Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5081 and Pf1N1B4_5082 are separated by 226 nucleotides Pf1N1B4_5082 and Pf1N1B4_5083 are separated by 24 nucleotides Pf1N1B4_5083 and Pf1N1B4_5084 are separated by 2 nucleotides
Pf1N1B4_5081 - Transcriptional activator RfaH, at 5,630,326 to 5,630,847
_5081
Pf1N1B4_5082 - Capsular polysaccharide synthesis enzyme CpsB, at 5,631,074 to 5,632,228
_5082
Pf1N1B4_5083 - Capsular polysaccharide synthesis enzyme CpsC, polysaccharide export, at 5,632,253 to 5,632,774
_5083
Pf1N1B4_5084 - Capsular polysaccharide synthesis enzyme CpsD, exopolysaccharide synthesis, at 5,632,777 to 5,635,023
_5084
Position (kb)
5631
5632
5633 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5630.224 kb on - strand at 5630.244 kb on + strand at 5631.342 kb on - strand, within Pf1N1B4_5082 at 5631.445 kb on + strand, within Pf1N1B4_5082 at 5631.449 kb on + strand, within Pf1N1B4_5082 at 5631.457 kb on + strand, within Pf1N1B4_5082 at 5631.458 kb on - strand, within Pf1N1B4_5082 at 5631.673 kb on + strand at 5631.674 kb on - strand, within Pf1N1B4_5082 at 5631.812 kb on + strand, within Pf1N1B4_5082 at 5631.900 kb on - strand, within Pf1N1B4_5082 at 5632.089 kb on - strand at 5632.089 kb on - strand, within Pf1N1B4_5082 at 5632.089 kb on - strand, within Pf1N1B4_5082 at 5632.219 kb on - strand at 5632.342 kb on - strand, within Pf1N1B4_5083 at 5632.342 kb on - strand, within Pf1N1B4_5083 at 5632.554 kb on - strand, within Pf1N1B4_5083 at 5632.773 kb on - strand at 5632.786 kb on - strand at 5632.799 kb on - strand at 5633.072 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 5,630,224 - +1.4 5,630,244 + -3.6 5,631,342 - Pf1N1B4_5082 0.23 -1.3 5,631,445 + Pf1N1B4_5082 0.32 -2.0 5,631,449 + Pf1N1B4_5082 0.32 +1.1 5,631,457 + Pf1N1B4_5082 0.33 +1.4 5,631,458 - Pf1N1B4_5082 0.33 -1.8 5,631,673 + -1.2 5,631,674 - Pf1N1B4_5082 0.52 -1.2 5,631,812 + Pf1N1B4_5082 0.64 -2.0 5,631,900 - Pf1N1B4_5082 0.72 -0.2 5,632,089 - +0.8 5,632,089 - Pf1N1B4_5082 0.88 -1.8 5,632,089 - Pf1N1B4_5082 0.88 +0.4 5,632,219 - -0.9 5,632,342 - Pf1N1B4_5083 0.17 -1.2 5,632,342 - Pf1N1B4_5083 0.17 +0.8 5,632,554 - Pf1N1B4_5083 0.58 -1.2 5,632,773 - -2.1 5,632,786 - -1.8 5,632,799 - +1.8 5,633,072 - -0.8
Or see this region's nucleotide sequence