Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_916 and Pf1N1B4_917 are separated by 119 nucleotides Pf1N1B4_917 and Pf1N1B4_918 are separated by 469 nucleotides
Pf1N1B4_916 - Glutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4), at 1,041,102 to 1,042,229
_916
Pf1N1B4_917 - Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4), at 1,042,349 to 1,043,380
_917
Pf1N1B4_918 - phospholipase/carboxylesterase family protein, at 1,043,850 to 1,044,506
_918
Position (kb)
1042
1043
1044 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1041.472 kb on + strand, within Pf1N1B4_916 at 1041.472 kb on + strand, within Pf1N1B4_916 at 1041.472 kb on + strand, within Pf1N1B4_916 at 1041.472 kb on + strand, within Pf1N1B4_916 at 1041.473 kb on - strand, within Pf1N1B4_916 at 1041.809 kb on + strand, within Pf1N1B4_916 at 1041.900 kb on + strand, within Pf1N1B4_916 at 1041.900 kb on + strand, within Pf1N1B4_916 at 1041.901 kb on - strand, within Pf1N1B4_916 at 1041.901 kb on - strand, within Pf1N1B4_916 at 1041.901 kb on - strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.904 kb on + strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1041.905 kb on - strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.045 kb on + strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.046 kb on - strand, within Pf1N1B4_916 at 1042.063 kb on + strand, within Pf1N1B4_916 at 1042.064 kb on - strand, within Pf1N1B4_916 at 1042.064 kb on - strand, within Pf1N1B4_916 at 1042.127 kb on + strand at 1042.127 kb on + strand at 1042.128 kb on - strand at 1042.171 kb on + strand at 1042.259 kb on + strand at 1042.259 kb on + strand at 1042.259 kb on + strand at 1042.259 kb on + strand at 1042.260 kb on - strand at 1042.260 kb on - strand at 1042.299 kb on + strand at 1042.299 kb on + strand at 1042.300 kb on - strand at 1042.341 kb on + strand at 1042.341 kb on + strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.342 kb on - strand at 1042.369 kb on - strand at 1042.426 kb on + strand at 1042.427 kb on - strand at 1042.427 kb on - strand at 1042.461 kb on + strand, within Pf1N1B4_917 at 1042.462 kb on - strand, within Pf1N1B4_917 at 1042.462 kb on - strand, within Pf1N1B4_917 at 1042.462 kb on - strand, within Pf1N1B4_917 at 1042.462 kb on - strand, within Pf1N1B4_917 at 1042.462 kb on - strand, within Pf1N1B4_917 at 1042.547 kb on - strand, within Pf1N1B4_917 at 1042.547 kb on - strand, within Pf1N1B4_917 at 1042.547 kb on - strand, within Pf1N1B4_917 at 1042.547 kb on - strand, within Pf1N1B4_917 at 1042.547 kb on - strand, within Pf1N1B4_917 at 1042.547 kb on - strand, within Pf1N1B4_917 at 1042.547 kb on - strand, within Pf1N1B4_917 at 1042.547 kb on - strand, within Pf1N1B4_917 at 1042.679 kb on - strand, within Pf1N1B4_917 at 1042.731 kb on + strand at 1042.732 kb on - strand at 1042.732 kb on - strand, within Pf1N1B4_917 at 1042.732 kb on - strand, within Pf1N1B4_917 at 1042.732 kb on - strand, within Pf1N1B4_917 at 1042.732 kb on - strand, within Pf1N1B4_917 at 1042.860 kb on + strand, within Pf1N1B4_917 at 1042.860 kb on + strand, within Pf1N1B4_917 at 1042.861 kb on - strand, within Pf1N1B4_917 at 1042.861 kb on - strand, within Pf1N1B4_917 at 1042.885 kb on - strand, within Pf1N1B4_917 at 1043.001 kb on + strand, within Pf1N1B4_917 at 1043.001 kb on + strand, within Pf1N1B4_917 at 1043.004 kb on + strand, within Pf1N1B4_917 at 1043.004 kb on + strand, within Pf1N1B4_917 at 1043.004 kb on + strand, within Pf1N1B4_917 at 1043.005 kb on - strand, within Pf1N1B4_917 at 1043.005 kb on - strand at 1043.005 kb on - strand, within Pf1N1B4_917 at 1043.005 kb on - strand, within Pf1N1B4_917 at 1043.255 kb on + strand, within Pf1N1B4_917 at 1043.255 kb on + strand, within Pf1N1B4_917 at 1043.256 kb on - strand, within Pf1N1B4_917 at 1043.256 kb on - strand, within Pf1N1B4_917 at 1043.469 kb on - strand at 1043.483 kb on + strand at 1043.483 kb on + strand at 1043.484 kb on - strand at 1043.484 kb on - strand at 1043.504 kb on + strand at 1043.505 kb on - strand at 1043.505 kb on - strand at 1043.505 kb on - strand at 1043.505 kb on - strand at 1043.508 kb on - strand at 1043.508 kb on - strand at 1043.530 kb on + strand at 1043.530 kb on + strand at 1043.531 kb on - strand at 1043.531 kb on - strand at 1043.565 kb on - strand at 1043.565 kb on - strand at 1043.646 kb on + strand at 1043.646 kb on + strand at 1043.647 kb on - strand at 1043.647 kb on - strand at 1043.647 kb on - strand at 1043.647 kb on - strand at 1043.647 kb on - strand at 1043.647 kb on - strand at 1043.647 kb on - strand at 1043.647 kb on - strand at 1043.647 kb on - strand at 1043.647 kb on - strand at 1043.654 kb on + strand at 1043.654 kb on + strand at 1043.655 kb on - strand at 1043.655 kb on - strand at 1043.655 kb on - strand at 1043.655 kb on - strand at 1043.655 kb on - strand at 1043.655 kb on - strand at 1043.655 kb on - strand at 1043.670 kb on + strand at 1043.671 kb on - strand at 1043.671 kb on - strand at 1043.671 kb on - strand at 1043.671 kb on - strand