Experiment: Formate deepwell acetate and casamino FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05840 and MMP_RS05845 are separated by 186 nucleotides MMP_RS05845 and MMP_RS05850 are separated by 17 nucleotides MMP_RS05850 and MMP_RS05855 are separated by 122 nucleotides
MMP_RS05840: MMP_RS05840 - peptide chain release factor aRF-1, at 1,120,835 to 1,122,094
_RS05840
MMP_RS05845: MMP_RS05845 - tRNA-intron lyase, at 1,122,281 to 1,122,814
_RS05845
MMP_RS05850: MMP_RS05850 - Ldh family oxidoreductase, at 1,122,832 to 1,123,875
_RS05850
MMP_RS05855: MMP_RS05855 - FprA family A-type flavoprotein, at 1,123,998 to 1,125,221
_RS05855
Position (kb)
1122
1123
1124 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1122.094 kb on - strand at 1122.184 kb on - strand at 1122.184 kb on - strand at 1122.228 kb on + strand at 1122.229 kb on - strand at 1122.230 kb on + strand at 1122.233 kb on + strand at 1122.236 kb on - strand at 1122.236 kb on - strand at 1122.280 kb on - strand at 1122.280 kb on - strand at 1122.281 kb on + strand at 1122.466 kb on - strand, within MMP_RS05845 at 1122.747 kb on - strand, within MMP_RS05845 at 1122.760 kb on + strand, within MMP_RS05845 at 1122.760 kb on + strand, within MMP_RS05845 at 1123.047 kb on + strand, within MMP_RS05850 at 1123.173 kb on - strand, within MMP_RS05850 at 1123.437 kb on + strand, within MMP_RS05850 at 1123.764 kb on - strand, within MMP_RS05850 at 1123.765 kb on + strand, within MMP_RS05850 at 1123.808 kb on - strand at 1123.809 kb on + strand at 1123.809 kb on + strand at 1123.809 kb on + strand at 1123.809 kb on + strand at 1123.809 kb on + strand at 1123.812 kb on + strand at 1123.812 kb on + strand at 1123.816 kb on + strand at 1123.851 kb on - strand at 1123.854 kb on - strand at 1123.864 kb on - strand at 1123.904 kb on + strand at 1123.907 kb on + strand at 1123.933 kb on + strand at 1123.951 kb on + strand at 1123.951 kb on + strand at 1123.956 kb on + strand at 1123.961 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate deepwell acetate and casamino FREE remove 1,122,094 - -1.8 1,122,184 - -3.9 1,122,184 - -3.7 1,122,228 + -0.3 1,122,229 - -3.2 1,122,230 + -1.0 1,122,233 + -0.7 1,122,236 - -2.3 1,122,236 - -1.5 1,122,280 - -3.1 1,122,280 - -2.4 1,122,281 + -4.1 1,122,466 - MMP_RS05845 0.35 -2.3 1,122,747 - MMP_RS05845 0.87 -2.9 1,122,760 + MMP_RS05845 0.90 -0.1 1,122,760 + MMP_RS05845 0.90 -3.0 1,123,047 + MMP_RS05850 0.21 -3.4 1,123,173 - MMP_RS05850 0.33 -4.1 1,123,437 + MMP_RS05850 0.58 -2.3 1,123,764 - MMP_RS05850 0.89 -3.5 1,123,765 + MMP_RS05850 0.89 -2.8 1,123,808 - -4.2 1,123,809 + -4.0 1,123,809 + -2.4 1,123,809 + -3.5 1,123,809 + -5.5 1,123,809 + -4.0 1,123,812 + -3.3 1,123,812 + -3.7 1,123,816 + -3.0 1,123,851 - -3.1 1,123,854 - -4.6 1,123,864 - -3.7 1,123,904 + +0.2 1,123,907 + -0.1 1,123,933 + +0.4 1,123,951 + -0.0 1,123,951 + -0.1 1,123,956 + -0.0 1,123,961 - +0.7
Or see this region's nucleotide sequence