Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5863 and Pf1N1B4_5864 are separated by 1 nucleotides Pf1N1B4_5864 and Pf1N1B4_5865 are separated by 129 nucleotides
Pf1N1B4_5863 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 297,840 to 298,889
_5863
Pf1N1B4_5864 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 298,891 to 300,108
_5864
Pf1N1B4_5865 - FIG00960493: hypothetical protein, at 300,238 to 301,629
_5865
Position (kb) 298
299
300
301 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.306 kb on - strand, within Pf1N1B4_5863 at 298.554 kb on + strand at 298.583 kb on - strand, within Pf1N1B4_5863 at 298.583 kb on - strand, within Pf1N1B4_5863 at 298.887 kb on + strand at 298.887 kb on + strand at 298.888 kb on - strand at 298.890 kb on + strand at 298.891 kb on - strand at 299.002 kb on + strand at 299.003 kb on - strand at 299.047 kb on + strand, within Pf1N1B4_5864 at 299.047 kb on + strand, within Pf1N1B4_5864 at 299.048 kb on - strand, within Pf1N1B4_5864 at 299.531 kb on - strand, within Pf1N1B4_5864 at 299.692 kb on + strand, within Pf1N1B4_5864 at 299.693 kb on - strand, within Pf1N1B4_5864 at 299.814 kb on + strand at 299.849 kb on + strand, within Pf1N1B4_5864 at 299.929 kb on + strand at 300.060 kb on + strand at 300.061 kb on - strand at 300.328 kb on + strand at 300.532 kb on + strand, within Pf1N1B4_5865 at 300.533 kb on - strand, within Pf1N1B4_5865 at 300.766 kb on + strand at 300.767 kb on - strand, within Pf1N1B4_5865 at 300.909 kb on + strand, within Pf1N1B4_5865
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 298,003 - Pf1N1B4_5863 0.16 +0.7 298,003 - Pf1N1B4_5863 0.16 -0.4 298,306 - Pf1N1B4_5863 0.44 +0.3 298,554 + +1.1 298,583 - Pf1N1B4_5863 0.71 +0.8 298,583 - Pf1N1B4_5863 0.71 +0.3 298,887 + -0.9 298,887 + +3.1 298,888 - -0.9 298,890 + +0.1 298,891 - -2.5 299,002 + -1.9 299,003 - -0.9 299,047 + Pf1N1B4_5864 0.13 -1.9 299,047 + Pf1N1B4_5864 0.13 -0.9 299,048 - Pf1N1B4_5864 0.13 -0.9 299,531 - Pf1N1B4_5864 0.53 -1.2 299,692 + Pf1N1B4_5864 0.66 -1.5 299,693 - Pf1N1B4_5864 0.66 +1.1 299,814 + -0.9 299,849 + Pf1N1B4_5864 0.79 +2.1 299,929 + +0.1 300,060 + -1.5 300,061 - -0.6 300,328 + -1.5 300,532 + Pf1N1B4_5865 0.21 -0.2 300,533 - Pf1N1B4_5865 0.21 +0.2 300,766 + -1.9 300,767 - Pf1N1B4_5865 0.38 -0.5 300,909 + Pf1N1B4_5865 0.48 -0.9
Or see this region's nucleotide sequence