Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5082

Experiment: L-Methionine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5081 and Pf1N1B4_5082 are separated by 226 nucleotidesPf1N1B4_5082 and Pf1N1B4_5083 are separated by 24 nucleotidesPf1N1B4_5083 and Pf1N1B4_5084 are separated by 2 nucleotides Pf1N1B4_5081 - Transcriptional activator RfaH, at 5,630,326 to 5,630,847 _5081 Pf1N1B4_5082 - Capsular polysaccharide synthesis enzyme CpsB, at 5,631,074 to 5,632,228 _5082 Pf1N1B4_5083 - Capsular polysaccharide synthesis enzyme CpsC, polysaccharide export, at 5,632,253 to 5,632,774 _5083 Pf1N1B4_5084 - Capsular polysaccharide synthesis enzyme CpsD, exopolysaccharide synthesis, at 5,632,777 to 5,635,023 _5084 Position (kb) 5631 5632 5633Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5630.224 kb on - strandat 5630.244 kb on + strandat 5631.342 kb on - strand, within Pf1N1B4_5082at 5631.445 kb on + strand, within Pf1N1B4_5082at 5631.449 kb on + strand, within Pf1N1B4_5082at 5631.457 kb on + strand, within Pf1N1B4_5082at 5631.458 kb on - strand, within Pf1N1B4_5082at 5631.673 kb on + strandat 5631.674 kb on - strand, within Pf1N1B4_5082at 5631.812 kb on + strand, within Pf1N1B4_5082at 5631.900 kb on - strand, within Pf1N1B4_5082at 5632.089 kb on - strandat 5632.089 kb on - strand, within Pf1N1B4_5082at 5632.089 kb on - strand, within Pf1N1B4_5082at 5632.219 kb on - strandat 5632.342 kb on - strand, within Pf1N1B4_5083at 5632.342 kb on - strand, within Pf1N1B4_5083at 5632.554 kb on - strand, within Pf1N1B4_5083at 5632.773 kb on - strandat 5632.786 kb on - strandat 5632.799 kb on - strandat 5633.072 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Methionine (N)
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5,630,224 - +1.4
5,630,244 + -3.6
5,631,342 - Pf1N1B4_5082 0.23 -1.3
5,631,445 + Pf1N1B4_5082 0.32 -2.0
5,631,449 + Pf1N1B4_5082 0.32 +1.1
5,631,457 + Pf1N1B4_5082 0.33 +1.4
5,631,458 - Pf1N1B4_5082 0.33 -1.8
5,631,673 + -1.2
5,631,674 - Pf1N1B4_5082 0.52 -1.2
5,631,812 + Pf1N1B4_5082 0.64 -2.0
5,631,900 - Pf1N1B4_5082 0.72 -0.2
5,632,089 - +0.8
5,632,089 - Pf1N1B4_5082 0.88 -1.8
5,632,089 - Pf1N1B4_5082 0.88 +0.4
5,632,219 - -0.9
5,632,342 - Pf1N1B4_5083 0.17 -1.2
5,632,342 - Pf1N1B4_5083 0.17 +0.8
5,632,554 - Pf1N1B4_5083 0.58 -1.2
5,632,773 - -2.1
5,632,786 - -1.8
5,632,799 - +1.8
5,633,072 - -0.8

Or see this region's nucleotide sequence