Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2335

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2334 and Pf1N1B4_2335 overlap by 4 nucleotidesPf1N1B4_2335 and Pf1N1B4_2336 overlap by 4 nucleotidesPf1N1B4_2336 and Pf1N1B4_2337 are separated by 26 nucleotides Pf1N1B4_2334 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 2,651,846 to 2,653,051 _2334 Pf1N1B4_2335 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 2,653,048 to 2,653,644 _2335 Pf1N1B4_2336 - FIG003551: hypothetical protein, at 2,653,641 to 2,654,075 _2336 Pf1N1B4_2337 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,102 to 2,654,722 _2337 Position (kb) 2653 2654Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2652.117 kb on + strand, within Pf1N1B4_2334at 2652.118 kb on - strand, within Pf1N1B4_2334at 2652.235 kb on + strand, within Pf1N1B4_2334at 2652.279 kb on + strandat 2652.502 kb on + strand, within Pf1N1B4_2334at 2652.822 kb on + strand, within Pf1N1B4_2334at 2652.870 kb on + strand, within Pf1N1B4_2334at 2652.870 kb on + strand, within Pf1N1B4_2334at 2652.871 kb on - strand, within Pf1N1B4_2334at 2652.921 kb on + strand, within Pf1N1B4_2334at 2652.922 kb on - strand, within Pf1N1B4_2334at 2652.957 kb on + strandat 2652.957 kb on + strandat 2652.957 kb on + strandat 2652.958 kb on - strandat 2652.958 kb on - strandat 2652.958 kb on - strandat 2653.125 kb on + strand, within Pf1N1B4_2335at 2653.125 kb on + strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.389 kb on - strand, within Pf1N1B4_2335at 2653.389 kb on - strand, within Pf1N1B4_2335at 2653.389 kb on - strand, within Pf1N1B4_2335at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strandat 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.757 kb on - strand, within Pf1N1B4_2336at 2653.757 kb on - strand, within Pf1N1B4_2336at 2653.759 kb on + strand, within Pf1N1B4_2336at 2653.856 kb on + strandat 2653.857 kb on - strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.964 kb on - strand, within Pf1N1B4_2336at 2653.964 kb on - strand, within Pf1N1B4_2336at 2654.048 kb on + strandat 2654.049 kb on - strandat 2654.049 kb on - strandat 2654.052 kb on + strandat 2654.052 kb on + strandat 2654.053 kb on - strandat 2654.064 kb on + strandat 2654.064 kb on + strandat 2654.165 kb on + strand, within Pf1N1B4_2337at 2654.174 kb on - strand, within Pf1N1B4_2337at 2654.180 kb on + strand, within Pf1N1B4_2337at 2654.180 kb on + strand, within Pf1N1B4_2337at 2654.180 kb on + strand, within Pf1N1B4_2337at 2654.180 kb on + strand, within Pf1N1B4_2337at 2654.181 kb on - strand, within Pf1N1B4_2337at 2654.181 kb on - strand, within Pf1N1B4_2337at 2654.307 kb on + strand, within Pf1N1B4_2337at 2654.307 kb on + strand, within Pf1N1B4_2337at 2654.307 kb on + strand, within Pf1N1B4_2337at 2654.308 kb on - strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strandat 2654.317 kb on - strand, within Pf1N1B4_2337at 2654.317 kb on - strand, within Pf1N1B4_2337at 2654.451 kb on + strand, within Pf1N1B4_2337

