Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5621 and Pf1N1B4_5622 are separated by 629 nucleotides Pf1N1B4_5622 and Pf1N1B4_5623 are separated by 58 nucleotides
Pf1N1B4_5621 - Hydrolase, carbon-nitrogen family, at 47,070 to 47,828
_5621
Pf1N1B4_5622 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 48,458 to 49,546
_5622
Pf1N1B4_5623 - hypothetical protein, at 49,605 to 50,657
_5623
Position (kb)
48
49
50 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 47.766 kb on - strand at 47.891 kb on + strand at 47.958 kb on + strand at 47.958 kb on + strand at 48.084 kb on + strand at 48.084 kb on + strand at 48.140 kb on + strand at 48.140 kb on + strand at 48.262 kb on + strand at 48.262 kb on + strand at 48.271 kb on + strand at 48.271 kb on + strand at 48.272 kb on - strand at 48.272 kb on - strand at 48.272 kb on - strand at 48.307 kb on - strand at 48.559 kb on - strand at 48.761 kb on + strand, within Pf1N1B4_5622 at 48.807 kb on - strand, within Pf1N1B4_5622 at 49.205 kb on + strand at 49.206 kb on - strand, within Pf1N1B4_5622 at 49.621 kb on - strand at 49.621 kb on - strand at 49.727 kb on + strand, within Pf1N1B4_5623 at 49.728 kb on - strand, within Pf1N1B4_5623 at 49.732 kb on - strand, within Pf1N1B4_5623 at 49.732 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.006 kb on - strand, within Pf1N1B4_5623 at 50.043 kb on - strand, within Pf1N1B4_5623 at 50.298 kb on + strand, within Pf1N1B4_5623 at 50.339 kb on - strand at 50.348 kb on - strand, within Pf1N1B4_5623 at 50.348 kb on - strand at 50.389 kb on - strand, within Pf1N1B4_5623 at 50.389 kb on - strand, within Pf1N1B4_5623
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 47,766 - -0.7 47,891 + +0.1 47,958 + +0.4 47,958 + -0.9 48,084 + +0.1 48,084 + -0.5 48,140 + -1.5 48,140 + +0.1 48,262 + -0.2 48,262 + -2.5 48,271 + -0.9 48,271 + +0.3 48,272 - -0.9 48,272 - -1.5 48,272 - -1.9 48,307 - -0.3 48,559 - +1.1 48,761 + Pf1N1B4_5622 0.28 -1.8 48,807 - Pf1N1B4_5622 0.32 +0.7 49,205 + -1.9 49,206 - Pf1N1B4_5622 0.69 -0.9 49,621 - +0.8 49,621 - -1.5 49,727 + Pf1N1B4_5623 0.12 +1.1 49,728 - Pf1N1B4_5623 0.12 -1.5 49,732 - Pf1N1B4_5623 0.12 -1.2 49,732 - Pf1N1B4_5623 0.12 -1.9 50,006 - Pf1N1B4_5623 0.38 +0.2 50,006 - Pf1N1B4_5623 0.38 +0.1 50,006 - Pf1N1B4_5623 0.38 +2.4 50,043 - Pf1N1B4_5623 0.42 -2.9 50,298 + Pf1N1B4_5623 0.66 +2.1 50,339 - +1.7 50,348 - Pf1N1B4_5623 0.71 +1.4 50,348 - -1.5 50,389 - Pf1N1B4_5623 0.74 +0.3 50,389 - Pf1N1B4_5623 0.74 -0.9
Or see this region's nucleotide sequence