Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2966 and Pf1N1B4_2967 are separated by 145 nucleotides Pf1N1B4_2967 and Pf1N1B4_2968 are separated by 24 nucleotides Pf1N1B4_2968 and Pf1N1B4_2969 are separated by 90 nucleotides
Pf1N1B4_2966 - FIG00958649: hypothetical protein, at 3,327,726 to 3,328,160
_2966
Pf1N1B4_2967 - Alpha/beta hydrolase, at 3,328,306 to 3,328,938
_2967
Pf1N1B4_2968 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 3,328,963 to 3,330,318
_2968
Pf1N1B4_2969 - ATPase, AFG1 family, at 3,330,409 to 3,331,503
_2969
Position (kb)
3328
3329
3330
3331 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3328.009 kb on + strand, within Pf1N1B4_2966 at 3328.174 kb on - strand at 3328.174 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.216 kb on + strand at 3328.217 kb on - strand at 3328.217 kb on - strand at 3328.219 kb on - strand at 3328.238 kb on - strand at 3328.238 kb on - strand at 3328.260 kb on + strand at 3328.323 kb on - strand at 3328.323 kb on - strand at 3328.360 kb on + strand at 3328.360 kb on + strand at 3328.361 kb on - strand at 3328.488 kb on + strand, within Pf1N1B4_2967 at 3328.489 kb on - strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.816 kb on + strand, within Pf1N1B4_2967 at 3328.816 kb on + strand at 3330.371 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.419 kb on + strand at 3330.419 kb on + strand at 3330.419 kb on + strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.506 kb on - strand at 3330.506 kb on - strand at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 3,328,009 + Pf1N1B4_2966 0.65 +0.1 3,328,174 - -1.2 3,328,174 - -0.1 3,328,191 - -0.0 3,328,191 - +1.7 3,328,191 - +0.1 3,328,191 - +0.1 3,328,191 - -0.3 3,328,191 - -0.9 3,328,216 + -0.9 3,328,217 - +1.7 3,328,217 - -1.0 3,328,219 - +0.1 3,328,238 - -2.0 3,328,238 - -0.3 3,328,260 + +1.3 3,328,323 - +0.4 3,328,323 - -0.4 3,328,360 + +0.1 3,328,360 + -2.2 3,328,361 - +0.1 3,328,488 + Pf1N1B4_2967 0.29 +0.3 3,328,489 - Pf1N1B4_2967 0.29 +0.1 3,328,761 + Pf1N1B4_2967 0.72 -0.4 3,328,761 + Pf1N1B4_2967 0.72 +0.1 3,328,761 + Pf1N1B4_2967 0.72 +1.1 3,328,761 + Pf1N1B4_2967 0.72 -0.2 3,328,761 + Pf1N1B4_2967 0.72 +0.7 3,328,761 + Pf1N1B4_2967 0.72 +0.1 3,328,761 + Pf1N1B4_2967 0.72 -0.1 3,328,761 + Pf1N1B4_2967 0.72 -2.2 3,328,762 - Pf1N1B4_2967 0.72 +0.9 3,328,762 - Pf1N1B4_2967 0.72 +1.5 3,328,762 - Pf1N1B4_2967 0.72 +0.8 3,328,762 - Pf1N1B4_2967 0.72 +0.1 3,328,762 - Pf1N1B4_2967 