Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4037 and Pf1N1B4_4038 overlap by 4 nucleotides Pf1N1B4_4038 and Pf1N1B4_4039 are separated by 2 nucleotides
Pf1N1B4_4037 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 4,488,385 to 4,489,740
_4037
Pf1N1B4_4038 - NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3), at 4,489,737 to 4,490,234
_4038
Pf1N1B4_4039 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 4,490,237 to 4,492,021
_4039
Position (kb)
4489
4490
4491 Strain fitness (log2 ratio)
-2
-1
0
1 at 4488.782 kb on + strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037 at 4489.185 kb on - strand at 4489.310 kb on + strand, within Pf1N1B4_4037 at 4489.741 kb on - strand at 4489.741 kb on - strand at 4489.741 kb on - strand at 4489.745 kb on - strand at 4490.011 kb on + strand, within Pf1N1B4_4038 at 4490.011 kb on + strand, within Pf1N1B4_4038 at 4490.012 kb on - strand, within Pf1N1B4_4038 at 4490.273 kb on - strand at 4490.354 kb on - strand at 4490.404 kb on - strand at 4490.404 kb on - strand at 4490.563 kb on - strand, within Pf1N1B4_4039 at 4490.950 kb on - strand, within Pf1N1B4_4039 at 4490.998 kb on - strand, within Pf1N1B4_4039 at 4491.060 kb on + strand, within Pf1N1B4_4039 at 4491.060 kb on + strand, within Pf1N1B4_4039 at 4491.060 kb on + strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 4,488,782 + Pf1N1B4_4037 0.29 -0.3 4,488,783 - Pf1N1B4_4037 0.29 -1.3 4,488,783 - Pf1N1B4_4037 0.29 -1.5 4,489,185 - -0.3 4,489,310 + Pf1N1B4_4037 0.68 -0.3 4,489,741 - -1.3 4,489,741 - +1.3 4,489,741 - +0.5 4,489,745 - +0.1 4,490,011 + Pf1N1B4_4038 0.55 +0.3 4,490,011 + Pf1N1B4_4038 0.55 +0.0 4,490,012 - Pf1N1B4_4038 0.55 -1.0 4,490,273 - -0.3 4,490,354 - -0.3 4,490,404 - -1.9 4,490,404 - -0.5 4,490,563 - Pf1N1B4_4039 0.18 +1.7 4,490,950 - Pf1N1B4_4039 0.40 -0.3 4,490,998 - Pf1N1B4_4039 0.43 +0.4 4,491,060 + Pf1N1B4_4039 0.46 +1.5 4,491,060 + Pf1N1B4_4039 0.46 -0.3 4,491,060 + Pf1N1B4_4039 0.46 -0.8 4,491,061 - Pf1N1B4_4039 0.46 +0.1 4,491,061 - Pf1N1B4_4039 0.46 +0.7 4,491,061 - Pf1N1B4_4039 0.46 -0.6 4,491,061 - Pf1N1B4_4039 0.46 +0.7
Or see this region's nucleotide sequence