at 1043.671 kb on - strand at 1043.780 kb on + strand at 1043.781 kb on - strand at 1043.794 kb on - strand at 1043.794 kb on - strand at 1043.794 kb on - strand at 1043.817 kb on + strand at 1043.818 kb on - strand at 1043.818 kb on - strand at 1043.818 kb on - strand at 1043.818 kb on - strand at 1043.993 kb on - strand, within Pf1N1B4_918 at 1044.051 kb on + strand, within Pf1N1B4_918 at 1044.058 kb on + strand, within Pf1N1B4_918 at 1044.059 kb on - strand, within Pf1N1B4_918 at 1044.059 kb on - strand, within Pf1N1B4_918 at 1044.059 kb on - strand, within Pf1N1B4_918 at 1044.059 kb on - strand, within Pf1N1B4_918 at 1044.158 kb on - strand, within Pf1N1B4_918 at 1044.158 kb on - strand, within Pf1N1B4_918 at 1044.208 kb on + strand, within Pf1N1B4_918 at 1044.209 kb on - strand, within Pf1N1B4_918 at 1044.209 kb on - strand, within Pf1N1B4_918 at 1044.253 kb on + strand, within Pf1N1B4_918 at 1044.253 kb on + strand, within Pf1N1B4_918 at 1044.254 kb on - strand, within Pf1N1B4_918 at 1044.254 kb on - strand, within Pf1N1B4_918 at 1044.254 kb on - strand, within Pf1N1B4_918 at 1044.254 kb on - strand, within Pf1N1B4_918 at 1044.270 kb on + strand, within Pf1N1B4_918 at 1044.270 kb on + strand, within Pf1N1B4_918 at 1044.271 kb on - strand, within Pf1N1B4_918 at 1044.271 kb on - strand, within Pf1N1B4_918 at 1044.348 kb on + strand at 1044.348 kb on + strand, within Pf1N1B4_918 at 1044.349 kb on - strand, within Pf1N1B4_918
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 1,041,472 + Pf1N1B4_916 0.33 -0.9 1,041,472 + Pf1N1B4_916 0.33 +0.1 1,041,472 + Pf1N1B4_916 0.33 -0.3 1,041,472 + Pf1N1B4_916 0.33 +0.1 1,041,473 - Pf1N1B4_916 0.33 -1.3 1,041,809 + Pf1N1B4_916 0.63 -0.9 1,041,900 + Pf1N1B4_916 0.71 +0.1 1,041,900 + Pf1N1B4_916 0.71 -1.2 1,041,901 - Pf1N1B4_916 0.71 -1.7 1,041,901 - Pf1N1B4_916 0.71 -0.9 1,041,901 - Pf1N1B4_916 0.71 +0.1 1,041,904 + Pf1N1B4_916 0.71 +1.1 1,041,904 + +1.1 1,041,904 + Pf1N1B4_916 0.71 -1.7 1,041,904 + Pf1N1B4_916 0.71 +0.7 1,041,904 + Pf1N1B4_916 0.71 -0.3 1,041,904 + Pf1N1B4_916 0.71 -0.5 1,041,904 + Pf1N1B4_916 0.71 +0.7 1,041,904 + Pf1N1B4_916 0.71 -1.3 1,041,904 + Pf1N1B4_916 0.71 +0.1 1,041,904 + Pf1N1B4_916 0.71 +0.1 1,041,904 + Pf1N1B4_916 0.71 +0.1 1,041,904 + Pf1N1B4_916 0.71 +0.7 1,041,904 + Pf1N1B4_916 0.71 -0.9 1,041,904 + Pf1N1B4_916 0.71 -1.7 1,041,904 + +2.4 1,041,904 + Pf1N1B4_916 0.71 -0.9 1,041,904 + Pf1N1B4_916 0.71 +2.4 1,041,904 + Pf1N1B4_916 0.71 -1.9 1,041,904 + Pf1N1B4_916 0.71 +0.1 1,041,905 - Pf1N1B4_916 0.71 -1.5 1,041,905 - Pf1N1B4_916 0.71 -0.3 1,041,905 - Pf1N1B4_916 0.71 -0.3 1,041,905 - Pf1N1B4_916 0.71 -0.8 1,041,905 - Pf1N1B4_916 0.71 +1.4 1,041,905 - Pf1N1B4_916 0.71 -0.5 1,041,905 - Pf1N1B4_916 0.71 -1.3 1,041,905 - Pf1N1B4_916 0.71 +0.1 1,041,905 - Pf1N1B4_916 0.71 +0.1 1,041,905 - Pf1N1B4_916 0.71 -0.9 1,041,905 - Pf1N1B4_916 0.71 -2.5 1,041,905 - Pf1N1B4_916 0.71 +0.1 1,041,905 - Pf1N1B4_916 0.71 -0.9 1,041,905 - Pf1N1B4_916 0.71 -0.5 1,041,905 - Pf1N1B4_916 0.71 -0.7 1,041,905 - Pf1N1B4_916 0.71 -0.6 1,041,905 - Pf1N1B4_916 0.71 -1.5 1,041,905 - Pf1N1B4_916 0.71 -0.9 1,042,045 + Pf1N1B4_916 0.84 -1.0 1,042,045 + Pf1N1B4_916 0.84 -1.2 1,042,045 + +1.1 1,042,045 + Pf1N1B4_916 0.84 -0.3 1,042,045 + Pf1N1B4_916 0.84 -1.9 1,042,045 + Pf1N1B4_916 0.84 -1.5 1,042,045 + Pf1N1B4_916 0.84 +0.1 1,042,045 + Pf1N1B4_916 0.84 -0.7 1,042,045 + Pf1N1B4_916 0.84 -0.9 1,042,045 + Pf1N1B4_916 0.84 -0.3 1,042,045 + Pf1N1B4_916 0.84 -0.7 1,042,045 + Pf1N1B4_916 0.84 +0.1 1,042,045 + Pf1N1B4_916 0.84 +0.1 1,042,045 + Pf1N1B4_916 0.84 -1.5 1,042,046 - Pf1N1B4_916 0.84 -0.9 1,042,046 - Pf1N1B4_916 0.84 +1.1 1,042,046 - Pf1N1B4_916 0.84 -0.7 1,042,046 - Pf1N1B4_916 0.84 +1.7 1,042,046 - Pf1N1B4_916 0.84 +1.1 1,042,046 - Pf1N1B4_916 0.84 +0.1 1,042,046 - Pf1N1B4_916 0.84 -0.3 1,042,063 + Pf1N1B4_916 0.85 +0.1 1,042,064 - Pf1N1B4_916 0.85 -0.9 1,042,064 - Pf1N1B4_916 0.85 -0.2 1,042,127 + +0.9 1,042,127 + -1.4 1,042,128 - +0.1 1,042,171 + -0.3 1,042,259 + +0.4 1,042,259 + -1.3 1,042,259 + +0.1 1,042,259 + -0.9 1,042,260 - +0.1 1,042,260 - -0.9 1,042,299 + -0.3 1,042,299 + +0.3 1,042,300 - +1.9 1,042,341 + +0.1 1,042,341 + +0.1 1,042,342 - +0.7 1,042,342 - +0.7 1,042,342 - +0.3 1,042,342 - +0.4 1,042,342 - +0.5 1,042,342 - +0.8 1,042,342 - -1.3 1,042,342 - -0.6 1,042,342 - +1.0 1,042,342 - +0.4 1,042,342 - +0.1 1,042,342 - +1.5 1,042,342 - +0.7 1,042,369 - -0.5 1,042,426 + -1.9 1,042,427 - +0.1 1,042,427 - -0.9 1,042,461 + Pf1N1B4_917 0.11 -1.7 1,042,462 - Pf1N1B4_917 0.11 +0.1 1,042,462 - Pf1N1B4_917 0.11 +0.1 1,042,462 - Pf1N1B4_917 0.11 -1.5 1,042,462 - Pf1N1B4_917 0.11 -1.7 1,042,462 - Pf1N1B4_917 0.11 -0.9 1,042,547 - Pf1N1B4_917 0.19 -0.9 1,042,547 - Pf1N1B4_917 0.19 +0.1 1,042,547 - Pf1N1B4_917 0.19 -0.7 1,042,547 - Pf1N1B4_917 0.19 +1.4 1,042,547 - Pf1N1B4_917 0.19 -1.5 1,042,547 - Pf1N1B4_917 0.19 -1.5 1,042,547 - Pf1N1B4_917 0.19 -1.2 1,042,547 - Pf1N1B4_917 0.19 -0.6 1,042,679 - Pf1N1B4_917 0.32 +0.3 1,042,731 + -1.9 1,042,732 - +0.1 1,042,732 - Pf1N1B4_917 0.37 +1.2 1,042,732 - Pf1N1B4_917 0.37 +0.1 1,042,732 - Pf1N1B4_917 0.37 -0.3 1,042,732 - Pf1N1B4_917 0.37 +0.1 1,042,860 + Pf1N1B4_917 0.50 +0.1 1,042,860 + Pf1N1B4_917 0.50 +0.3 1,042,861 - Pf1N1B4_917 0.50 -0.3 1,042,861 - Pf1N1B4_917 0.50 +0.1 1,042,885 - Pf1N1B4_917 0.52 -0.3 1,043,001 + Pf1N1B4_917 0.63 +0.1 1,043,001 + Pf1N1B4_917 0.63 -1.8 1,043,004 + Pf1N1B4_917 0.63 +0.3 1,043,004 + Pf1N1B4_917 0.63 +0.1 1,043,004 + Pf1N1B4_917 0.63 +0.8 1,043,005 - Pf1N1B4_917 0.64 +0.1 1,043,005 - +1.1 1,043,005 - Pf1N1B4_917 0.64 -0.7 1,043,005 - Pf1N1B4_917 0.64 +0.1 1,043,255 + Pf1N1B4_917 0.88 -0.5 1,043,255 + Pf1N1B4_917 0.88 -0.0 1,043,256 - Pf1N1B4_917 0.88 -1.3 1,043,256 - Pf1N1B4_917 0.88 +1.7 1,043,469 - +1.0 1,043,483 + -0.3 1,043,483 + -0.5 1,043,484 - +0.5 1,043,484 - +0.1 1,043,504 + -0.3 1,043,505 - +1.1 1,043,505 - +0.8 1,043,505 - +2.9 1,043,505 - -0.9 1,043,508 - +0.3 1,043,508 - +0.9 1,043,530 + +0.1 1,043,530 + +0.1 1,043,531 - +0.8 1,043,531 - -0.2 1,043,565 - -0.5 1,043,565 - +0.6 1,043,646 + -1.5 1,043,646 + +0.1 1,043,647 - +0.4 1,043,647 - -1.5 1,043,647 - +1.0 1,043,647 - -0.9 1,043,647 - +2.1 1,043,647 - +0.8 1,043,647 - +1.0 1,043,647 - +2.7 1,043,647 - +0.8 1,043,647 - +1.8 1,043,654 + -0.3 1,043,654 + +0.1 1,043,655 - +0.3 1,043,655 - +1.5 1,043,655 - +1.1 1,043,655 - +1.2 1,043,655 - -2.7 1,043,655 - +0.3 1,043,655 - +1.2 1,043,670 + +1.1 1,043,671 - +0.8 1,043,671 - +1.7 1,043,671 - +0.6 1,043,671 - +3.8 1,043,671 - +1.2 1,043,780 + +1.7 1,043,781 - +0.9 1,043,794 - +0.9 1,043,794 - -0.3 1,043,794 - -0.4 1,043,817 + -1.1 1,043,818 - +1.3 1,043,818 - +0.4 1,043,818 - +1.1 1,043,818 - +0.1 1,043,993 - Pf1N1B4_918 0.22 +1.7 1,044,051 + Pf1N1B4_918 0.31 +0.3 1,044,058 + Pf1N1B4_918 0.32 +0.1 1,044,059 - Pf1N1B4_918 0.32 +0.4 1,044,059 - Pf1N1B4_918 0.32 +0.7 1,044,059 - Pf1N1B4_918 0.32 +1.1 1,044,059 - Pf1N1B4_918 0.32 +2.1 1,044,158 - Pf1N1B4_918 0.47 +0.1 1,044,158 - Pf1N1B4_918 0.47 +2.8 1,044,208 + Pf1N1B4_918 0.54 -0.7 1,044,209 - Pf1N1B4_918 0.55 -1.2 1,044,209 - Pf1N1B4_918 0.55 +0.1 1,044,253 + Pf1N1B4_918 0.61 -0.9 1,044,253 + Pf1N1B4_918 0.61 +1.7 1,044,254 - Pf1N1B4_918 0.61 +0.7 1,044,254 - Pf1N1B4_918 0.61 +1.4 1,044,254 - Pf1N1B4_918 0.61 +0.1 1,044,254 - Pf1N1B4_918 0.61 +0.7 1,044,270 + Pf1N1B4_918 0.64 -0.5 1,044,270 + Pf1N1B4_918 0.64 +0.6 1,044,271 - Pf1N1B4_918 0.64 +0.7 1,044,271 - Pf1N1B4_918 0.64 -0.2 1,044,348 + -1.5 1,044,348 + Pf1N1B4_918 0.76 +0.5 1,044,349 - Pf1N1B4_918 0.76 +0.9
Or see this region's nucleotide sequence