Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00507 and H281DRAFT_00508 are separated by 17 nucleotides H281DRAFT_00508 and H281DRAFT_00509 are separated by 43 nucleotides H281DRAFT_00509 and H281DRAFT_00510 are separated by 124 nucleotides
H281DRAFT_00507: H281DRAFT_00507 - tryptophanyl-tRNA synthetase, at 567,807 to 569,009
_00507
H281DRAFT_00508: H281DRAFT_00508 - Methyltransferase domain-containing protein, at 569,027 to 569,623
_00508
H281DRAFT_00509: H281DRAFT_00509 - dihydrodipicolinate synthase, at 569,667 to 570,593
_00509
H281DRAFT_00510: H281DRAFT_00510 - Beta-barrel assembly machine subunit BamC, at 570,718 to 571,857
_00510
Position (kb) 569
570
571 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 569.002 kb on - strand at 569.002 kb on - strand at 569.314 kb on + strand, within H281DRAFT_00508 at 569.318 kb on + strand, within H281DRAFT_00508 at 569.318 kb on + strand, within H281DRAFT_00508 at 569.318 kb on + strand, within H281DRAFT_00508 at 569.319 kb on - strand, within H281DRAFT_00508 at 569.319 kb on - strand, within H281DRAFT_00508 at 569.381 kb on + strand, within H281DRAFT_00508 at 569.425 kb on + strand, within H281DRAFT_00508 at 569.426 kb on - strand, within H281DRAFT_00508 at 569.607 kb on - strand at 571.010 kb on - strand, within H281DRAFT_00510 at 571.482 kb on + strand, within H281DRAFT_00510 at 571.515 kb on + strand, within H281DRAFT_00510 at 571.515 kb on + strand, within H281DRAFT_00510
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 569,002 - -3.3 569,002 - -0.7 569,314 + H281DRAFT_00508 0.48 -0.6 569,318 + H281DRAFT_00508 0.49 +0.9 569,318 + H281DRAFT_00508 0.49 +0.1 569,318 + H281DRAFT_00508 0.49 +0.5 569,319 - H281DRAFT_00508 0.49 +0.9 569,319 - H281DRAFT_00508 0.49 +0.9 569,381 + H281DRAFT_00508 0.59 +1.0 569,425 + H281DRAFT_00508 0.67 +0.1 569,426 - H281DRAFT_00508 0.67 +0.7 569,607 - +0.3 571,010 - H281DRAFT_00510 0.26 -1.7 571,482 + H281DRAFT_00510 0.67 -2.6 571,515 + H281DRAFT_00510 0.70 -0.3 571,515 + H281DRAFT_00510 0.70 -0.7
Or see this region's nucleotide sequence