Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_210 and Pf1N1B4_211 overlap by 1 nucleotides Pf1N1B4_211 and Pf1N1B4_212 overlap by 25 nucleotides Pf1N1B4_212 and Pf1N1B4_213 are separated by 122 nucleotides
Pf1N1B4_210 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 257,922 to 259,661
_210
Pf1N1B4_211 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 259,661 to 260,527
_211
Pf1N1B4_212 - Thiol:disulfide interchange protein DsbG precursor, at 260,503 to 261,270
_212
Pf1N1B4_213 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 261,393 to 262,205
_213
Position (kb) 259
260
261 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 258.855 kb on + strand at 258.855 kb on + strand, within Pf1N1B4_210 at 258.855 kb on + strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.856 kb on - strand, within Pf1N1B4_210 at 258.926 kb on - strand at 259.108 kb on - strand at 259.184 kb on - strand, within Pf1N1B4_210 at 259.251 kb on + strand, within Pf1N1B4_210 at 259.381 kb on - strand, within Pf1N1B4_210 at 259.384 kb on - strand, within Pf1N1B4_210 at 259.384 kb on - strand, within Pf1N1B4_210 at 259.465 kb on + strand at 259.466 kb on - strand, within Pf1N1B4_210 at 259.466 kb on - strand, within Pf1N1B4_210 at 259.466 kb on - strand, within Pf1N1B4_210 at 259.466 kb on - strand, within Pf1N1B4_210 at 259.466 kb on - strand, within Pf1N1B4_210 at 259.529 kb on + strand at 259.529 kb on + strand at 259.530 kb on - strand at 259.530 kb on - strand at 259.530 kb on - strand at 259.672 kb on + strand at 259.699 kb on + strand at 259.700 kb on - strand at 259.847 kb on + strand, within Pf1N1B4_211 at 259.848 kb on - strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.949 kb on + strand, within Pf1N1B4_211 at 259.950 kb on - strand, within Pf1N1B4_211 at 259.998 kb on + strand, within Pf1N1B4_211 at 259.998 kb on + strand, within Pf1N1B4_211 at 259.999 kb on - strand, within Pf1N1B4_211 at 260.044 kb on + strand, within Pf1N1B4_211 at 260.044 kb on + strand, within Pf1N1B4_211 at 260.045 kb on - strand, within Pf1N1B4_211 at 260.045 kb on - strand, within Pf1N1B4_211 at 260.045 kb on - strand, within Pf1N1B4_211 at 260.048 kb on + strand, within Pf1N1B4_211 at 260.048 kb on + strand, within Pf1N1B4_211 at 260.049 kb on - strand, within Pf1N1B4_211 at 260.049 kb on - strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.106 kb on + strand, within Pf1N1B4_211 at 260.107 kb on - strand, within Pf1N1B4_211 at 260.107 kb on - strand, within Pf1N1B4_211 at 260.107 kb on - strand, within Pf1N1B4_211 at 260.120 kb on + strand, within Pf1N1B4_211 at 260.120 kb on + strand, within Pf1N1B4_211 at 260.365 kb on + strand, within Pf1N1B4_211 at 260.366 kb on - strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.375 kb on + strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.376 