Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3727 and Ac3H11_3728 are separated by 29 nucleotides Ac3H11_3728 and Ac3H11_3729 overlap by 4 nucleotides Ac3H11_3729 and Ac3H11_3730 are separated by 23 nucleotides Ac3H11_3730 and Ac3H11_3731 are separated by 25 nucleotides
Ac3H11_3727 - Creatinine amidohydrolase (EC 3.5.2.10), at 87,448 to 88,311
_3727
Ac3H11_3728 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 88,341 to 88,805
_3728
Ac3H11_3729 - Cytosine deaminase (EC 3.5.4.1), at 88,802 to 90,130
_3729
Ac3H11_3730 - Glutathione-regulated potassium-efflux system ancillary protein KefG, at 90,154 to 90,741
_3730
Ac3H11_3731 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 90,767 to 91,069
_3731
Position (kb) 88
89
90
91 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 87.981 kb on - strand, within Ac3H11_3727 at 88.076 kb on + strand, within Ac3H11_3727 at 88.076 kb on + strand, within Ac3H11_3727 at 88.077 kb on - strand, within Ac3H11_3727 at 88.077 kb on - strand, within Ac3H11_3727 at 88.298 kb on + strand at 88.298 kb on + strand at 88.298 kb on + strand at 88.299 kb on - strand at 88.299 kb on - strand at 88.357 kb on + strand at 88.357 kb on + strand at 88.357 kb on + strand at 88.501 kb on + strand, within Ac3H11_3728 at 88.501 kb on + strand, within Ac3H11_3728 at 88.502 kb on - strand, within Ac3H11_3728 at 88.502 kb on - strand, within Ac3H11_3728 at 88.576 kb on + strand, within Ac3H11_3728 at 88.576 kb on + strand, within Ac3H11_3728 at 88.576 kb on + strand, within Ac3H11_3728 at 88.577 kb on - strand, within Ac3H11_3728 at 88.577 kb on - strand, within Ac3H11_3728 at 88.577 kb on - strand, within Ac3H11_3728 at 88.577 kb on - strand, within Ac3H11_3728 at 88.727 kb on - strand, within Ac3H11_3728 at 89.169 kb on - strand, within Ac3H11_3729 at 89.169 kb on - strand, within Ac3H11_3729 at 90.155 kb on + strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.233 kb on + strand, within Ac3H11_3730 at 90.233 kb on + strand, within Ac3H11_3730 at 90.234 kb on - strand, within Ac3H11_3730 at 90.237 kb on - strand, within Ac3H11_3730 at 90.237 kb on - strand, within Ac3H11_3730 at 90.245 kb on + strand, within Ac3H11_3730 at 90.285 kb on - strand, within Ac3H11_3730 at 90.285 kb on - strand, within Ac3H11_3730 at 90.285 kb on - strand, within Ac3H11_3730 at 90.348 kb on - strand, within Ac3H11_3730 at 90.348 kb on - strand, within Ac3H11_3730 at 90.348 kb on - strand, within Ac3H11_3730 at 90.536 kb on + strand, within Ac3H11_3730 at 90.537 kb on - strand, within Ac3H11_3730 at 90.623 kb on + strand, within Ac3H11_3730 at 90.699 kb on - strand at 90.710 kb on - strand at 90.722 kb on + strand at 90.722 kb on + strand at 90.722 kb on + strand at 90.723 kb on - strand at 90.723 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 87,981 - Ac3H11_3727 0.62 +1.2 88,076 + Ac3H11_3727 0.73 -0.8 88,076 + Ac3H11_3727 0.73 +0.1 88,077 - Ac3H11_3727 0.73 -0.3 88,077 - Ac3H11_3727 0.73 +1.8 88,298 + +1.5 88,298 + +1.2 88,298 + +1.0 88,299 - -1.6 88,299 - +1.2 88,357 + +1.6 88,357 + -1.9 88,357 + -0.5 88,501 + Ac3H11_3728 0.34 -0.8 88,501 + Ac3H11_3728 0.34 -1.8 88,502 - Ac3H11_3728 0.35 -1.5 88,502 - Ac3H11_3728 0.35 -3.4 88,576 + Ac3H11_3728 0.51 +0.2 88,576 + Ac3H11_3728 0.51 -0.5 88,576 + Ac3H11_3728 0.51 -0.8 88,577 - Ac3H11_3728 0.51 +0.1 88,577 - Ac3H11_3728 0.51 -0.5 88,577 - Ac3H11_3728 0.51 +2.4 88,577 - Ac3H11_3728 0.51 -1.2 88,727 - Ac3H11_3728 0.83 -0.7 89,169 - Ac3H11_3729 0.28 +1.1 89,169 - Ac3H11_3729 0.28 +0.5 90,155 + -0.8 90,156 - -1.6 90,156 - +1.5 90,156 - +0.1 90,156 - -2.2 90,156 - -1.6 90,233 + Ac3H11_3730 0.13 +1.4 90,233 + Ac3H11_3730 0.13 -2.8 90,234 - Ac3H11_3730 0.14 -1.8 90,237 - Ac3H11_3730 0.14 +0.2 90,237 - Ac3H11_3730 0.14 -1.8 90,245 + Ac3H11_3730 0.15 +1.0 90,285 - Ac3H11_3730 0.22 -2.2 90,285 - Ac3H11_3730 0.22 +2.1 90,285 - Ac3H11_3730 0.22 +2.2 90,348 - Ac3H11_3730 0.33 -0.1 90,348 - Ac3H11_3730 0.33 -0.5 90,348 - Ac3H11_3730 0.33 +1.0 90,536 + Ac3H11_3730 0.65 -2.2 90,537 - Ac3H11_3730 0.65 +0.4 90,623 + Ac3H11_3730 0.80 +0.1 90,699 - -0.8 90,710 - -0.4 90,722 + +3.2 90,722 + -1.0 90,722 + +4.1 90,723 - +1.6 90,723 - +1.7
Or see this region's nucleotide sequence