Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5425 and Pf1N1B4_5426 are separated by 461 nucleotides Pf1N1B4_5426 and Pf1N1B4_5427 are separated by 130 nucleotides Pf1N1B4_5427 and Pf1N1B4_5428 are separated by 474 nucleotides
Pf1N1B4_5425 - DNA-binding protein HU-beta, at 165,496 to 165,771
_5425
Pf1N1B4_5426 - hypothetical protein, at 166,233 to 166,580
_5426
Pf1N1B4_5427 - C4-type zinc finger protein, DksA/TraR family, at 166,711 to 167,184
_5427
Pf1N1B4_5428 - hypothetical protein, at 167,659 to 168,645
_5428
Position (kb) 166
167
168 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 165.724 kb on + strand, within Pf1N1B4_5425 at 165.725 kb on - strand, within Pf1N1B4_5425 at 165.828 kb on - strand at 165.848 kb on + strand at 165.849 kb on - strand at 165.903 kb on - strand at 165.949 kb on + strand at 165.950 kb on - strand at 165.950 kb on - strand at 166.031 kb on - strand at 166.031 kb on - strand at 166.031 kb on - strand at 166.128 kb on - strand at 166.211 kb on - strand at 166.222 kb on + strand at 166.222 kb on + strand at 166.223 kb on - strand at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.274 kb on + strand, within Pf1N1B4_5426 at 166.275 kb on - strand, within Pf1N1B4_5426 at 166.275 kb on - strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.287 kb on + strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.288 kb on - strand, within Pf1N1B4_5426 at 166.347 kb on - strand, within Pf1N1B4_5426 at 166.371 kb on + strand, within Pf1N1B4_5426 at 166.372 kb on - strand, within Pf1N1B4_5426 at 166.439 kb on - strand, within Pf1N1B4_5426 at 166.532 kb on - strand, within Pf1N1B4_5426 at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.603 kb on + strand at 166.604 kb on - strand at 166.604 kb on - strand at 166.604 kb on - strand at 166.819 kb on + strand, within Pf1N1B4_5427 at 166.819 kb on + strand, within Pf1N1B4_5427 at 166.820 kb on - strand, within Pf1N1B4_5427 at 166.820 kb on - strand, within Pf1N1B4_5427 at 166.893 kb on - strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.976 kb on + strand, within Pf1N1B4_5427 at 166.977 kb on - strand at 166.986 kb on + strand, within Pf1N1B4_5427 at 166.986 kb on + strand, within Pf1N1B4_5427 at 166.987 kb on - strand, within Pf1N1B4_5427 at 166.987 kb on - strand, within Pf1N1B4_5427 at 167.056 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.125 kb on + strand, within Pf1N1B4_5427 at 167.126 kb on - strand, within Pf1N1B4_5427 at 167.128 kb on - strand, within Pf1N1B4_5427 at 167.182 kb on + strand at 167.182 kb on + strand at 167.183 kb on - strand at 167.239 kb on - strand at 167.275 kb on - strand at 167.355 kb on - strand at 167.381 kb on - strand at 167.381 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.384 kb on - strand at 167.438 kb on - strand at 167.476 kb on - strand at 167.566 kb on - strand at 167.566 kb on - strand at 167.594 kb on + strand at 167.594 kb on + strand at 167.595 kb on - strand at 167.595 kb on - strand at 167.629 kb on + strand at 167.629 kb on + strand at 167.640 kb on - strand at 167.640 kb on - strand at 167.672 kb on - strand at 167.745 kb on + strand at 167.766 kb on + strand, within Pf1N1B4_5428 at 167.788 kb on + strand, within Pf1N1B4_5428 at 167.788 kb on + strand, within Pf1N1B4_5428 at 167.843 kb on - strand, within Pf1N1B4_5428 at 168.034 kb on + strand, within Pf1N1B4_5428 at 168.035 kb on - strand, within Pf1N1B4_5428 at 168.052 kb on + strand, within Pf1N1B4_5428 at 168.053 kb on - strand, within Pf1N1B4_5428 at 168.053 kb on - strand at 168.069 kb on + strand, within Pf1N1B4_5428 at 168.070 kb on - strand, within Pf1N1B4_5428 at 168.130 kb on + strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.131 kb on - strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.133 kb on + strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.134 kb on - strand, within Pf1N1B4_5428 at 168.145 kb on + strand, within Pf1N1B4_5428