kb on - strand, within Pf1N1B4_211 at 260.377 kb on + strand, within Pf1N1B4_211 at 260.675 kb on - strand, within Pf1N1B4_212 at 260.698 kb on + strand, within Pf1N1B4_212 at 260.698 kb on + strand, within Pf1N1B4_212 at 260.699 kb on - strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.723 kb on + strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.724 kb on - strand, within Pf1N1B4_212 at 260.792 kb on - strand, within Pf1N1B4_212 at 260.792 kb on - strand, within Pf1N1B4_212 at 260.878 kb on + strand, within Pf1N1B4_212 at 260.879 kb on - strand, within Pf1N1B4_212 at 260.906 kb on - strand, within Pf1N1B4_212 at 261.187 kb on + strand, within Pf1N1B4_212 at 261.188 kb on - strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.191 kb on + strand, within Pf1N1B4_212 at 261.192 kb on - strand, within Pf1N1B4_212 at 261.192 kb on - strand at 261.192 kb on - strand, within Pf1N1B4_212 at 261.192 kb on - strand, within Pf1N1B4_212 at 261.268 kb on + strand at 261.268 kb on + strand at 261.268 kb on + strand at 261.268 kb on + strand at 261.269 kb on - strand at 261.272 kb on + strand at 261.273 kb on - strand at 261.342 kb on + strand at 261.343 kb on - strand at 261.385 kb on - strand at 261.394 kb on + strand at 261.462 kb on - strand at 261.462 kb on - strand at 261.487 kb on + strand, within Pf1N1B4_213 at 261.488 kb on - strand, within Pf1N1B4_213
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 258,855 + +2.1 258,855 + Pf1N1B4_210 0.54 +0.5 258,855 + Pf1N1B4_210 0.54 +0.3 258,856 - Pf1N1B4_210 0.54 -1.6 258,856 - Pf1N1B4_210 0.54 +1.2 258,856 - Pf1N1B4_210 0.54 +1.0 258,856 - Pf1N1B4_210 0.54 +2.4 258,856 - Pf1N1B4_210 0.54 +0.6 258,926 - -1.8 259,108 - -0.2 259,184 - Pf1N1B4_210 0.73 +1.4 259,251 + Pf1N1B4_210 0.76 +0.4 259,381 - Pf1N1B4_210 0.84 +1.4 259,384 - Pf1N1B4_210 0.84 +0.5 259,384 - Pf1N1B4_210 0.84 +0.1 259,465 + +0.2 259,466 - Pf1N1B4_210 0.89 +1.6 259,466 - Pf1N1B4_210 0.89 +0.8 259,466 - Pf1N1B4_210 0.89 +1.7 259,466 - Pf1N1B4_210 0.89 -0.2 259,466 - Pf1N1B4_210 0.89 -0.1 259,529 + +1.4 259,529 + -0.2 259,530 - +0.1 259,530 - +1.7 259,530 - +1.4 259,672 + +1.7 259,699 + +1.4 259,700 - +1.0 259,847 + Pf1N1B4_211 0.21 +1.6 259,848 - Pf1N1B4_211 0.22 +1.3 259,949 + Pf1N1B4_211 0.33 +2.2 259,949 + Pf1N1B4_211 0.33 +3.4 259,949 + Pf1N1B4_211 0.33 +1.7 259,949 + Pf1N1B4_211 0.33 +3.7 259,949 + Pf1N1B4_211 0.33 +2.3 259,950 - Pf1N1B4_211 0.33 +1.2 259,998 + Pf1N1B4_211 0.39 +2.4 259,998 + Pf1N1B4_211 0.39 +1.6 259,999 - Pf1N1B4_211 0.39 +2.9 260,044 + Pf1N1B4_211 0.44 -0.2 260,044 + Pf1N1B4_211 0.44 +1.6 260,045 - Pf1N1B4_211 0.44 +2.2 260,045 - Pf1N1B4_211 0.44 +2.9 260,045 - Pf1N1B4_211 0.44 +1.3 260,048 + Pf1N1B4_211 0.45 +1.0 260,048 + Pf1N1B4_211 0.45 +3.4 260,049 - Pf1N1B4_211 0.45 +2.1 260,049 - Pf1N1B4_211 0.45 +1.2 260,106 + Pf1N1B4_211 0.51 +0.6 