Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5883 and Pf1N1B4_5884 are separated by 380 nucleotides Pf1N1B4_5884 and Pf1N1B4_5885 overlap by 4 nucleotides
Pf1N1B4_5883 - FIG00960892: hypothetical protein, at 325,237 to 326,934
_5883
Pf1N1B4_5884 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 327,315 to 328,667
_5884
Pf1N1B4_5885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 328,664 to 329,722
_5885
Position (kb) 327
328
329 Strain fitness (log2 ratio) -2
-1
0
1
2 at 326.393 kb on + strand, within Pf1N1B4_5883 at 326.394 kb on - strand at 326.426 kb on + strand, within Pf1N1B4_5883 at 326.426 kb on + strand, within Pf1N1B4_5883 at 326.426 kb on + strand, within Pf1N1B4_5883 at 326.427 kb on - strand, within Pf1N1B4_5883 at 326.676 kb on - strand at 326.676 kb on - strand, within Pf1N1B4_5883 at 326.836 kb on - strand at 326.883 kb on - strand at 326.897 kb on + strand at 326.898 kb on - strand at 327.068 kb on + strand at 327.286 kb on - strand at 327.328 kb on + strand at 327.342 kb on + strand at 327.355 kb on + strand at 327.356 kb on - strand at 327.879 kb on + strand, within Pf1N1B4_5884 at 327.880 kb on - strand at 328.121 kb on + strand, within Pf1N1B4_5884 at 328.266 kb on - strand, within Pf1N1B4_5884 at 328.381 kb on - strand, within Pf1N1B4_5884 at 328.417 kb on - strand, within Pf1N1B4_5884 at 328.597 kb on + strand at 328.597 kb on + strand at 328.598 kb on - strand at 328.671 kb on + strand at 328.671 kb on + strand at 328.744 kb on - strand at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.938 kb on - strand, within Pf1N1B4_5885 at 328.962 kb on - strand, within Pf1N1B4_5885 at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.036 kb on + strand at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.121 kb on - strand, within Pf1N1B4_5885 at 329.162 kb on + strand at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.481 kb on - strand, within Pf1N1B4_5885 at 329.660 kb on + strand at 329.661 kb on - strand at 329.663 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 326,393 + Pf1N1B4_5883 0.68 -0.9 326,394 - -0.9 326,426 + Pf1N1B4_5883 0.70 +0.8 326,426 + Pf1N1B4_5883 0.70 -1.9 326,426 + Pf1N1B4_5883 0.70 -0.8 326,427 - Pf1N1B4_5883 0.70 -2.5 326,676 - -0.9 326,676 - Pf1N1B4_5883 0.85 -0.3 326,836 - -1.9 326,883 - +0.7 326,897 + +1.7 326,898 - -2.2 327,068 + -0.9 327,286 - -0.9 327,328 + +0.0 327,342 + +1.7 327,355 + -0.3 327,356 - +0.7 327,879 + Pf1N1B4_5884 0.42 -0.9 327,880 - -0.9 328,121 + Pf1N1B4_5884 0.60 +0.1 328,266 - Pf1N1B4_5884 0.70 -0.9 328,381 - Pf1N1B4_5884 0.79 -0.5 328,417 - Pf1N1B4_5884 0.81 -0.9 328,597 + +1.4 328,597 + -0.2 328,598 - +1.1 328,671 + -0.9 328,671 + -0.2 328,744 - +0.1 328,773 - Pf1N1B4_5885 0.10 +1.1 328,773 - Pf1N1B4_5885 0.10 -0.9 328,938 - Pf1N1B4_5885 0.26 +2.4 328,962 - Pf1N1B4_5885 0.28 -0.9 329,036 + Pf1N1B4_5885 0.35 -0.1 329,036 + +0.1 329,036 + Pf1N1B4_5885 0.35 -1.5 329,121 - Pf1N1B4_5885 0.43 +0.1 329,162 + +0.1 329,162 + Pf1N1B4_5885 0.47 -0.8 329,162 + Pf1N1B4_5885 0.47 +0.5 329,163 - Pf1N1B4_5885 0.47 -0.5 329,163 - Pf1N1B4_5885 0.47 -0.1 329,324 + Pf1N1B4_5885 0.62 +0.7 329,324 + Pf1N1B4_5885 0.62 -0.9 329,325 - Pf1N1B4_5885 0.62 -0.0 329,325 - Pf1N1B4_5885 0.62 +1.4 329,442 - Pf1N1B4_5885 0.73 -0.5 329,442 - Pf1N1B4_5885 0.73 +0.8 329,481 - Pf1N1B4_5885 0.77 -1.5 329,660 + -0.9 329,661 - +0.1 329,663 + +1.1
Or see this region's nucleotide sequence