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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2,652,117 + Pf1N1B4_2334 0.22 +0.3
2,652,118 - Pf1N1B4_2334 0.23 -1.2
2,652,235 + Pf1N1B4_2334 0.32 +0.3
2,652,279 + +0.8
2,652,502 + Pf1N1B4_2334 0.54 +0.2
2,652,822 + Pf1N1B4_2334 0.81 -0.7
2,652,870 + Pf1N1B4_2334 0.85 -1.5
2,652,870 + Pf1N1B4_2334 0.85 -0.7
2,652,871 - Pf1N1B4_2334 0.85 -2.0
2,652,921 + Pf1N1B4_2334 0.89 +0.3
2,652,922 - Pf1N1B4_2334 0.89 +2.5
2,652,957 + +0.9
2,652,957 + +0.3
2,652,957 + +1.3
2,652,958 - +1.3
2,652,958 - -1.0
2,652,958 - +2.3
2,653,125 + Pf1N1B4_2335 0.13 -0.2
2,653,125 + Pf1N1B4_2335 0.13 -0.0
2,653,126 - Pf1N1B4_2335 0.13 +1.1
2,653,126 - Pf1N1B4_2335 0.13 -0.2
2,653,126 - Pf1N1B4_2335 0.13 -0.2
2,653,126 - Pf1N1B4_2335 0.13 -0.4
2,653,389 - Pf1N1B4_2335 0.57 -1.7
2,653,389 - Pf1N1B4_2335 0.57 -0.5
2,653,389 - Pf1N1B4_2335 0.57 -0.2
2,653,756 + Pf1N1B4_2336 0.26 -0.7
2,653,756 + Pf1N1B4_2336 0.26 +1.6
2,653,756 + Pf1N1B4_2336 0.26 +1.2
2,653,756 + +2.3
2,653,756 + Pf1N1B4_2336 0.26 +0.3
2,653,756 + Pf1N1B4_2336 0.26 -0.4
2,653,756 + Pf1N1B4_2336 0.26 +0.9
2,653,757 - Pf1N1B4_2336 0.27 +1.3
2,653,757 - Pf1N1B4_2336 0.27 +0.8
2,653,759 + Pf1N1B4_2336 0.27 +0.3
2,653,856 + +2.7
2,653,857 - Pf1N1B4_2336 0.50 -0.4
2,653,963 + Pf1N1B4_2336 0.74 -0.7
2,653,963 + Pf1N1B4_2336 0.74 +0.3
2,653,963 + Pf1N1B4_2336 0.74 +0.5
2,653,963 + Pf1N1B4_2336 0.74 -0.4
2,653,963 + Pf1N1B4_2336 0.74 +0.8
2,653,963 + Pf1N1B4_2336 0.74 +0.7
2,653,964 - Pf1N1B4_2336 0.74 +0.6
2,653,964 - Pf1N1B4_2336 0.74 +1.3
2,654,048 + -0.2
2,654,049 - +1.5
2,654,049 - +2.3
2,654,052 + -0.1
2,654,052 + +1.3
2,654,053 - +1.0
2,654,064 + -0.5
2,654,064 + -0.4
2,654,165 + Pf1N1B4_2337 0.10 -1.7
2,654,174 - Pf1N1B4_2337 0.12 -2.5
2,654,180 + Pf1N1B4_2337 0.13 -3.4
2,654,180 + Pf1N1B4_2337 0.13 -0.7
2,654,180 + Pf1N1B4_2337 0.13 +0.0
2,654,180 + Pf1N1B4_2337 0.13 -1.2
2,654,181 - Pf1N1B4_2337 0.13 -4.8
2,654,181 - Pf1N1B4_2337 0.13 -1.1
2,654,307 + Pf1N1B4_2337 0.33 -0.8
2,654,307 + Pf1N1B4_2337 0.33 +0.3
2,654,307 + Pf1N1B4_2337 0.33 -2.0
2,654,308 - Pf1N1B4_2337 0.33 -1.2
2,654,316 + Pf1N1B4_2337 0.34 -0.7
2,654,316 + Pf1N1B4_2337 0.34 +1.3
2,654,316 + Pf1N1B4_2337 0.34 +0.3
2,654,316 + Pf1N1B4_2337 0.34 -1.7
2,654,316 + Pf1N1B4_2337 0.34 -0.2
2,654,316 + Pf1N1B4_2337 0.34 -3.7
2,654,316 + -0.7
2,654,317 - Pf1N1B4_2337 0.35 -0.7
2,654,317 - Pf1N1B4_2337 0.35 -1.2
2,654,451 + Pf1N1B4_2337 0.56 -1.2

Or see this region's nucleotide sequence