Experiment: MoLS4 with limited (C) D,L-Lactate (7mM)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2090 and thiE-1 are separated by 134 nucleotides thiE-1 and moeB are separated by 268 nucleotides moeB and thiH overlap by 16 nucleotides
DVU2090: DVU2090 - EF hand domain protein (TIGR), at 2,179,568 to 2,180,404
DVU2090
DVU2091: thiE-1 - thiamine-phosphate pyrophosphorylase (TIGR), at 2,180,539 to 2,181,219
thiE-1
DVU2092: moeB - thiF family protein (TIGR), at 2,181,488 to 2,182,129
moeB
DVU2093: thiH - thiH protein (TIGR), at 2,182,114 to 2,183,448
thiH
Position (kb)
2180
2181
2182 Strain fitness (log2 ratio)
-1
0
1 at 2179.566 kb on - strand at 2179.586 kb on + strand at 2179.630 kb on - strand at 2179.635 kb on - strand at 2180.033 kb on + strand, within DVU2090 at 2180.068 kb on + strand, within DVU2090 at 2180.176 kb on - strand, within DVU2090 at 2180.198 kb on + strand, within DVU2090 at 2180.206 kb on - strand, within DVU2090 at 2180.227 kb on + strand, within DVU2090 at 2180.227 kb on + strand, within DVU2090 at 2180.263 kb on + strand, within DVU2090 at 2180.366 kb on + strand at 2180.440 kb on - strand at 2180.452 kb on + strand at 2180.467 kb on + strand at 2180.540 kb on + strand at 2180.600 kb on + strand at 2180.610 kb on + strand, within thiE-1 at 2180.687 kb on + strand, within thiE-1 at 2180.849 kb on + strand, within thiE-1 at 2180.857 kb on - strand, within thiE-1 at 2180.859 kb on + strand, within thiE-1 at 2180.919 kb on + strand, within thiE-1 at 2181.090 kb on + strand, within thiE-1 at 2181.093 kb on - strand, within thiE-1 at 2181.093 kb on - strand, within thiE-1 at 2181.108 kb on - strand, within thiE-1 at 2181.192 kb on + strand at 2181.236 kb on - strand at 2181.406 kb on + strand at 2181.432 kb on - strand at 2181.583 kb on + strand, within moeB at 2181.647 kb on - strand, within moeB at 2181.762 kb on + strand, within moeB at 2181.762 kb on + strand, within moeB at 2181.875 kb on - strand, within moeB at 2181.934 kb on - strand, within moeB at 2182.010 kb on - strand, within moeB at 2182.083 kb on - strand at 2182.139 kb on - strand at 2182.204 kb on - strand at 2182.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with limited (C) D,L-Lactate (7mM) remove 2,179,566 - +0.0 2,179,586 + +0.1 2,179,630 - -1.1 2,179,635 - +0.3 2,180,033 + DVU2090 0.56 +0.6 2,180,068 + DVU2090 0.60 +0.1 2,180,176 - DVU2090 0.73 +0.3 2,180,198 + DVU2090 0.75 +0.1 2,180,206 - DVU2090 0.76 -0.0 2,180,227 + DVU2090 0.79 -0.1 2,180,227 + DVU2090 0.79 +0.0 2,180,263 + DVU2090 0.83 -0.0 2,180,366 + +0.5 2,180,440 - -0.4 2,180,452 + -0.5 2,180,467 + -1.7 2,180,540 + -0.0 2,180,600 + -0.0 2,180,610 + thiE-1 DVU2091 0.10 -0.5 2,180,687 + thiE-1 DVU2091 0.22 -1.7 2,180,849 + thiE-1 DVU2091 0.46 -0.9 2,180,857 - thiE-1 DVU2091 0.47 -0.4 2,180,859 + thiE-1 DVU2091 0.47 -0.9 2,180,919 + thiE-1 DVU2091 0.56 -0.1 2,181,090 + thiE-1 DVU2091 0.81 -1.0 2,181,093 - thiE-1 DVU2091 0.81 -0.1 2,181,093 - thiE-1 DVU2091 0.81 +0.4 2,181,108 - thiE-1 DVU2091 0.84 -0.5 2,181,192 + -0.8 2,181,236 - +0.1 2,181,406 + +0.0 2,181,432 - -0.5 2,181,583 + moeB DVU2092 0.15 -0.9 2,181,647 - moeB DVU2092 0.25 -1.0 2,181,762 + moeB DVU2092 0.43 +0.0 2,181,762 + moeB DVU2092 0.43 -0.5 2,181,875 - moeB DVU2092 0.60 -0.7 2,181,934 - moeB DVU2092 0.69 -0.7 2,182,010 - moeB DVU2092 0.81 -0.3 2,182,083 - -0.3 2,182,139 - +0.0 2,182,204 - +0.2 2,182,219 - -0.2
Or see this region's nucleotide sequence