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 165,724 + Pf1N1B4_5425 0.83 -1.1 165,725 - Pf1N1B4_5425 0.83 +2.5 165,828 - +0.1 165,848 + +1.1 165,849 - -0.5 165,903 - +0.5 165,949 + +2.1 165,950 - +0.5 165,950 - +0.5 166,031 - -0.5 166,031 - +0.9 166,031 - -0.8 166,128 - +1.1 166,211 - +1.3 166,222 + +0.9 166,222 + +1.5 166,223 - +1.1 166,274 + Pf1N1B4_5426 0.12 -1.5 166,274 + Pf1N1B4_5426 0.12 -0.7 166,274 + Pf1N1B4_5426 0.12 +1.8 166,275 - Pf1N1B4_5426 0.12 +1.8 166,275 - Pf1N1B4_5426 0.12 -0.5 166,287 + Pf1N1B4_5426 0.16 -0.8 166,287 + Pf1N1B4_5426 0.16 -0.9 166,287 + Pf1N1B4_5426 0.16 -1.1 166,287 + Pf1N1B4_5426 0.16 +2.1 166,287 + Pf1N1B4_5426 0.16 +1.4 166,288 - Pf1N1B4_5426 0.16 -0.2 166,288 - Pf1N1B4_5426 0.16 +0.5 166,288 - Pf1N1B4_5426 0.16 -0.8 166,347 - Pf1N1B4_5426 0.33 -0.7 166,371 + Pf1N1B4_5426 0.40 +1.5 166,372 - Pf1N1B4_5426 0.40 -1.1 166,439 - Pf1N1B4_5426 0.59 +2.3 166,532 - Pf1N1B4_5426 0.86 +0.5 166,603 + -1.1 166,603 + +1.5 166,603 + -0.5 166,603 + -1.1 166,603 + -0.1 166,604 - +0.5 166,604 - +1.1 166,604 - -0.5 166,819 + Pf1N1B4_5427 0.23 -0.1 166,819 + Pf1N1B4_5427 0.23 +0.2 166,820 - Pf1N1B4_5427 0.23 -2.1 166,820 - Pf1N1B4_5427 0.23 -1.8 166,893 - Pf1N1B4_5427 0.38 +0.5 166,976 + Pf1N1B4_5427 0.56 -1.1 166,976 + Pf1N1B4_5427 0.56 +0.5 166,976 + Pf1N1B4_5427 0.56 +1.1 166,976 + Pf1N1B4_5427 0.56 -1.1 166,976 + Pf1N1B4_5427 0.56 +0.9 166,976 + Pf1N1B4_5427 0.56 -1.1 166,976 + Pf1N1B4_5427 0.56 -1.3 166,976 + Pf1N1B4_5427 0.56 +2.1 166,976 + Pf1N1B4_5427 0.56 +0.5 166,977 - +0.5 166,986 + Pf1N1B4_5427 0.58 +0.9 166,986 + Pf1N1B4_5427 0.58 -1.1 166,987 - Pf1N1B4_5427 0.58 -1.1 166,987 - Pf1N1B4_5427 0.58 -0.5 167,056 + Pf1N1B4_5427 0.73 -1.8 167,125 + Pf1N1B4_5427 0.87 -1.1 167,125 + Pf1N1B4_5427 0.87 +0.8 167,125 + Pf1N1B4_5427 0.87 -1.6 167,125 + Pf1N1B4_5427 0.87 -1.5 167,125 + Pf1N1B4_5427 0.87 +1.1 167,125 + Pf1N1B4_5427 0.87 +0.9 167,125 + Pf1N1B4_5427 0.87 -0.5 167,126 - Pf1N1B4_5427 0.88 +0.5 167,128 - Pf1N1B4_5427 0.88 -0.5 167,182 + -0.5 167,182 + -0.5 167,183 - -0.0 167,239 - -0.5 167,275 - -0.6 167,355 - -0.5 167,381 - +1.4 167,381 - +2.5 167,384 - +0.5 167,384 - +0.5 167,384 - +0.6 167,384 - +1.5 167,384 - +0.3 167,384 - -1.1 167,438 - +1.9 167,476 - +0.3 167,566 - -0.2 167,566 - +1.9 167,594 + -1.1 167,594 + +0.9 167,595 - -0.5 167,595 - +0.5 167,629 + +0.4 167,629 + +1.3 167,640 - -1.3 167,640 - -0.5 167,672 - -1.1 167,745 + -0.5 167,766 + Pf1N1B4_5428 0.11 -0.1 167,788 + Pf1N1B4_5428 0.13 -1.8 167,788 + Pf1N1B4_5428 0.13 -1.1 167,843 - Pf1N1B4_5428 0.19 -3.4 168,034 + Pf1N1B4_5428 0.38 -0.5 168,035 - Pf1N1B4_5428 0.38 +0.5 168,052 + Pf1N1B4_5428 0.40 -0.6 168,053 - Pf1N1B4_5428 0.40 -1.1 168,053 - +0.5 168,069 + Pf1N1B4_5428 0.42 +1.1 168,070 - Pf1N1B4_5428 0.42 +0.3 168,130 + Pf1N1B4_5428 0.48 +0.8 168,131 - Pf1N1B4_5428 0.48 -1.1 168,131 - Pf1N1B4_5428 0.48 -0.2 168,131 - Pf1N1B4_5428 0.48 -0.5 168,131 - Pf1N1B4_5428 0.48 +1.2 168,133 + Pf1N1B4_5428 0.48 -1.8 168,133 + Pf1N1B4_5428 0.48 -0.8 168,133 + Pf1N1B4_5428 0.48 -0.5 168,133 + Pf1N1B4_5428 0.48 +0.5 168,133 + Pf1N1B4_5428 0.48 -1.5 168,133 + Pf1N1B4_5428 0.48 +0.5 168,134 - Pf1N1B4_5428 0.48 +0.5 168,134 - Pf1N1B4_5428 0.48 +3.1 168,145 + Pf1N1B4_5428 0.49 +1.5
Or see this region's nucleotide sequence