Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_73 and Ac3H11_74 are separated by 27 nucleotides Ac3H11_74 and Ac3H11_75 are separated by 111 nucleotides Ac3H11_75 and Ac3H11_76 are separated by 8 nucleotides Ac3H11_76 and Ac3H11_77 are separated by 24 nucleotides Ac3H11_77 and Ac3H11_78 overlap by 4 nucleotides
Ac3H11_73 - Phenylalanyl-tRNA synthetase beta subunit, at 77,407 to 77,874
_73
Ac3H11_74 - hypothetical protein, at 77,902 to 78,144
_74
Ac3H11_75 - Outer membrane lipoprotein blc precursor, at 78,256 to 78,822
_75
Ac3H11_76 - COG3380: Amine oxidase, flavin-containing, at 78,831 to 79,835
_76
Ac3H11_77 - Gsl3115 protein, at 79,860 to 80,036
_77
Ac3H11_78 - Cryptochrome, at 80,033 to 81,349
_78
Position (kb) 78
79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 77.866 kb on + strand at 77.866 kb on + strand at 77.867 kb on - strand at 77.867 kb on - strand at 77.872 kb on + strand at 77.873 kb on - strand at 78.012 kb on - strand, within Ac3H11_74 at 78.012 kb on - strand, within Ac3H11_74 at 78.123 kb on - strand at 78.155 kb on + strand at 78.255 kb on + strand at 78.466 kb on + strand, within Ac3H11_75 at 78.467 kb on - strand, within Ac3H11_75 at 78.467 kb on - strand, within Ac3H11_75 at 78.613 kb on + strand, within Ac3H11_75 at 78.622 kb on + strand, within Ac3H11_75 at 78.649 kb on + strand, within Ac3H11_75 at 78.685 kb on + strand, within Ac3H11_75 at 78.685 kb on + strand, within Ac3H11_75 at 78.685 kb on + strand, within Ac3H11_75 at 78.686 kb on - strand, within Ac3H11_75 at 78.686 kb on - strand, within Ac3H11_75 at 78.830 kb on + strand at 78.830 kb on + strand at 78.830 kb on + strand at 78.831 kb on - strand at 78.831 kb on - strand at 79.014 kb on + strand, within Ac3H11_76 at 79.684 kb on - strand, within Ac3H11_76 at 79.833 kb on + strand at 79.834 kb on - strand at 79.860 kb on - strand at 79.959 kb on + strand, within Ac3H11_77 at 79.960 kb on - strand, within Ac3H11_77 at 80.358 kb on - strand, within Ac3H11_78 at 80.408 kb on - strand, within Ac3H11_78 at 80.726 kb on + strand, within Ac3H11_78 at 80.727 kb on - strand, within Ac3H11_78 at 80.754 kb on - strand, within Ac3H11_78 at 80.789 kb on + strand, within Ac3H11_78 at 80.790 kb on - strand, within Ac3H11_78
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 77,866 + -0.6 77,866 + +2.1 77,867 - -1.4 77,867 - +0.9 77,872 + +3.0 77,873 - -3.6 78,012 - Ac3H11_74 0.45 +0.2 78,012 - Ac3H11_74 0.45 -0.0 78,123 - -0.4 78,155 + -0.0 78,255 + -0.0 78,466 + Ac3H11_75 0.37 +0.7 78,467 - Ac3H11_75 0.37 -2.2 78,467 - Ac3H11_75 0.37 +0.7 78,613 + Ac3H11_75 0.63 -0.2 78,622 + Ac3H11_75 0.65 -1.4 78,649 + Ac3H11_75 0.69 -0.6 78,685 + Ac3H11_75 0.76 +0.1 78,685 + Ac3H11_75 0.76 +0.2 78,685 + Ac3H11_75 0.76 -0.4 78,686 - Ac3H11_75 0.76 +2.0 78,686 - Ac3H11_75 0.76 -2.2 78,830 + -1.4 78,830 + +1.0 78,830 + -3.0 78,831 - +0.7 78,831 - +1.2 79,014 + Ac3H11_76 0.18 +2.0 79,684 - Ac3H11_76 0.85 +0.4 79,833 + -1.5 79,834 - +0.2 79,860 - -2.6 79,959 + Ac3H11_77 0.56 -0.2 79,960 - Ac3H11_77 0.56 -1.0 80,358 - Ac3H11_78 0.25 +1.5 80,408 - Ac3H11_78 0.28 -0.1 80,726 + Ac3H11_78 0.53 +1.3 80,727 - Ac3H11_78 0.53 -3.4 80,754 - Ac3H11_78 0.55 -1.1 80,789 + Ac3H11_78 0.57 -0.9 80,790 - Ac3H11_78 0.57 -0.3
Or see this region's nucleotide sequence