Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5992 and Pf1N1B4_5993 overlap by 4 nucleotides Pf1N1B4_5993 and Pf1N1B4_5994 are separated by 14 nucleotides Pf1N1B4_5994 and Pf1N1B4_5995 overlap by 4 nucleotides Pf1N1B4_5995 and Pf1N1B4_5996 are separated by 91 nucleotides
Pf1N1B4_5992 - Inner membrane protein, at 443,714 to 444,724
_5992
Pf1N1B4_5993 - Ferredoxin, 2Fe-2S, at 444,721 to 445,029
_5993
Pf1N1B4_5994 - 2-hydroxychromene-2-carboxylate isomerase/DsbA-like thioredoxin domain, at 445,044 to 445,706
_5994
Pf1N1B4_5995 - Transporter, MFS superfamily, at 445,703 to 447,034
_5995
Pf1N1B4_5996 - Transposase, at 447,126 to 448,178
_5996
Position (kb) 445
446
447
448 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 444.757 kb on + strand, within Pf1N1B4_5993 at 444.800 kb on - strand, within Pf1N1B4_5993 at 444.800 kb on - strand, within Pf1N1B4_5993 at 444.822 kb on - strand, within Pf1N1B4_5993 at 445.357 kb on - strand, within Pf1N1B4_5994 at 445.401 kb on - strand, within Pf1N1B4_5994 at 445.618 kb on - strand, within Pf1N1B4_5994 at 445.688 kb on + strand at 445.689 kb on - strand at 445.692 kb on + strand at 445.693 kb on - strand at 445.693 kb on - strand at 445.759 kb on + strand at 445.797 kb on + strand at 445.798 kb on - strand at 446.247 kb on + strand, within Pf1N1B4_5995 at 446.263 kb on - strand, within Pf1N1B4_5995 at 446.323 kb on - strand, within Pf1N1B4_5995 at 446.357 kb on - strand, within Pf1N1B4_5995 at 446.544 kb on - strand, within Pf1N1B4_5995 at 446.698 kb on - strand, within Pf1N1B4_5995 at 446.922 kb on + strand at 446.922 kb on + strand at 446.923 kb on - strand at 446.971 kb on - strand at 447.058 kb on + strand at 447.058 kb on + strand at 447.059 kb on - strand at 447.059 kb on - strand at 447.059 kb on - strand at 447.230 kb on - strand at 447.230 kb on - strand at 447.344 kb on + strand, within Pf1N1B4_5996 at 447.345 kb on - strand, within Pf1N1B4_5996 at 447.345 kb on - strand, within Pf1N1B4_5996 at 447.375 kb on - strand, within Pf1N1B4_5996 at 447.544 kb on + strand, within Pf1N1B4_5996 at 447.544 kb on + strand, within Pf1N1B4_5996 at 447.544 kb on + strand, within Pf1N1B4_5996 at 447.545 kb on - strand, within Pf1N1B4_5996 at 447.545 kb on - strand, within Pf1N1B4_5996 at 448.027 kb on + strand, within Pf1N1B4_5996
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 444,757 + Pf1N1B4_5993 0.12 +2.9 444,800 - Pf1N1B4_5993 0.26 -1.9 444,800 - Pf1N1B4_5993 0.26 -1.2 444,822 - Pf1N1B4_5993 0.33 +0.4 445,357 - Pf1N1B4_5994 0.47 +1.1 445,401 - Pf1N1B4_5994 0.54 -1.5 445,618 - Pf1N1B4_5994 0.87 -1.7 445,688 + +2.3 445,689 - -0.5 445,692 + -1.9 445,693 - -0.3 445,693 - -0.5 445,759 + +2.1 445,797 + -0.5 445,798 - +0.1 446,247 + Pf1N1B4_5995 0.41 +0.4 446,263 - Pf1N1B4_5995 0.42 +0.1 446,323 - Pf1N1B4_5995 0.47 +0.1 446,357 - Pf1N1B4_5995 0.49 -1.9 446,544 - Pf1N1B4_5995 0.63 -1.5 446,698 - Pf1N1B4_5995 0.75 -1.5 446,922 + +0.2 446,922 + +1.3 446,923 - +0.1 446,971 - +2.7 447,058 + -0.5 447,058 + -1.2 447,059 - -0.9 447,059 - +1.7 447,059 - +0.1 447,230 - +0.1 447,230 - +0.2 447,344 + Pf1N1B4_5996 0.21 -0.9 447,345 - Pf1N1B4_5996 0.21 +0.1 447,345 - Pf1N1B4_5996 0.21 +2.9 447,375 - Pf1N1B4_5996 0.24 -0.6 447,544 + Pf1N1B4_5996 0.40 -0.5 447,544 + Pf1N1B4_5996 0.40 -0.4 447,544 + Pf1N1B4_5996 0.40 -1.5 447,545 - Pf1N1B4_5996 0.40 -0.6 447,545 - Pf1N1B4_5996 0.40 -0.5 448,027 + Pf1N1B4_5996 0.86 -2.2
Or see this region's nucleotide sequence