Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00315 and H281DRAFT_00316 are separated by 170 nucleotides H281DRAFT_00316 and H281DRAFT_00317 overlap by 1 nucleotides
H281DRAFT_00315: H281DRAFT_00315 - Glycosyltransferase involved in cell wall bisynthesis, at 342,706 to 343,785
_00315
H281DRAFT_00316: H281DRAFT_00316 - ADP-heptose:LPS heptosyltransferase, at 343,956 to 345,275
_00316
H281DRAFT_00317: H281DRAFT_00317 - O-antigen ligase, at 345,275 to 346,630
_00317
Position (kb) 343
344
345
346 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 343.159 kb on + strand, within H281DRAFT_00315 at 343.160 kb on - strand, within H281DRAFT_00315 at 343.160 kb on - strand, within H281DRAFT_00315 at 343.354 kb on - strand, within H281DRAFT_00315 at 343.354 kb on - strand, within H281DRAFT_00315 at 343.430 kb on - strand, within H281DRAFT_00315 at 343.474 kb on - strand, within H281DRAFT_00315 at 343.784 kb on - strand at 343.784 kb on - strand at 343.940 kb on - strand at 344.057 kb on + strand at 344.108 kb on - strand, within H281DRAFT_00316 at 344.147 kb on + strand, within H281DRAFT_00316 at 344.148 kb on - strand, within H281DRAFT_00316 at 344.368 kb on + strand, within H281DRAFT_00316 at 344.368 kb on + strand, within H281DRAFT_00316 at 344.369 kb on - strand, within H281DRAFT_00316 at 344.371 kb on - strand, within H281DRAFT_00316 at 344.458 kb on - strand, within H281DRAFT_00316 at 344.493 kb on - strand, within H281DRAFT_00316 at 344.589 kb on + strand, within H281DRAFT_00316 at 344.590 kb on - strand, within H281DRAFT_00316 at 344.635 kb on + strand, within H281DRAFT_00316 at 344.635 kb on + strand at 344.636 kb on - strand, within H281DRAFT_00316 at 344.727 kb on + strand, within H281DRAFT_00316 at 344.727 kb on + strand, within H281DRAFT_00316 at 344.727 kb on + strand at 344.727 kb on + strand, within H281DRAFT_00316 at 344.728 kb on - strand, within H281DRAFT_00316 at 344.821 kb on - strand, within H281DRAFT_00316 at 345.008 kb on + strand, within H281DRAFT_00316 at 345.009 kb on - strand, within H281DRAFT_00316 at 345.009 kb on - strand, within H281DRAFT_00316 at 345.126 kb on - strand, within H281DRAFT_00316 at 345.142 kb on + strand, within H281DRAFT_00316 at 345.142 kb on + strand, within H281DRAFT_00316 at 345.143 kb on - strand, within H281DRAFT_00316 at 345.257 kb on + strand at 345.258 kb on - strand at 345.274 kb on - strand at 345.555 kb on - strand, within H281DRAFT_00317 at 345.722 kb on + strand, within H281DRAFT_00317 at 345.740 kb on - strand, within H281DRAFT_00317 at 345.907 kb on - strand, within H281DRAFT_00317 at 346.142 kb on + strand, within H281DRAFT_00317 at 346.142 kb on + strand, within H281DRAFT_00317 at 346.211 kb on + strand, within H281DRAFT_00317
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 343,159 + H281DRAFT_00315 0.42 +1.9 343,160 - H281DRAFT_00315 0.42 +0.9 343,160 - H281DRAFT_00315 0.42 +1.1 343,354 - H281DRAFT_00315 0.60 +1.2 343,354 - H281DRAFT_00315 0.60 +1.5 343,430 - H281DRAFT_00315 0.67 +1.8 343,474 - H281DRAFT_00315 0.71 +1.2 343,784 - +0.1 343,784 - +0.8 343,940 - -1.8 344,057 + -0.4 344,108 - H281DRAFT_00316 0.12 -0.3 344,147 + H281DRAFT_00316 0.14 -0.1 344,148 - H281DRAFT_00316 0.15 -0.4 344,368 + H281DRAFT_00316 0.31 +2.0 344,368 + H281DRAFT_00316 0.31 +2.3 344,369 - H281DRAFT_00316 0.31 +1.7 344,371 - H281DRAFT_00316 0.31 +0.9 344,458 - H281DRAFT_00316 0.38 +1.4 344,493 - H281DRAFT_00316 0.41 -0.5 344,589 + H281DRAFT_00316 0.48 +3.3 344,590 - H281DRAFT_00316 0.48 +2.3 344,635 + H281DRAFT_00316 0.51 +2.5 344,635 + +3.7 344,636 - H281DRAFT_00316 0.52 +2.8 344,727 + H281DRAFT_00316 0.58 +2.0 344,727 + H281DRAFT_00316 0.58 +0.5 344,727 + +2.7 344,727 + H281DRAFT_00316 0.58 +1.8 344,728 - H281DRAFT_00316 0.58 +2.2 344,821 - H281DRAFT_00316 0.66 +3.9 345,008 + H281DRAFT_00316 0.80 +2.0 345,009 - H281DRAFT_00316 0.80 +3.7 345,009 - H281DRAFT_00316 0.80 +3.4 345,126 - H281DRAFT_00316 0.89 +1.3 345,142 + H281DRAFT_00316 0.90 +2.5 345,142 + H281DRAFT_00316 0.90 +1.6 345,143 - H281DRAFT_00316 0.90 +2.2 345,257 + -1.4 345,258 - -1.1 345,274 - +0.4 345,555 - H281DRAFT_00317 0.21 +4.0 345,722 + H281DRAFT_00317 0.33 +5.9 345,740 - H281DRAFT_00317 0.34 +4.5 345,907 - H281DRAFT_00317 0.47 +3.7 346,142 + H281DRAFT_00317 0.64 +2.8 346,142 + H281DRAFT_00317 0.64 +3.5 346,211 + H281DRAFT_00317 0.69 +3.4
Or see this region's nucleotide sequence