Experiment: MoLS4 with limited (C) D,L-Lactate (7mM)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3349 and oorD are separated by 3 nucleotides oorD and queA are separated by 363 nucleotides
DVU3349: DVU3349 - pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-binding domain protein (TIGR), at 3,520,596 to 3,521,669
DVU3349
DVU3350: oorD - iron-sulfur cluster-binding protein (TIGR), at 3,521,673 to 3,521,906
oorD
DVU3351: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (TIGR), at 3,522,270 to 3,523,385
queA
Position (kb)
3521
3522 Strain fitness (log2 ratio)
-1
0
1 at 3520.932 kb on - strand, within DVU3349 at 3520.947 kb on - strand, within DVU3349 at 3521.403 kb on - strand, within DVU3349 at 3521.691 kb on - strand at 3521.716 kb on - strand, within oorD at 3521.745 kb on + strand, within oorD at 3521.851 kb on + strand, within oorD at 3521.854 kb on + strand, within oorD at 3521.854 kb on + strand, within oorD at 3521.859 kb on - strand, within oorD at 3522.051 kb on - strand at 3522.051 kb on - strand at 3522.120 kb on - strand at 3522.122 kb on - strand at 3522.122 kb on - strand at 3522.137 kb on + strand at 3522.137 kb on + strand at 3522.196 kb on + strand at 3522.209 kb on + strand at 3522.209 kb on + strand at 3522.217 kb on - strand at 3522.246 kb on + strand at 3522.254 kb on - strand at 3522.266 kb on + strand at 3522.319 kb on - strand at 3522.366 kb on + strand at 3522.367 kb on + strand at 3522.375 kb on - strand at 3522.400 kb on - strand, within queA at 3522.401 kb on + strand, within queA at 3522.439 kb on - strand, within queA at 3522.480 kb on + strand, within queA at 3522.488 kb on - strand, within queA at 3522.488 kb on - strand, within queA at 3522.490 kb on - strand, within queA at 3522.490 kb on - strand, within queA at 3522.492 kb on + strand, within queA at 3522.513 kb on + strand, within queA at 3522.530 kb on + strand, within queA at 3522.558 kb on + strand, within queA at 3522.558 kb on + strand, within queA at 3522.565 kb on + strand, within queA at 3522.565 kb on + strand, within queA at 3522.565 kb on + strand, within queA at 3522.592 kb on - strand, within queA at 3522.592 kb on - strand, within queA at 3522.621 kb on + strand, within queA at 3522.653 kb on + strand, within queA at 3522.671 kb on - strand, within queA at 3522.680 kb on + strand, within queA at 3522.680 kb on + strand, within queA at 3522.688 kb on - strand, within queA at 3522.692 kb on + strand, within queA at 3522.694 kb on - strand, within queA at 3522.786 kb on + strand, within queA at 3522.813 kb on - strand, within queA at 3522.822 kb on + strand, within queA at 3522.832 kb on - strand, within queA at 3522.887 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with limited (C) D,L-Lactate (7mM) remove 3,520,932 - DVU3349 0.31 +0.4 3,520,947 - DVU3349 0.33 +0.1 3,521,403 - DVU3349 0.75 +0.5 3,521,691 - +0.9 3,521,716 - oorD DVU3350 0.18 -1.6 3,521,745 + oorD DVU3350 0.31 -0.1 3,521,851 + oorD DVU3350 0.76 -0.4 3,521,854 + oorD DVU3350 0.77 -0.3 3,521,854 + oorD DVU3350 0.77 -0.1 3,521,859 - oorD DVU3350 0.79 +0.0 3,522,051 - -1.3 3,522,051 - +0.4 3,522,120 - +0.7 3,522,122 - -0.7 3,522,122 - -0.4 3,522,137 + +0.4 3,522,137 + +0.4 3,522,196 + -0.2 3,522,209 + -0.4 3,522,209 + -1.2 3,522,217 - -0.6 3,522,246 + -0.7 3,522,254 - -0.2 3,522,266 + -0.3 3,522,319 - +0.4 3,522,366 + -1.0 3,522,367 + -0.7 3,522,375 - -0.5 3,522,400 - queA DVU3351 0.12 +0.2 3,522,401 + queA DVU3351 0.12 -0.3 3,522,439 - queA DVU3351 0.15 -1.1 3,522,480 + queA DVU3351 0.19 -0.5 3,522,488 - queA DVU3351 0.20 +0.7 3,522,488 - queA DVU3351 0.20 +0.2 3,522,490 - queA DVU3351 0.20 +0.2 3,522,490 - queA DVU3351 0.20 -0.1 3,522,492 + queA DVU3351 0.20 +0.5 3,522,513 + queA DVU3351 0.22 +0.1 3,522,530 + queA DVU3351 0.23 +0.2 3,522,558 + queA DVU3351 0.26 +0.1 3,522,558 + queA DVU3351 0.26 -0.2 3,522,565 + queA DVU3351 0.26 +0.3 3,522,565 + queA DVU3351 0.26 +0.4 3,522,565 + queA DVU3351 0.26 +0.1 3,522,592 - queA DVU3351 0.29 -0.1 3,522,592 - queA DVU3351 0.29 +0.8 3,522,621 + queA DVU3351 0.31 -0.2 3,522,653 + queA DVU3351 0.34 -0.8 3,522,671 - queA DVU3351 0.36 +1.4 3,522,680 + queA DVU3351 0.37 -0.1 3,522,680 + queA DVU3351 0.37 +0.1 3,522,688 - queA DVU3351 0.37 -0.8 3,522,692 + queA DVU3351 0.38 +0.1 3,522,694 - queA DVU3351 0.38 +0.9 3,522,786 + queA DVU3351 0.46 -0.5 3,522,813 - queA DVU3351 0.49 +0.6 3,522,822 + queA DVU3351 0.49 +0.2 3,522,832 - queA DVU3351 0.50 +0.4 3,522,887 - queA DVU3351 0.55 -0.0
Or see this region's nucleotide sequence