 906.443 kb on + strand, within H281DRAFT_00817 at 906.513 kb on - strand, within H281DRAFT_00817 at 906.892 kb on - strand, within H281DRAFT_00817 at 907.185 kb on - strand, within H281DRAFT_00817 at 907.218 kb on + strand, within H281DRAFT_00817 at 907.218 kb on + strand, within H281DRAFT_00817 at 907.222 kb on + strand, within H281DRAFT_00817 at 907.223 kb on - strand, within H281DRAFT_00817 at 907.223 kb on - strand, within H281DRAFT_00817 at 907.350 kb on - strand, within H281DRAFT_00817 at 907.359 kb on - strand, within H281DRAFT_00817 at 907.404 kb on - strand, within H281DRAFT_00817 at 907.997 kb on + strand at 907.997 kb on + strand at 907.998 kb on - strand at 908.072 kb on - strand at 908.175 kb on + strand at 908.175 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 904,625 + +0.4 904,629 + +0.6 904,724 + -1.3 904,725 - +1.0 904,725 - +0.1 904,733 + -0.7 904,734 - -0.5 904,734 - -1.1 904,734 - +0.3 904,734 - -0.0 904,749 + +1.9 904,750 - +0.6 904,750 - -0.3 904,777 + -0.4 904,780 - -0.6 904,780 - -1.0 904,822 + H281DRAFT_00816 0.17 -1.2 904,822 + H281DRAFT_00816 0.17 -0.2 904,823 - H281DRAFT_00816 0.17 -0.6 904,833 + H281DRAFT_00816 0.19 -1.9 905,170 + H281DRAFT_00816 0.85 +0.8 905,171 - H281DRAFT_00816 0.86 +0.1 905,171 - H281DRAFT_00816 0.86 -0.4 905,456 + +0.5 905,456 + -1.2 905,456 + +0.2 905,456 + -1.2 905,457 - -0.3 905,457 - +0.7 906,338 + H281DRAFT_00817 0.37 +0.4 906,339 - H281DRAFT_00817 0.37 +0.3 906,344 + H281DRAFT_00817 0.37 +0.2 906,443 + H281DRAFT_00817 0.41 +2.0 906,513 - H281DRAFT_00817 0.44 +0.6 906,892 - H281DRAFT_00817 0.58 +0.5 907,185 - H281DRAFT_00817 0.70 -0.6 907,218 + H281DRAFT_00817 0.71 +0.8 907,218 + H281DRAFT_00817 0.71 +1.4 907,222 + H281DRAFT_00817 0.71 +1.0 907,223 - H281DRAFT_00817 0.71 +0.5 907,223 - H281DRAFT_00817 0.71 +0.7 907,350 - H281DRAFT_00817 0.76 +0.9 907,359 - H281DRAFT_00817 0.76 -0.8 907,404 - H281DRAFT_00817 0.78 +1.3 907,997 + -0.8 907,997 + -0.1 907,998 - -0.8 908,072 - -0.3 908,175 + -0.2 908,175 + +1.6
Or see this region's nucleotide sequence