Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_742 and Pf1N1B4_743 are separated by 129 nucleotides
Pf1N1B4_742 - Argininosuccinate synthase (EC 6.3.4.5), at 844,308 to 845,525
_742
Pf1N1B4_743 - Sodium-type flagellar protein motY precursor, at 845,655 to 846,563
_743
Position (kb) 844
845
846 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 843.383 kb on - strand at 843.383 kb on - strand at 843.437 kb on + strand at 843.437 kb on + strand at 843.460 kb on - strand at 843.595 kb on - strand at 843.673 kb on + strand at 843.674 kb on - strand at 843.703 kb on + strand at 843.726 kb on + strand at 843.726 kb on + strand at 843.742 kb on + strand at 843.742 kb on + strand at 843.792 kb on - strand at 843.792 kb on - strand at 843.823 kb on - strand at 843.869 kb on + strand at 843.869 kb on + strand at 843.870 kb on - strand at 843.919 kb on + strand at 843.994 kb on + strand at 844.132 kb on - strand at 844.132 kb on - strand at 844.135 kb on + strand at 844.232 kb on + strand at 844.233 kb on - strand at 844.234 kb on + strand at 844.234 kb on + strand at 844.234 kb on + strand at 844.234 kb on + strand at 844.234 kb on + strand at 844.234 kb on + strand at 844.234 kb on + strand at 844.234 kb on + strand at 844.235 kb on - strand at 844.235 kb on - strand at 844.394 kb on - strand at 844.394 kb on - strand at 844.394 kb on - strand at 844.483 kb on + strand, within Pf1N1B4_742 at 844.483 kb on + strand, within Pf1N1B4_742 at 844.483 kb on + strand, within Pf1N1B4_742 at 844.484 kb on - strand, within Pf1N1B4_742 at 844.501 kb on - strand, within Pf1N1B4_742 at 844.579 kb on + strand, within Pf1N1B4_742 at 844.579 kb on + strand, within Pf1N1B4_742 at 844.580 kb on - strand, within Pf1N1B4_742 at 844.580 kb on - strand, within Pf1N1B4_742 at 844.594 kb on + strand, within Pf1N1B4_742 at 844.594 kb on + strand, within Pf1N1B4_742 at 844.595 kb on - strand, within Pf1N1B4_742 at 844.595 kb on - strand, within Pf1N1B4_742 at 844.595 kb on - strand, within Pf1N1B4_742 at 844.598 kb on + strand, within Pf1N1B4_742 at 844.599 kb on - strand, within Pf1N1B4_742 at 844.599 kb on - strand, within Pf1N1B4_742 at 844.599 kb on - strand, within Pf1N1B4_742 at 844.599 kb on - strand, within Pf1N1B4_742 at 844.599 kb on - strand, within Pf1N1B4_742 at 844.726 kb on + strand at 844.727 kb on - strand, within Pf1N1B4_742 at 844.775 kb on + strand, within Pf1N1B4_742 at 844.849 kb on + strand, within Pf1N1B4_742 at 844.849 kb on + strand, within Pf1N1B4_742 at 844.850 kb on - strand, within Pf1N1B4_742 at 844.850 kb on - strand, within Pf1N1B4_742 at 844.864 kb on + strand, within Pf1N1B4_742 at 844.865 kb on - strand, within Pf1N1B4_742 at 844.865 kb on - strand, within Pf1N1B4_742 at 844.865 kb on - strand, within Pf1N1B4_742 at 844.960 kb on + strand, within Pf1N1B4_742 at 844.960 kb on + strand, within Pf1N1B4_742 at 844.960 kb on + strand, within Pf1N1B4_742 at 844.960 kb on + strand, within Pf1N1B4_742 at 844.961 kb on - strand, within Pf1N1B4_742 at 844.961 kb on - strand, within Pf1N1B4_742 at 844.961 kb on - strand, within Pf1N1B4_742 at 844.961 kb on - strand, within