Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3539 and Pf1N1B4_3540 are separated by 72 nucleotides Pf1N1B4_3540 and Pf1N1B4_3541 are separated by 99 nucleotides Pf1N1B4_3541 and Pf1N1B4_3542 are separated by 191 nucleotides
Pf1N1B4_3539 - Ser/Thr protein phosphatase family protein, UDP-2,3-diacylglucosamine hydrolase (EC 3.6.1.-) homolog, at 3,946,072 to 3,946,887
_3539
Pf1N1B4_3540 - Metal-dependent phosphohydrolase, HD subdomain, at 3,946,960 to 3,947,550
_3540
Pf1N1B4_3541 - FIG027190: Putative transmembrane protein, at 3,947,650 to 3,947,961
_3541
Pf1N1B4_3542 - Transcriptional regulator, AraC family, at 3,948,153 to 3,949,181
_3542
Position (kb)
3947
3948 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3946.747 kb on + strand, within Pf1N1B4_3539 at 3946.747 kb on + strand at 3946.819 kb on + strand at 3946.819 kb on + strand at 3946.866 kb on + strand at 3946.866 kb on + strand at 3946.898 kb on + strand at 3946.898 kb on + strand at 3946.899 kb on - strand at 3946.899 kb on - strand at 3946.899 kb on - strand at 3946.958 kb on + strand at 3946.959 kb on - strand at 3946.959 kb on - strand at 3946.959 kb on - strand at 3946.973 kb on + strand at 3946.974 kb on - strand at 3946.974 kb on - strand at 3947.123 kb on + strand at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.186 kb on + strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.187 kb on - strand, within Pf1N1B4_3540 at 3947.190 kb on - strand, within Pf1N1B4_3540 at 3947.282 kb on + strand, within Pf1N1B4_3540 at 3947.330 kb on + strand, within Pf1N1B4_3540 at 3947.330 kb on + strand at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand, within Pf1N1B4_3540 at 3947.331 kb on - strand at 3947.425 kb on + strand, within Pf1N1B4_3540 at 3947.523 kb on + strand at 3947.524 kb on - strand at 3947.592 kb on + strand at 3947.652 kb on - strand at 3947.652 kb on - strand at 3947.684 kb on + strand, within Pf1N1B4_3541 at 3947.685 kb on - strand, within Pf1N1B4_3541 at 3947.685 kb on - strand, within Pf1N1B4_3541 at 3947.778 kb on + strand at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.778 kb on + strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.779 kb on - strand, within Pf1N1B4_3541 at 3947.913 kb on - strand, within Pf1N1B4_3541 at 3948.015 kb on + strand at 3948.015 kb on + strand at 3948.016 kb on - strand at 3948.019 kb on + strand at 3948.020 kb on - strand at 3948.020 kb on - strand at 3948.057 kb on - strand at 3948.068 kb on + strand at 3948.069 kb on - strand at 3948.109 kb on + strand at 3948.113 kb on - strand at 3948.225 kb on + strand at 3948.483 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.648 kb on + strand, within Pf1N1B4_3542 at 3948.649 kb on - strand, within Pf1N1B4_3542 at 3948.690 kb on + strand, within Pf1N1B4_3542 at 3948.691 kb on - strand at 3948.691 kb on - strand, within Pf1N1B4_3542 at 3948.786 kb on + strand, within Pf1N1B4_3542 at 3948.787 kb on - strand, within Pf1N1B4_3542 at 3948.838 kb on + strand at 3948.838 kb on + strand, within Pf1N1B4_3542 at 3948.875 kb on + strand, within Pf1N1B4_3542
