Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00764 and H281DRAFT_00765 are separated by 102 nucleotides H281DRAFT_00765 and H281DRAFT_00766 are separated by 372 nucleotides H281DRAFT_00766 and H281DRAFT_00767 are separated by 89 nucleotides H281DRAFT_00767 and H281DRAFT_00768 are separated by 91 nucleotides H281DRAFT_00768 and H281DRAFT_00769 are separated by 262 nucleotides
H281DRAFT_00764: H281DRAFT_00764 - hypothetical protein, at 854,491 to 854,733
_00764
H281DRAFT_00765: H281DRAFT_00765 - hypothetical protein, at 854,836 to 855,102
_00765
H281DRAFT_00766: H281DRAFT_00766 - tRNA-U16,U17-dihydrouridine synthase, at 855,475 to 856,428
_00766
H281DRAFT_00767: H281DRAFT_00767 - tRNA_His_GTG, at 856,518 to 856,593
_00767
H281DRAFT_00768: H281DRAFT_00768 - hypothetical protein, at 856,685 to 857,026
_00768
H281DRAFT_00769: H281DRAFT_00769 - hypothetical protein, at 857,289 to 857,501
_00769
Position (kb) 855
856
857 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 854.533 kb on + strand, within H281DRAFT_00764 at 854.542 kb on + strand, within H281DRAFT_00764 at 854.543 kb on - strand, within H281DRAFT_00764 at 854.837 kb on + strand at 854.838 kb on - strand at 854.919 kb on - strand, within H281DRAFT_00765 at 854.919 kb on - strand, within H281DRAFT_00765 at 855.008 kb on + strand, within H281DRAFT_00765 at 855.008 kb on + strand, within H281DRAFT_00765 at 855.009 kb on - strand, within H281DRAFT_00765 at 855.009 kb on - strand, within H281DRAFT_00765 at 855.009 kb on - strand, within H281DRAFT_00765 at 855.009 kb on - strand, within H281DRAFT_00765 at 855.027 kb on - strand, within H281DRAFT_00765 at 855.107 kb on + strand at 855.108 kb on - strand at 855.108 kb on - strand at 855.108 kb on - strand at 855.184 kb on + strand at 855.185 kb on - strand at 855.185 kb on - strand at 855.196 kb on - strand at 855.439 kb on - strand at 855.441 kb on + strand at 855.441 kb on + strand at 855.542 kb on - strand at 855.694 kb on + strand, within H281DRAFT_00766 at 855.927 kb on + strand, within H281DRAFT_00766 at 855.928 kb on - strand, within H281DRAFT_00766 at 856.001 kb on - strand, within H281DRAFT_00766 at 856.007 kb on - strand, within H281DRAFT_00766 at 856.012 kb on + strand, within H281DRAFT_00766 at 856.133 kb on - strand, within H281DRAFT_00766 at 856.144 kb on + strand, within H281DRAFT_00766 at 856.144 kb on + strand, within H281DRAFT_00766 at 856.145 kb on - strand, within H281DRAFT_00766 at 856.145 kb on - strand, within H281DRAFT_00766 at 856.145 kb on - strand, within H281DRAFT_00766 at 856.145 kb on - strand, within H281DRAFT_00766 at 856.147 kb on - strand, within H281DRAFT_00766 at 856.175 kb on - strand, within H281DRAFT_00766 at 856.175 kb on - strand, within H281DRAFT_00766 at 856.261 kb on + strand, within H281DRAFT_00766 at 856.261 kb on + strand, within H281DRAFT_00766 at 856.261 kb on + strand, within H281DRAFT_00766 at 856.261 kb on + strand, within H281DRAFT_00766 at 856.298 kb on - strand, within H281DRAFT_00766 at 856.375 kb on - strand at 856.375 kb on - strand at 856.375 kb on - strand at 856.375 kb on - strand at 856.608 kb on + strand at 856.831 kb on - strand, within H281DRAFT_00768 at 