Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05265

Experiment: a-Cyclodextrin (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05255 and BWI76_RS05260 are separated by 130 nucleotidesBWI76_RS05260 and BWI76_RS05265 are separated by 3 nucleotidesBWI76_RS05265 and BWI76_RS05270 are separated by 102 nucleotidesBWI76_RS05270 and BWI76_RS05275 are separated by 3 nucleotides BWI76_RS05255: BWI76_RS05255 - outer membrane protein assembly factor BamA, at 1,105,725 to 1,108,154 _RS05255 BWI76_RS05260: BWI76_RS05260 - chaperone, at 1,108,285 to 1,108,770 _RS05260 BWI76_RS05265: BWI76_RS05265 - UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase, at 1,108,774 to 1,109,799 _RS05265 BWI76_RS05270: BWI76_RS05270 - beta-hydroxyacyl-ACP dehydratase, at 1,109,902 to 1,110,357 _RS05270 BWI76_RS05275: BWI76_RS05275 - acyl-[acyl-carrier-protein]--UDP-N- acetylglucosamine O-acyltransferase, at 1,110,361 to 1,111,149 _RS05275 Position (kb) 1108 1109 1110Strain fitness (log2 ratio) -1 0 1at 1108.659 kb on + strand, within BWI76_RS05260at 1109.794 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction a-Cyclodextrin (C)
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1,108,659 + BWI76_RS05260 0.77 -0.3
1,109,794 + -0.3

Or see this region's nucleotide sequence