Pf1N1B4_742 at 845.047 kb on + strand, within Pf1N1B4_742 at 845.126 kb on - strand, within Pf1N1B4_742 at 845.126 kb on - strand, within Pf1N1B4_742 at 845.252 kb on + strand, within Pf1N1B4_742 at 845.253 kb on - strand, within Pf1N1B4_742 at 845.253 kb on - strand, within Pf1N1B4_742 at 845.253 kb on - strand, within Pf1N1B4_742 at 845.264 kb on - strand, within Pf1N1B4_742 at 845.275 kb on + strand, within Pf1N1B4_742 at 845.276 kb on - strand, within Pf1N1B4_742 at 845.276 kb on - strand at 845.344 kb on + strand, within Pf1N1B4_742 at 845.345 kb on - strand, within Pf1N1B4_742 at 845.345 kb on - strand at 845.430 kb on + strand at 845.431 kb on - strand at 845.431 kb on - strand at 845.431 kb on - strand at 845.431 kb on - strand at 845.431 kb on - strand at 845.431 kb on - strand at 845.431 kb on - strand at 845.441 kb on + strand at 845.441 kb on + strand at 845.441 kb on + strand at 845.441 kb on + strand at 845.442 kb on - strand at 845.442 kb on - strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.443 kb on + strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.444 kb on - strand at 845.447 kb on + strand at 845.447 kb on + strand at 845.447 kb on + strand at 845.448 kb on - strand at 845.494 kb on + strand at 845.494 kb on + strand at 845.514 kb on + strand at 845.514 kb on + strand at 845.514 kb on + strand at 845.515 kb on - strand at 845.515 kb on - strand at 845.581 kb on + strand at 845.586 kb on + strand at 845.604 kb on + strand at 845.605 kb on - strand at 845.625 kb on + strand at 845.625 kb on + strand at 845.883 kb on + strand, within Pf1N1B4_743 at 845.883 kb on + strand, within Pf1N1B4_743 at 845.883 kb on + strand, within Pf1N1B4_743 at 845.883 kb on + strand, within Pf1N1B4_743 at 845.884 kb on - strand, within Pf1N1B4_743 at 845.884 kb on - strand, within Pf1N1B4_743 at 845.884 kb on - strand, within Pf1N1B4_743 at 845.884 kb on - strand, within Pf1N1B4_743 at 846.098 kb on - strand, within Pf1N1B4_743 at 846.106 kb on + strand, within Pf1N1B4_743 at 846.106 kb on + strand, within Pf1N1B4_743 at 846.106 kb on + strand, within Pf1N1B4_743 at 846.106 kb on + strand, within Pf1N1B4_743 at 846.106 kb on + strand, within Pf1N1B4_743 at 846.107 kb on - strand, within Pf1N1B4_743 at 846.107 kb on - strand, within Pf1N1B4_743 at 846.107 kb on - strand, within Pf1N1B4_743 at 846.107 kb on - strand, within Pf1N1B4_743 at 846.199 kb on - strand, within Pf1N1B4_743
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 843,383 - -1.2 843,383 - -1.0 843,437 + +0.0 843,437 + +0.0 843,460 - -0.1 843,595 - -0.6 843,673 + +1.9 843,674 - +1.0 843,703 + -1.0 843,726 + +0.0 843,726 + -0.3 843,742 + +1.0 843,742 + -1.0 843,792 - +0.0 843,792 - -1.6 843,823 - +0.6 843,869 + +0.0 843,869 + +0.6 843,870 - +0.0 843,919 + -0.6 843,994 + +1.5 844,132 - +1.0 844,132 - +0.0 844,135 + -0.7 844,232 + -1.6 844,233 - +1.0 844,234 + -0.2 844,234 + -0.2 844,234 + +1.0 844,234 + +2.3 844,234 + -1.0 844,234 + +1.6 844,234 + +0.2 844,234 + -2.0 844,235 - +0.0 844,235 - +0.0 844,394 - -2.0 844,394 - -1.6 844,394 - -2.3 844,483 + Pf1N1B4_742 0.14 -2.0 844,483 + Pf1N1B4_742 0.14 -3.5 844,483 + Pf1N1B4_742 0.14 -1.0 844,484 - Pf1N1B4_742 0.14 -4.9 844,501 - Pf1N1B4_742 0.16 -0.6 844,579 + Pf1N1B4_742 0.22 -1.6 844,579 + Pf1N1B4_742 0.22 -2.0 844,580 - Pf1N1B4_742 0.22 -1.6 844,580 - Pf1N1B4_742 0.22 -1.6 844,594 + Pf1N1B4_742 0.23 +0.0 844,594 + Pf1N1B4_742 0.23 +0.0 844,595 - Pf1N1B4_742 0.24 -1.0 844,595 - Pf1N1B4_742 0.24 -3.0 844,595 - Pf1N1B4_742 0.24 -1.6 844,598 + Pf1N1B4_742 0.24 -1.0 844,599 - Pf1N1B4_742 0.24 -1.6 844,599 - Pf1N1B4_742 0.24 -1.0 844,599 - Pf1N1B4_742 0.24 -2.8 844,599 - Pf1N1B4_742 0.24 +0.0 844,599 - Pf1N1B4_742 0.24 -2.3 844,726 + +0.0 844,727 - Pf1N1B4_742 0.34 -2.0 844,775 + Pf1N1B4_742 0.38 -1.6 844,849 + Pf1N1B4_742 0.44 -5.0 844,849 + Pf1N1B4_742 0.44 -1.0 844,850 - Pf1N1B4_742 0.44 -3.6 844,850 - Pf1N1B4_742 0.44 -1.0 844,864 + Pf1N1B4_742 0.46 -2.6 844,865 - Pf1N1B4_742 0.46 -3.0 844,865 - Pf1N1B4_742 0.46 -2.0 844,865 - Pf1N1B4_742 0.46 -2.3 844,960 + Pf1N1B4_742 0.54 -2.0 844,960 + Pf1N1B4_742 0.54 +0.5 844,960 + Pf1N1B4_742 0.54 -0.6 844,960 + Pf1N1B4_742 0.54 -2.8 844,961 - Pf1N1B4_742 0.54 -2.0 844,961 - Pf1N1B4_742 0.54 -4.8 844,961 - Pf1N1B4_742 0.54 -1.0 844,961 - Pf1N1B4_742 0.54 -2.3 845,047 + Pf1N1B4_742 0.61 -1.6 845,126 - Pf1N1B4_742 0.67 -0.6 845,126 - Pf1N1B4_742 0.67 -0.6 845,252 + Pf1N1B4_742 0.78 -1.0 845,253 - Pf1N1B4_742 0.78 -2.0 845,253 - Pf1N1B4_742 0.78 -3.3 845,253 - Pf1N1B4_742 0.78 -1.0 845,264 - Pf1N1B4_742 0.78 +0.0 845,275 + Pf1N1B4_742 0.79 -1.0 845,276 - Pf1N1B4_742 0.79 -2.3 845,276 - +0.0 845,344 + Pf1N1B4_742 0.85 -1.6 845,345 - Pf1N1B4_742 0.85 -2.0 845,345 - +0.0 845,430 + +0.0 845,431 - +0.9 845,431 - -1.0 845,431 - -1.0 845,431 - -2.0 845,431 - -2.0 845,431 - -2.3 845,431 - +0.0 845,441 + -2.0 845,441 + +0.0 845,441 + -1.0 845,441 + -1.6 845,442 - -2.0 845,442 - -0.4 845,443 + -1.6 845,443 + -3.3 845,443 + -2.6 845,443 + -2.8 845,443 + +0.0 845,443 + -1.0 845,443 + -2.0 845,443 + -3.0 845,443 + -2.6 845,443 + -1.6 845,443 + -4.4 845,443 + -1.0 845,443 + +0.0 845,443 + -1.0 845,443 + +0.0 845,443 + -1.0 845,444 - -1.0 845,444 - -2.8 845,444 - -2.0 845,444 - -1.6 845,444 - -1.0 845,444 - -2.3 845,444 - -2.0 845,444 - -2.3 845,444 - -2.0 845,444 - -1.0 845,444 - +0.0 845,447 + -2.0 845,447 + -2.0 845,447 + -2.3 845,448 - -1.6 845,494 + -2.3 845,494 + +0.0 845,514 + -1.6 845,514 + -2.8 845,514 + +0.0 845,515 - +0.0 845,515 - -2.3 845,581 + -3.9 845,586 + -1.6 845,604 + +2.0 845,605 - +0.3 845,625 + +1.0 845,625 + +0.0 845,883 + Pf1N1B4_743 0.25 +1.7 845,883 + Pf1N1B4_743 0.25 +0.0 845,883 + Pf1N1B4_743 0.25 -1.3 845,883 + Pf1N1B4_743 0.25 +0.0 845,884 - Pf1N1B4_743 0.25 +0.0 845,884 - Pf1N1B4_743 0.25 -1.6 845,884 - Pf1N1B4_743 0.25 +0.0 845,884 - Pf1N1B4_743 0.25 +0.0 846,098 - Pf1N1B4_743 0.49 -1.8 846,106 + Pf1N1B4_743 0.50 +1.3 846,106 + Pf1N1B4_743 0.50 +0.0 846,106 + Pf1N1B4_743 0.50 -0.4 846,106 + Pf1N1B4_743 0.50 +0.7 846,106 + Pf1N1B4_743 0.50 -1.4 846,107 - Pf1N1B4_743 0.50 -0.6 846,107 - Pf1N1B4_743 0.50 -0.6 846,107 - Pf1N1B4_743 0.50 +0.0 846,107 - Pf1N1B4_743 0.50 +0.0 846,199 - Pf1N1B4_743 0.60 -1.0
Or see this region's nucleotide sequence