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 3,946,747 + Pf1N1B4_3539 0.83 +0.5 3,946,747 + -0.8 3,946,819 + -0.8 3,946,819 + +0.6 3,946,866 + -1.1 3,946,866 + +2.0 3,946,898 + +0.2 3,946,898 + -0.7 3,946,899 - +1.5 3,946,899 - +0.2 3,946,899 - -0.8 3,946,958 + -0.2 3,946,959 - -2.1 3,946,959 - +1.2 3,946,959 - +0.2 3,946,973 + -1.8 3,946,974 - +0.5 3,946,974 - -1.8 3,947,123 + +0.2 3,947,186 + Pf1N1B4_3540 0.38 +0.8 3,947,186 + Pf1N1B4_3540 0.38 -0.5 3,947,186 + Pf1N1B4_3540 0.38 +1.2 3,947,187 - Pf1N1B4_3540 0.38 -0.2 3,947,187 - Pf1N1B4_3540 0.38 +0.5 3,947,187 - Pf1N1B4_3540 0.38 +1.2 3,947,187 - Pf1N1B4_3540 0.38 +0.2 3,947,190 - Pf1N1B4_3540 0.39 +2.0 3,947,282 + Pf1N1B4_3540 0.54 +0.2 3,947,330 + Pf1N1B4_3540 0.63 -0.8 3,947,330 + +0.2 3,947,331 - Pf1N1B4_3540 0.63 -0.8 3,947,331 - Pf1N1B4_3540 0.63 -1.0 3,947,331 - Pf1N1B4_3540 0.63 -1.1 3,947,331 - Pf1N1B4_3540 0.63 +0.2 3,947,331 - Pf1N1B4_3540 0.63 -1.4 3,947,331 - Pf1N1B4_3540 0.63 -1.1 3,947,331 - -0.8 3,947,425 + Pf1N1B4_3540 0.79 +0.2 3,947,523 + -0.5 3,947,524 - +0.2 3,947,592 + -0.4 3,947,652 - +0.8 3,947,652 - -1.4 3,947,684 + Pf1N1B4_3541 0.11 +0.8 3,947,685 - Pf1N1B4_3541 0.11 +1.4 3,947,685 - Pf1N1B4_3541 0.11 -0.8 3,947,778 + +1.8 3,947,778 + Pf1N1B4_3541 0.41 +0.2 3,947,778 + Pf1N1B4_3541 0.41 +2.0 3,947,778 + Pf1N1B4_3541 0.41 -1.8 3,947,779 - Pf1N1B4_3541 0.41 -1.4 3,947,779 - Pf1N1B4_3541 0.41 +1.1 3,947,779 - Pf1N1B4_3541 0.41 -1.4 3,947,779 - Pf1N1B4_3541 0.41 +1.2 3,947,913 - Pf1N1B4_3541 0.84 -0.2 3,948,015 + +0.2 3,948,015 + -1.4 3,948,016 - -1.4 3,948,019 + -0.8 3,948,020 - +2.2 3,948,020 - -1.4 3,948,057 - +0.8 3,948,068 + +1.2 3,948,069 - -0.2 3,948,109 + +0.2 3,948,113 - -1.4 3,948,225 + +0.6 3,948,483 + Pf1N1B4_3542 0.32 -0.5 3,948,648 + Pf1N1B4_3542 0.48 -0.8 3,948,648 + Pf1N1B4_3542 0.48 +0.2 3,948,648 + Pf1N1B4_3542 0.48 +0.8 3,948,648 + +1.2 3,948,648 + Pf1N1B4_3542 0.48 +0.9 3,948,648 + Pf1N1B4_3542 0.48 +1.1 3,948,648 + Pf1N1B4_3542 0.48 -0.2 3,948,648 + Pf1N1B4_3542 0.48 +0.2 3,948,649 - Pf1N1B4_3542 0.48 -0.6 3,948,690 + Pf1N1B4_3542 0.52 -1.1 3,948,691 - +0.2 3,948,691 - Pf1N1B4_3542 0.52 +0.8 3,948,786 + Pf1N1B4_3542 0.62 -1.7 3,948,787 - Pf1N1B4_3542 0.62 +0.6 3,948,838 + -0.8 3,948,838 + Pf1N1B4_3542 0.67 +0.4 3,948,875 + Pf1N1B4_3542 0.70 -0.5
Or see this region's nucleotide sequence