856.833 kb on + strand, within H281DRAFT_00768 at 856.833 kb on + strand, within H281DRAFT_00768 at 856.890 kb on + strand, within H281DRAFT_00768 at 856.914 kb on + strand, within H281DRAFT_00768 at 856.926 kb on + strand, within H281DRAFT_00768 at 856.926 kb on + strand, within H281DRAFT_00768 at 856.927 kb on - strand, within H281DRAFT_00768 at 856.927 kb on - strand, within H281DRAFT_00768 at 856.944 kb on + strand, within H281DRAFT_00768 at 856.945 kb on - strand, within H281DRAFT_00768 at 856.945 kb on - strand, within H281DRAFT_00768 at 857.047 kb on + strand at 857.047 kb on + strand at 857.048 kb on - strand at 857.048 kb on - strand at 857.048 kb on - strand at 857.048 kb on - strand at 857.048 kb on - strand at 857.102 kb on + strand at 857.151 kb on + strand at 857.201 kb on + strand at 857.201 kb on + strand at 857.202 kb on - strand at 857.222 kb on + strand at 857.257 kb on + strand at 857.258 kb on - strand at 857.258 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 854,533 + H281DRAFT_00764 0.17 -0.1 854,542 + H281DRAFT_00764 0.21 -2.0 854,543 - H281DRAFT_00764 0.21 -2.7 854,837 + -0.8 854,838 - +0.0 854,919 - H281DRAFT_00765 0.31 +0.1 854,919 - H281DRAFT_00765 0.31 -1.0 855,008 + H281DRAFT_00765 0.64 +0.8 855,008 + H281DRAFT_00765 0.64 +0.3 855,009 - H281DRAFT_00765 0.65 -0.5 855,009 - H281DRAFT_00765 0.65 +0.5 855,009 - H281DRAFT_00765 0.65 +0.4 855,009 - H281DRAFT_00765 0.65 -0.7 855,027 - H281DRAFT_00765 0.72 +0.1 855,107 + -0.5 855,108 - +0.2 855,108 - -0.3 855,108 - +0.2 855,184 + -0.9 855,185 - +0.3 855,185 - -0.9 855,196 - -0.2 855,439 - -0.9 855,441 + +0.5 855,441 + -0.0 855,542 - -2.2 855,694 + H281DRAFT_00766 0.23 -0.9 855,927 + H281DRAFT_00766 0.47 -0.7 855,928 - H281DRAFT_00766 0.47 +0.8 856,001 - H281DRAFT_00766 0.55 +1.7 856,007 - H281DRAFT_00766 0.56 +1.2 856,012 + H281DRAFT_00766 0.56 +0.6 856,133 - H281DRAFT_00766 0.69 -0.8 856,144 + H281DRAFT_00766 0.70 -2.4 856,144 + H281DRAFT_00766 0.70 +0.6 856,145 - H281DRAFT_00766 0.70 +1.3 856,145 - H281DRAFT_00766 0.70 -0.7 856,145 - H281DRAFT_00766 0.70 +0.8 856,145 - H281DRAFT_00766 0.70 -1.1 856,147 - H281DRAFT_00766 0.70 -0.2 856,175 - H281DRAFT_00766 0.73 -0.3 856,175 - H281DRAFT_00766 0.73 -0.3 856,261 + H281DRAFT_00766 0.82 -0.3 856,261 + H281DRAFT_00766 0.82 -0.4 856,261 + H281DRAFT_00766 0.82 -1.9 856,261 + H281DRAFT_00766 0.82 -0.5 856,298 - H281DRAFT_00766 0.86 -0.6 856,375 - -2.8 856,375 - -1.3 856,375 - +0.4 856,375 - -0.6 856,608 + +0.5 856,831 - H281DRAFT_00768 0.43 +0.1 856,833 + H281DRAFT_00768 0.43 +0.2 856,833 + H281DRAFT_00768 0.43 +0.3 856,890 + H281DRAFT_00768 0.60 +0.8 856,914 + H281DRAFT_00768 0.67 +0.4 856,926 + H281DRAFT_00768 0.70 +1.0 856,926 + H281DRAFT_00768 0.70 +0.3 856,927 - H281DRAFT_00768 0.71 +0.4 856,927 - H281DRAFT_00768 0.71 -0.7 856,944 + H281DRAFT_00768 0.76 +0.3 856,945 - H281DRAFT_00768 0.76 -1.0 856,945 - H281DRAFT_00768 0.76 +0.1 857,047 + +1.5 857,047 + -0.9 857,048 - +0.1 857,048 - -0.5 857,048 - +0.2 857,048 - -0.7 857,048 - -0.1 857,102 + +0.5 857,151 + +0.6 857,201 + +0.6 857,201 + -0.3 857,202 - +0.7 857,222 + +0.1 857,257 + -0.5 857,258 - -0.1 857,258 - -1.3
Or see this region's nucleotide sequence