Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5462 and Pf1N1B4_5463 are separated by 281 nucleotides Pf1N1B4_5463 and Pf1N1B4_5464 are separated by 325 nucleotides Pf1N1B4_5464 and Pf1N1B4_5465 are separated by 7 nucleotides
Pf1N1B4_5462 - hypothetical protein, at 209,255 to 210,247
_5462
Pf1N1B4_5463 - hypothetical protein, at 210,529 to 210,654
_5463
Pf1N1B4_5464 - Cointegrate resolution protein T, at 210,980 to 212,032
_5464
Pf1N1B4_5465 - hypothetical protein, at 212,040 to 213,158
_5465
Position (kb) 210
211
212
213 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 209.996 kb on + strand, within Pf1N1B4_5462 at 209.996 kb on + strand at 209.996 kb on + strand, within Pf1N1B4_5462 at 209.997 kb on - strand, within Pf1N1B4_5462 at 209.997 kb on - strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.000 kb on + strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.001 kb on - strand, within Pf1N1B4_5462 at 210.004 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.041 kb on + strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.042 kb on - strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.078 kb on + strand, within Pf1N1B4_5462 at 210.079 kb on - strand, within Pf1N1B4_5462 at 210.079 kb on - strand at 210.133 kb on + strand, within Pf1N1B4_5462 at 210.136 kb on + strand, within Pf1N1B4_5462 at 210.136 kb on + strand, within Pf1N1B4_5462 at 210.137 kb on - strand, within Pf1N1B4_5462 at 210.141 kb on + strand, within Pf1N1B4_5462 at 210.146 kb on + strand, within Pf1N1B4_5462 at 210.146 kb on + strand at 210.228 kb on + strand at 210.229 kb on - strand at 210.243 kb on + strand at 210.245 kb on + strand at 210.245 kb on + strand at 210.245 kb on + strand at 210.250 kb on + strand at 210.250 kb on + strand at 210.250 kb on + strand at 210.261 kb on + strand at 210.303 kb on - strand at 210.313 kb on - strand at 210.313 kb on - strand at 210.313 kb on - strand at 210.315 kb on + strand at 210.316 kb on - strand at 210.316 kb on - strand at 210.330 kb on - strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.344 kb on - strand at 210.408 kb on - strand at 210.408 kb on - strand at 210.408 kb on - strand at 210.435 kb on + strand at 210.436 kb on - strand at 210.552 kb on - strand, within Pf1N1B4_5463 at 210.557 kb on + strand at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.566 kb on - strand, within Pf1N1B4_5463 at 210.690 kb on + strand at 210.759 kb on - strand at 210.760 kb on + strand at 210.761 kb on - strand at 210.761 kb on - strand at 210.761 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.790 kb on - strand at 210.822 kb on + strand at 210.823 kb on - strand at 210.826 kb on + strand at 210.826 kb on + strand at 210.827 kb on - strand at 211.342 kb on - strand, within Pf1N1B4_5464 at 211.342 kb on - strand, within Pf1N1B4_5464 at 211.354 kb on - strand, within Pf1N1B4_5464 at 211.373 kb on + strand, within Pf1N1B4_5464 at 211.458 kb on + strand, within Pf1N1B4_5464 at 211.458 kb on + strand, within Pf1N1B4_5464 at 211.809 kb on + strand, within Pf1N1B4_5464 at 211.809 kb on + strand, within Pf1N1B4_5464 at 211.