0.72 +1.3 3,328,762 - Pf1N1B4_2967 0.72 -0.7 3,328,762 - Pf1N1B4_2967 0.72 +1.4 3,328,762 - Pf1N1B4_2967 0.72 +1.5 3,328,762 - Pf1N1B4_2967 0.72 +1.1 3,328,762 - Pf1N1B4_2967 0.72 +0.1 3,328,762 - Pf1N1B4_2967 0.72 +0.7 3,328,816 + Pf1N1B4_2967 0.81 +0.9 3,328,816 + +0.1 3,330,371 - +0.6 3,330,409 - +0.7 3,330,409 - +0.1 3,330,409 - -1.2 3,330,409 - +0.1 3,330,409 - +2.5 3,330,409 - -2.0 3,330,409 - +1.7 3,330,419 + -0.6 3,330,419 + +0.4 3,330,419 + -0.9 3,330,420 - -1.3 3,330,420 - -0.5 3,330,420 - -1.2 3,330,420 - -1.3 3,330,420 - +0.6 3,330,420 - +1.1 3,330,505 + -0.6 3,330,505 + +0.4 3,330,505 + +1.0 3,330,505 + -0.5 3,330,505 + +0.6 3,330,506 - -0.9 3,330,506 - -1.7 3,330,595 + Pf1N1B4_2969 0.17 -0.3 3,330,595 + Pf1N1B4_2969 0.17 -0.5 3,330,595 + Pf1N1B4_2969 0.17 +1.4 3,330,595 + Pf1N1B4_2969 0.17 +0.1 3,330,595 + Pf1N1B4_2969 0.17 +0.1 3,330,595 + Pf1N1B4_2969 0.17 +0.4 3,330,595 + Pf1N1B4_2969 0.17 +0.8 3,330,595 + Pf1N1B4_2969 0.17 -1.7 3,330,596 - Pf1N1B4_2969 0.17 -0.4 3,330,596 - Pf1N1B4_2969 0.17 +0.9 3,330,596 - Pf1N1B4_2969 0.17 +0.6 3,330,596 - Pf1N1B4_2969 0.17 -1.2 3,330,596 - Pf1N1B4_2969 0.17 -1.9 3,330,596 - Pf1N1B4_2969 0.17 +0.8 3,330,596 - Pf1N1B4_2969 0.17 +0.7 3,330,596 - Pf1N1B4_2969 0.17 -0.4 3,330,596 - Pf1N1B4_2969 0.17 +0.1 3,330,596 - Pf1N1B4_2969 0.17 +0.4 3,330,596 - Pf1N1B4_2969 0.17 -0.9 3,330,596 - Pf1N1B4_2969 0.17 -0.3 3,330,628 + Pf1N1B4_2969 0.20 -0.1 3,330,628 + Pf1N1B4_2969 0.20 -1.6 3,330,628 + Pf1N1B4_2969 0.20 -0.9 3,330,628 + Pf1N1B4_2969 0.20 -1.4 3,330,628 + Pf1N1B4_2969 0.20 -1.6 3,330,628 + Pf1N1B4_2969 0.20 -0.9 3,330,628 + Pf1N1B4_2969 0.20 -1.1 3,330,628 + Pf1N1B4_2969 0.20 +1.1 3,330,628 + Pf1N1B4_2969 0.20 +0.1 3,330,628 + Pf1N1B4_2969 0.20 -1.9 3,330,628 + Pf1N1B4_2969 0.20 -0.4 3,330,628 + Pf1N1B4_2969 0.20 +0.1 3,330,628 + Pf1N1B4_2969 0.20 -0.2 3,330,628 + Pf1N1B4_2969 0.20 -0.9 3,330,629 - Pf1N1B4_2969 0.20 -1.2 3,330,629 - Pf1N1B4_2969 0.20 +0.1 3,330,629 - Pf1N1B4_2969 0.20 -1.1 3,330,629 - Pf1N1B4_2969 0.20 -0.3 3,330,629 - Pf1N1B4_2969 0.20 -0.9 3,330,629 - Pf1N1B4_2969 0.20 +0.8 3,330,629 - Pf1N1B4_2969 0.20 +1.1 3,330,629 - Pf1N1B4_2969 0.20 -0.4 3,330,629 - Pf1N1B4_2969 0.20 +0.1 3,330,629 - Pf1N1B4_2969 0.20 -0.9 3,330,629 - Pf1N1B4_2969 0.20 +0.9 3,330,629 - Pf1N1B4_2969 0.20 +1.7 3,330,629 - Pf1N1B4_2969 0.20 -0.4 3,330,629 - Pf1N1B4_2969 0.20 +0.9 3,330,629 - Pf1N1B4_2969 0.20 -0.9 3,330,629 - Pf1N1B4_2969 0.20 -1.4 3,330,629 - Pf1N1B4_2969 0.20 -2.4 3,330,916 + Pf1N1B4_2969 0.46 +0.8 3,330,916 + Pf1N1B4_2969 0.46 -0.9 3,330,916 + Pf1N1B4_2969 0.46 +1.4 3,330,916 + Pf1N1B4_2969 0.46 +0.1 3,330,916 + Pf1N1B4_2969 0.46 +0.8 3,330,916 + Pf1N1B4_2969 0.46 +0.1 3,330,916 + -1.4 3,330,916 + Pf1N1B4_2969 0.46 -0.9 3,330,916 + Pf1N1B4_2969 0.46 -0.2 3,330,916 + Pf1N1B4_2969 0.46 +0.1 3,330,916 + Pf1N1B4_2969 0.46 +1.1 3,330,917 - Pf1N1B4_2969 0.46 +0.1 3,330,917 - Pf1N1B4_2969 0.46 -1.1 3,330,917 - Pf1N1B4_2969 0.46 -0.6 3,330,917 - Pf1N1B4_2969 0.46 -0.3 3,330,917 - Pf1N1B4_2969 0.46 +1.9 3,330,917 - Pf1N1B4_2969 0.46 +0.1 3,330,917 - Pf1N1B4_2969 0.46 -0.4 3,331,136 + Pf1N1B4_2969 0.66 +0.9 3,331,136 + Pf1N1B4_2969 0.66 -0.9 3,331,136 + Pf1N1B4_2969 0.66 -1.9 3,331,136 + Pf1N1B4_2969 0.66 +1.1 3,331,136 + Pf1N1B4_2969 0.66 -0.5 3,331,136 + Pf1N1B4_2969 0.66 +0.9 3,331,136 + Pf1N1B4_2969 0.66 +0.6 3,331,136 + Pf1N1B4_2969 0.66 +0.1 3,331,136 + Pf1N1B4_2969 0.66 -0.1 3,331,136 + Pf1N1B4_2969 0.66 -1.1 3,331,136 + Pf1N1B4_2969 0.66 -0.4 3,331,136 + Pf1N1B4_2969 0.66 +0.6 3,331,136 + Pf1N1B4_2969 0.66 +1.9 3,331,136 + Pf1N1B4_2969 0.66 +0.1 3,331,136 + Pf1N1B4_2969 0.66 -0.1 3,331,136 + Pf1N1B4_2969 0.66 +0.1 3,331,136 + Pf1N1B4_2969 0.66 +1.9 3,331,136 + Pf1N1B4_2969 0.66 -0.9 3,331,136 + Pf1N1B4_2969 0.66 +1.7 3,331,136 + Pf1N1B4_2969 0.66 -0.7 3,331,136 + -1.4 3,331,136 + Pf1N1B4_2969 0.66 +0.7 3,331,136 + Pf1N1B4_2969 0.66 -2.0 3,331,137 - Pf1N1B4_2969 0.66 +0.6 3,331,137 - Pf1N1B4_2969 0.66 -0.8 3,331,137 - Pf1N1B4_2969 0.66 -1.9 3,331,137 - Pf1N1B4_2969 0.66 +0.9 3,331,137 - Pf1N1B4_2969 0.66 +0.1 3,331,137 - Pf1N1B4_2969 0.66 -0.4 3,331,137 - Pf1N1B4_2969 0.66 +1.7 3,331,137 - Pf1N1B4_2969 0.66 +0.5 3,331,137 - Pf1N1B4_2969 0.66 -1.2 3,331,137 - Pf1N1B4_2969 0.66 +0.9 3,331,137 - Pf1N1B4_2969 0.66 -0.1 3,331,137 - Pf1N1B4_2969 0.66 +0.4 3,331,240 + Pf1N1B4_2969 0.76 +0.9 3,331,240 + Pf1N1B4_2969 0.76 +0.9 3,331,240 + -0.9 3,331,240 + Pf1N1B4_2969 0.76 +0.5 3,331,240 + Pf1N1B4_2969 0.76 +0.1 3,331,240 + Pf1N1B4_2969 0.76 -2.2 3,331,240 + Pf1N1B4_2969 0.76 -0.1 3,331,240 + Pf1N1B4_2969 0.76 +0.1 3,331,240 + Pf1N1B4_2969 0.76 +1.1 3,331,240 + Pf1N1B4_2969 0.76 +0.7 3,331,241 - Pf1N1B4_2969 0.76 -0.9 3,331,241 - Pf1N1B4_2969 0.76 +1.5 3,331,241 - Pf1N1B4_2969 0.76 -1.2 3,331,241 - Pf1N1B4_2969 0.76 +0.7 3,331,241 - Pf1N1B4_2969 0.76 +0.6 3,331,241 - Pf1N1B4_2969 0.76 +0.1 3,331,241 - Pf1N1B4_2969 0.76 +1.1
Or see this region's nucleotide sequence