Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4229 and Pf1N1B4_4230 are separated by 148 nucleotides
Pf1N1B4_4229 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 4,684,339 to 4,687,173
_4229
Pf1N1B4_4230 - Catalase (EC 1.11.1.6) / Peroxidase (EC 1.11.1.7), at 4,687,322 to 4,689,583
_4230
Position (kb)
4687
4688
4689
4690 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4686.596 kb on - strand, within Pf1N1B4_4229 at 4686.702 kb on - strand, within Pf1N1B4_4229 at 4686.752 kb on - strand, within Pf1N1B4_4229 at 4686.900 kb on + strand at 4686.900 kb on + strand at 4686.901 kb on - strand at 4687.127 kb on + strand at 4687.127 kb on + strand at 4687.128 kb on - strand at 4687.128 kb on - strand at 4687.171 kb on + strand at 4687.171 kb on + strand at 4687.172 kb on - strand at 4687.176 kb on + strand at 4687.176 kb on + strand at 4687.177 kb on - strand at 4687.312 kb on - strand at 4687.312 kb on - strand at 4687.413 kb on + strand at 4687.413 kb on + strand at 4687.413 kb on + strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.414 kb on - strand at 4687.484 kb on - strand at 4687.484 kb on - strand at 4687.758 kb on + strand, within Pf1N1B4_4230 at 4687.763 kb on - strand, within Pf1N1B4_4230 at 4687.763 kb on - strand, within Pf1N1B4_4230 at 4687.792 kb on - strand, within Pf1N1B4_4230 at 4687.988 kb on + strand, within Pf1N1B4_4230 at 4687.988 kb on + strand, within Pf1N1B4_4230 at 4688.544 kb on + strand at 4688.544 kb on + strand, within Pf1N1B4_4230 at 4688.853 kb on + strand, within Pf1N1B4_4230 at 4689.036 kb on + strand, within Pf1N1B4_4230 at 4689.121 kb on + strand, within Pf1N1B4_4230 at 4689.165 kb on + strand, within Pf1N1B4_4230 at 4689.291 kb on + strand, within Pf1N1B4_4230 at 4689.323 kb on - strand, within Pf1N1B4_4230 at 4689.439 kb on - strand at 4689.439 kb on - strand at 4689.445 kb on - strand at 4689.738 kb on - strand at 4689.739 kb on + strand at 4689.740 kb on - strand at 4689.764 kb on - strand at 4689.789 kb on + strand at 4689.842 kb on + strand at 4689.857 kb on + strand at 4689.858 kb on - strand at 4689.858 kb on - strand at 4689.860 kb on + strand at 4689.860 kb on + strand at 4689.861 kb on - strand at 4689.861 kb on - strand at 4689.870 kb on - strand at 4689.870 kb on - strand at 4689.883 kb on - strand at 4689.959 kb on - strand at 4690.076 kb on + strand at 4690.076 kb on + strand at 4690.076 kb on + strand at 4690.077 kb on - strand at 4690.077 kb on - strand at 4690.077 kb on - strand at 4690.137 kb on + strand at 4690.138 kb on - strand at 4690.138 kb on - strand at 4690.138 kb on - strand at 4690.196 kb on + strand at 4690.196 kb on + strand at 4690.196 kb on + strand at 4690.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 4,686,596 - Pf1N1B4_4229 0.80 -1.6 4,686,702 - Pf1N1B4_4229 0.83 +0.4 4,686,752 - Pf1N1B4_4229 0.85 +0.6 4,686,900 + -1.0 4,686,900 + -0.8 4,686,901 - +1.6 4,687,127 + -0.0 4,687,127 + -0.0 4,687,128 - +2.8 4,687,128 - +0.4 4,687,171 + -0.9 4,687,171 + -1.0 4,687,172 - +0.6 4,687,176 + +1.4 4,687,176 + -0.0 4,687,177 - +1.0 4,687,312 - +0.2 4,687,312 - +0.5 4,687,413 + -1.6 4,687,413 + -0.0 4,687,413 + -1.8 4,687,414 - -0.0 4,687,414 - +0.9 4,687,414 - +0.6 4,687,414 - +0.2 4,687,414 - -1.0 4,687,414 - -0.9 4,687,414 - -2.3 4,687,484 - -0.3 4,687,484 - +0.7 4,687,758 + Pf1N1B4_4230 0.19 +0.6 4,687,763 - Pf1N1B4_4230 0.19 +1.0 4,687,763 - Pf1N1B4_4230 0.19 -1.0 4,687,792 - Pf1N1B4_4230 0.21 +0.5 4,687,988 + Pf1N1B4_4230 0.29 -0.0 4,687,988 + Pf1N1B4_4230 0.29 +0.8 4,688,544 + -1.0 4,688,544 + Pf1N1B4_4230 0.54 -0.8 4,688,853 + Pf1N1B4_4230 0.68 -1.2 4,689,036 + Pf1N1B4_4230 0.76 -0.0 4,689,121 + Pf1N1B4_4230 0.80 +0.4 4,689,165 + Pf1N1B4_4230 0.81 +0.7 4,689,291 + Pf1N1B4_4230 0.87 -0.3 4,689,323 - Pf1N1B4_4230 0.88 +0.3 4,689,439 - +0.4 4,689,439 - -2.0 4,689,445 - -0.8 4,689,738 - -1.0 4,689,739 + +0.4 4,689,740 - -1.6 4,689,764 - -0.3 4,689,789 + -1.2 4,689,842 + -0.3 4,689,857 + +1.6 4,689,858 - +0.2 4,689,858 - -0.0 4,689,860 + -1.0 4,689,860 + -0.5 4,689,861 - -0.0 4,689,861 - +1.6 4,689,870 - +0.7 4,689,870 - -0.0 4,689,883 - +0.7 4,689,959 - +0.2 4,690,076 + -0.0 4,690,076 + -1.2 4,690,076 + -0.8 4,690,077 - -0.0 4,690,077 - -0.0 4,690,077 - -0.0 4,690,137 + -0.3 4,690,138 - +1.0 4,690,138 - -2.0 4,690,138 - -0.7 4,690,196 + -1.6 4,690,196 + +0.2 4,690,196 + -0.0 4,690,357 + +1.4
Or see this region's nucleotide sequence