Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00033 and H281DRAFT_00034 are separated by 676 nucleotides H281DRAFT_00034 and H281DRAFT_00035 are separated by 547 nucleotides
H281DRAFT_00033: H281DRAFT_00033 - IS66 C-terminal element, at 35,026 to 35,640
_00033
H281DRAFT_00034: H281DRAFT_00034 - hypothetical protein, at 36,317 to 36,478
_00034
H281DRAFT_00035: H281DRAFT_00035 - Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family, at 37,026 to 37,439
_00035
Position (kb) 36
37 Strain fitness (log2 ratio) -1
0
1
2 at 35.388 kb on - strand, within H281DRAFT_00033 at 35.545 kb on + strand, within H281DRAFT_00033 at 35.818 kb on + strand at 35.818 kb on + strand at 35.932 kb on - strand at 36.028 kb on + strand at 36.148 kb on + strand at 36.151 kb on - strand at 36.259 kb on - strand at 36.292 kb on - strand at 36.333 kb on - strand at 36.340 kb on + strand, within H281DRAFT_00034 at 36.478 kb on + strand at 36.479 kb on - strand at 36.572 kb on + strand at 36.580 kb on + strand at 36.643 kb on + strand at 36.771 kb on + strand at 36.771 kb on + strand at 36.772 kb on - strand at 36.838 kb on - strand at 36.901 kb on - strand at 36.901 kb on - strand at 36.904 kb on + strand at 36.904 kb on + strand at 36.905 kb on - strand at 36.905 kb on - strand at 36.972 kb on + strand at 36.972 kb on + strand at 36.973 kb on - strand at 37.027 kb on + strand at 37.028 kb on - strand at 37.069 kb on + strand, within H281DRAFT_00035 at 37.069 kb on + strand, within H281DRAFT_00035 at 37.069 kb on + strand, within H281DRAFT_00035 at 37.070 kb on - strand, within H281DRAFT_00035 at 37.070 kb on - strand, within H281DRAFT_00035 at 37.070 kb on - strand, within H281DRAFT_00035 at 37.070 kb on - strand, within H281DRAFT_00035 at 37.093 kb on - strand, within H281DRAFT_00035 at 37.111 kb on + strand, within H281DRAFT_00035 at 37.244 kb on + strand, within H281DRAFT_00035 at 37.267 kb on + strand, within H281DRAFT_00035 at 37.268 kb on - strand, within H281DRAFT_00035
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 35,388 - H281DRAFT_00033 0.59 +0.9 35,545 + H281DRAFT_00033 0.84 +0.5 35,818 + -0.3 35,818 + -0.6 35,932 - +0.7 36,028 + +0.1 36,148 + +0.6 36,151 - +1.1 36,259 - -0.6 36,292 - +2.5 36,333 - +2.7 36,340 + H281DRAFT_00034 0.14 -0.1 36,478 + +0.0 36,479 - -0.4 36,572 + -0.5 36,580 + +0.7 36,643 + +0.2 36,771 + -0.5 36,771 + +1.2 36,772 - -1.2 36,838 - +1.0 36,901 - +0.7 36,901 - -0.7 36,904 + +0.5 36,904 + -0.1 36,905 - +0.7 36,905 - +0.8 36,972 + +0.6 36,972 + +0.5 36,973 - +1.2 37,027 + -0.2 37,028 - -0.1 37,069 + H281DRAFT_00035 0.10 +0.3 37,069 + H281DRAFT_00035 0.10 +0.1 37,069 + H281DRAFT_00035 0.10 +0.4 37,070 - H281DRAFT_00035 0.11 +1.2 37,070 - H281DRAFT_00035 0.11 -0.4 37,070 - H281DRAFT_00035 0.11 +0.2 37,070 - H281DRAFT_00035 0.11 +0.5 37,093 - H281DRAFT_00035 0.16 +0.4 37,111 + H281DRAFT_00035 0.21 +0.1 37,244 + H281DRAFT_00035 0.53 -0.2 37,267 + H281DRAFT_00035 0.58 -0.1 37,268 - H281DRAFT_00035 0.58 +2.0
Or see this region's nucleotide sequence