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 209,996 + Pf1N1B4_5462 0.75 +1.8 209,996 + -0.5 209,996 + Pf1N1B4_5462 0.75 +0.2 209,997 - Pf1N1B4_5462 0.75 -1.9 209,997 - Pf1N1B4_5462 0.75 +0.7 210,000 + Pf1N1B4_5462 0.75 +1.0 210,000 + Pf1N1B4_5462 0.75 -0.5 210,000 + Pf1N1B4_5462 0.75 +0.1 210,000 + Pf1N1B4_5462 0.75 -0.2 210,000 + -0.9 210,000 + Pf1N1B4_5462 0.75 +1.1 210,000 + Pf1N1B4_5462 0.75 -0.9 210,001 - Pf1N1B4_5462 0.75 +0.4 210,001 - Pf1N1B4_5462 0.75 -2.9 210,001 - Pf1N1B4_5462 0.75 +2.9 210,001 - Pf1N1B4_5462 0.75 +1.7 210,004 + Pf1N1B4_5462 0.75 -0.9 210,041 + Pf1N1B4_5462 0.79 +0.7 210,041 + Pf1N1B4_5462 0.79 -1.1 210,041 + Pf1N1B4_5462 0.79 +0.1 210,041 + Pf1N1B4_5462 0.79 +2.4 210,041 + Pf1N1B4_5462 0.79 +0.1 210,042 - Pf1N1B4_5462 0.79 +0.4 210,042 - Pf1N1B4_5462 0.79 +0.2 210,042 - Pf1N1B4_5462 0.79 -0.2 210,042 - Pf1N1B4_5462 0.79 -0.9 210,078 + Pf1N1B4_5462 0.83 +1.9 210,078 + Pf1N1B4_5462 0.83 +0.5 210,078 + Pf1N1B4_5462 0.83 +1.2 210,078 + Pf1N1B4_5462 0.83 +1.1 210,079 - Pf1N1B4_5462 0.83 +0.1 210,079 - -1.5 210,133 + Pf1N1B4_5462 0.88 +0.7 210,136 + Pf1N1B4_5462 0.89 +0.7 210,136 + Pf1N1B4_5462 0.89 +0.3 210,137 - Pf1N1B4_5462 0.89 -0.9 210,141 + Pf1N1B4_5462 0.89 -1.5 210,146 + Pf1N1B4_5462 0.90 -0.2 210,146 + -0.9 210,228 + +0.8 210,229 - +0.1 210,243 + +2.1 210,245 + -1.5 210,245 + +0.5 210,245 + +0.1 210,250 + +1.1 210,250 + +0.5 210,250 + +0.7 210,261 + +0.7 210,303 - +0.9 210,313 - +1.2 210,313 - +0.4 210,313 - -0.3 210,315 + +1.3 210,316 - -0.3 210,316 - +0.8 210,330 - +0.9 210,343 + -1.9 210,343 + +0.1 210,343 + -0.3 210,343 + -1.5 210,343 + +0.1 210,344 - +1.1 210,344 - +0.7 210,344 - +0.1 210,344 - -0.9 210,344 - +1.7 210,344 - -1.9 210,344 - -0.9 210,344 - +0.1 210,344 - +0.7 210,408 - +0.1 210,408 - +1.1 210,408 - -0.2 210,435 + +1.7 210,436 - -1.5 210,552 - Pf1N1B4_5463 0.18 -0.2 210,557 + +1.7 210,566 - Pf1N1B4_5463 0.29 +0.7 210,566 - Pf1N1B4_5463 0.29 +1.9 210,566 - Pf1N1B4_5463 0.29 +0.8 210,566 - Pf1N1B4_5463 0.29 -0.7 210,566 - Pf1N1B4_5463 0.29 -0.5 210,690 + -0.9 210,759 - -1.1 210,760 + +0.4 210,761 - -1.2 210,761 - +0.1 210,761 - +0.4 210,790 - +0.1 210,790 - -0.9 210,790 - +0.1 210,790 - +0.3 210,790 - +1.2 210,822 + +1.1 210,823 - +1.1 210,826 + +0.7 210,826 + +1.3 210,827 - +1.1 211,342 - Pf1N1B4_5464 0.34 -2.5 211,342 - Pf1N1B4_5464 0.34 -0.9 211,354 - Pf1N1B4_5464 0.36 -0.2 211,373 + Pf1N1B4_5464 0.37 +1.4 211,458 + Pf1N1B4_5464 0.45 -1.7 211,458 + Pf1N1B4_5464 0.45 +1.7 211,809 + Pf1N1B4_5464 0.79 -0.5 211,809 + Pf1N1B4_5464 0.79 +1.1 211,943 - -0.7
Or see this region's nucleotide sequence