Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4482 and Pf1N1B4_4483 are separated by 104 nucleotides Pf1N1B4_4483 and Pf1N1B4_4484 are separated by 183 nucleotides Pf1N1B4_4484 and Pf1N1B4_4485 are separated by 9 nucleotides
Pf1N1B4_4482 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 4,943,549 to 4,944,412
_4482
Pf1N1B4_4483 - Transcriptional regulator, ArsR family, at 4,944,517 to 4,944,852
_4483
Pf1N1B4_4484 - FIG002984: FAD-dependent pyridine nucleotide-disulphide oxidoreductase, at 4,945,036 to 4,946,280
_4484
Pf1N1B4_4485 - membrane protein, putative, at 4,946,290 to 4,947,096
_4485
Position (kb)
4945
4946
4947 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4944.303 kb on + strand, within Pf1N1B4_4482 at 4944.351 kb on + strand at 4944.352 kb on - strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.361 kb on - strand at 4944.366 kb on + strand at 4944.367 kb on - strand at 4944.367 kb on - strand at 4944.465 kb on + strand at 4944.465 kb on + strand at 4944.466 kb on - strand at 4944.735 kb on - strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.758 kb on - strand, within Pf1N1B4_4483 at 4944.758 kb on - strand at 4944.758 kb on - strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.762 kb on - strand, within Pf1N1B4_4483 at 4944.766 kb on + strand, within Pf1N1B4_4483 at 4944.766 kb on + strand, within Pf1N1B4_4483 at 4944.767 kb on - strand, within Pf1N1B4_4483 at 4944.820 kb on + strand at 4944.821 kb on - strand at 4944.977 kb on + strand at 4945.021 kb on - strand at 4945.083 kb on - strand at 4945.085 kb on + strand at 4945.086 kb on - strand at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.184 kb on - strand, within Pf1N1B4_4484 at 4945.220 kb on - strand, within Pf1N1B4_4484 at 4945.220 kb on - strand, within Pf1N1B4_4484 at 4945.447 kb on + strand, within Pf1N1B4_4484 at 4945.448 kb on - strand, within Pf1N1B4_4484 at 4945.448 kb on - strand, within Pf1N1B4_4484 at 4945.468 kb on + strand, within Pf1N1B4_4484 at 4945.469 kb on - strand, within Pf1N1B4_4484 at 4945.567 kb on + strand, within Pf1N1B4_4484 at 4945.568 kb on - strand, within Pf1N1B4_4484 at 4945.568 kb on - strand at 4945.568 kb on - strand, within Pf1N1B4_4484 at 4945.568 kb on - strand, within Pf1N1B4_4484 at 4945.685 kb on - strand, within Pf1N1B4_4484 at 4945.797 kb on - strand, within Pf1N1B4_4484 at 4945.927 kb on + strand, within Pf1N1B4_4484 at 4946.012 kb on + strand, within Pf1N1B4_4484 at 4946.062 kb on + strand, within Pf1N1B4_4484 at 4946.062 kb on + strand, within Pf1N1B4_4484 at 4946.289 kb on + strand at 4946.289 kb on + strand at 4946.289 kb on + strand at 4946.548 kb on + strand, within Pf1N1B4_4485 at 4946.548 kb on + strand, within Pf1N1B4_4485 at 4946.548 kb on + strand, within Pf1N1B4_4485 at 4947.194 kb on - strand at 4947.194 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 4,944,303 + Pf1N1B4_4482 0.87 -1.6 4,944,351 + -0.0 4,944,352 - -2.4 4,944,360 + -1.6 4,944,360 + -2.6 4,944,360 + -1.6 4,944,360 + -2.9 4,944,361 - -1.0 4,944,366 + -1.0 4,944,367 - -2.4 4,944,367 - -2.0 4,944,465 + -0.0 4,944,465 + +2.0 4,944,466 - -0.8 4,944,735 - Pf1N1B4_4483 0.65 -0.0 4,944,757 + Pf1N1B4_4483 0.71 +0.3 4,944,757 + Pf1N1B4_4483 0.71 +1.8 4,944,757 + Pf1N1B4_4483 0.71 +1.4 4,944,758 - Pf1N1B4_4483 0.72 -0.0 4,944,758 - -0.0 4,944,758 - Pf1N1B4_4483 0.72 -0.0 4,944,761 + Pf1N1B4_4483 0.73 +1.3 4,944,761 + Pf1N1B4_4483 0.73 +1.6 4,944,761 + Pf1N1B4_4483 0.73 +1.3 4,944,761 + Pf1N1B4_4483 0.73 +0.8 4,944,761 + Pf1N1B4_4483 0.73 +2.0 4,944,761 + Pf1N1B4_4483 0.73 +1.3 4,944,761 + Pf1N1B4_4483 0.73 +0.4 4,944,762 - Pf1N1B4_4483 0.73 -2.0 4,944,766 + Pf1N1B4_4483 0.74 +2.1 4,944,766 + Pf1N1B4_4483 0.74 +2.0 4,944,767 - Pf1N1B4_4483 0.74 -1.4 4,944,820 + +0.5 4,944,821 - +1.5 4,944,977 + +1.5 4,945,021 - -1.5 4,945,083 - -0.0 4,945,085 + -0.6 4,945,086 - -1.9 4,945,180 + Pf1N1B4_4484 0.12 -0.9 4,945,180 + Pf1N1B4_4484 0.12 -1.6 4,945,180 + Pf1N1B4_4484 0.12 -2.4 4,945,184 - Pf1N1B4_4484 0.12 -2.9 4,945,220 - Pf1N1B4_4484 0.15 -0.6 4,945,220 - Pf1N1B4_4484 0.15 -1.6 4,945,447 + Pf1N1B4_4484 0.33 -0.1 4,945,448 - Pf1N1B4_4484 0.33 -0.1 4,945,448 - Pf1N1B4_4484 0.33 -1.5 4,945,468 + Pf1N1B4_4484 0.35 -0.1 4,945,469 - Pf1N1B4_4484 0.35 -2.9 4,945,567 + Pf1N1B4_4484 0.43 -1.6 4,945,568 - Pf1N1B4_4484 0.43 -2.1 4,945,568 - -0.1 4,945,568 - Pf1N1B4_4484 0.43 -2.4 4,945,568 - Pf1N1B4_4484 0.43 -1.1 4,945,685 - Pf1N1B4_4484 0.52 -0.1 4,945,797 - Pf1N1B4_4484 0.61 -0.5 4,945,927 + Pf1N1B4_4484 0.72 -2.4 4,946,012 + Pf1N1B4_4484 0.78 +2.3 4,946,062 + Pf1N1B4_4484 0.82 -0.1 4,946,062 + Pf1N1B4_4484 0.82 -1.1 4,946,289 + -0.1 4,946,289 + -1.1 4,946,289 + -0.1 4,946,548 + Pf1N1B4_4485 0.32 -1.1 4,946,548 + Pf1N1B4_4485 0.32 +0.4 4,946,548 + Pf1N1B4_4485 0.32 -3.1 4,947,194 - -0.1 4,947,194 - +0.4
Or see this region's nucleotide sequence