260,106 + Pf1N1B4_211 0.51 +0.5 260,106 + Pf1N1B4_211 0.51 +1.2 260,106 + Pf1N1B4_211 0.51 +2.7 260,106 + Pf1N1B4_211 0.51 +0.8 260,106 + Pf1N1B4_211 0.51 +1.1 260,106 + Pf1N1B4_211 0.51 +0.7 260,106 + Pf1N1B4_211 0.51 +2.2 260,107 - Pf1N1B4_211 0.51 +1.8 260,107 - Pf1N1B4_211 0.51 +0.8 260,107 - Pf1N1B4_211 0.51 +2.7 260,120 + Pf1N1B4_211 0.53 +1.4 260,120 + Pf1N1B4_211 0.53 +1.5 260,365 + Pf1N1B4_211 0.81 +1.1 260,366 - Pf1N1B4_211 0.81 +1.3 260,375 + Pf1N1B4_211 0.82 +1.4 260,375 + Pf1N1B4_211 0.82 +0.7 260,375 + Pf1N1B4_211 0.82 +0.5 260,375 + Pf1N1B4_211 0.82 +0.6 260,375 + Pf1N1B4_211 0.82 +0.6 260,375 + Pf1N1B4_211 0.82 +0.9 260,375 + Pf1N1B4_211 0.82 +2.0 260,375 + Pf1N1B4_211 0.82 +1.8 260,375 + Pf1N1B4_211 0.82 +1.8 260,375 + Pf1N1B4_211 0.82 +1.0 260,375 + Pf1N1B4_211 0.82 +1.5 260,375 + Pf1N1B4_211 0.82 +2.7 260,375 + Pf1N1B4_211 0.82 +0.2 260,375 + Pf1N1B4_211 0.82 +1.4 260,375 + Pf1N1B4_211 0.82 +2.5 260,375 + Pf1N1B4_211 0.82 +2.2 260,376 - Pf1N1B4_211 0.82 +0.4 260,376 - Pf1N1B4_211 0.82 +2.1 260,376 - Pf1N1B4_211 0.82 +1.5 260,376 - Pf1N1B4_211 0.82 +1.7 260,376 - Pf1N1B4_211 0.82 +1.2 260,376 - Pf1N1B4_211 0.82 +1.2 260,376 - Pf1N1B4_211 0.82 +2.1 260,376 - Pf1N1B4_211 0.82 +2.3 260,376 - Pf1N1B4_211 0.82 +0.4 260,376 - Pf1N1B4_211 0.82 +2.2 260,376 - Pf1N1B4_211 0.82 +1.4 260,376 - Pf1N1B4_211 0.82 +1.6 260,376 - Pf1N1B4_211 0.82 -0.7 260,376 - Pf1N1B4_211 0.82 +0.4 260,376 - Pf1N1B4_211 0.82 +1.6 260,376 - Pf1N1B4_211 0.82 +1.9 260,376 - Pf1N1B4_211 0.82 -0.2 260,377 + Pf1N1B4_211 0.83 +1.3 260,675 - Pf1N1B4_212 0.22 -0.9 260,698 + Pf1N1B4_212 0.25 +0.5 260,698 + Pf1N1B4_212 0.25 +0.2 260,699 - Pf1N1B4_212 0.26 +0.6 260,723 + Pf1N1B4_212 0.29 +0.4 260,723 + Pf1N1B4_212 0.29 +0.4 260,723 + Pf1N1B4_212 0.29 +0.4 260,723 + Pf1N1B4_212 0.29 +0.5 260,723 + Pf1N1B4_212 0.29 +0.3 260,724 - Pf1N1B4_212 0.29 -1.5 260,724 - Pf1N1B4_212 0.29 -1.3 260,724 - Pf1N1B4_212 0.29 -0.2 260,724 - Pf1N1B4_212 0.29 -2.2 260,724 - Pf1N1B4_212 0.29 -0.3 260,792 - Pf1N1B4_212 0.38 -0.2 260,792 - Pf1N1B4_212 0.38 -0.6 260,878 + Pf1N1B4_212 0.49 +1.5 260,879 - Pf1N1B4_212 0.49 +0.4 260,906 - Pf1N1B4_212 0.52 -1.2 261,187 + Pf1N1B4_212 0.89 +1.3 261,188 - Pf1N1B4_212 0.89 +1.1 261,191 + Pf1N1B4_212 0.90 +1.5 261,191 + Pf1N1B4_212 0.90 -0.2 261,191 + Pf1N1B4_212 0.90 +0.5 261,191 + Pf1N1B4_212 0.90 -0.6 261,191 + Pf1N1B4_212 0.90 +0.4 261,191 + Pf1N1B4_212 0.90 +0.4 261,191 + Pf1N1B4_212 0.90 +1.8 261,192 - Pf1N1B4_212 0.90 +0.5 261,192 - +0.5 261,192 - Pf1N1B4_212 0.90 +0.4 261,192 - Pf1N1B4_212 0.90 -0.8 261,268 + -0.2 261,268 + +0.4 261,268 + +0.9 261,268 + +1.1 261,269 - -0.1 261,272 + -1.8 261,273 - -1.2 261,342 + +0.9 261,343 - -0.2 261,385 - -0.2 261,394 + -2.2 261,462 - +1.4 261,462 - -0.9 261,487 + Pf1N1B4_213 0.12 -0.6 261,488 - Pf1N1B4_213 0.12 +0.6
Or see this region's nucleotide sequence