Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2726 and Pf1N1B4_2727 are separated by 30 nucleotides Pf1N1B4_2727 and Pf1N1B4_2728 are separated by 97 nucleotides Pf1N1B4_2728 and Pf1N1B4_2729 are separated by 141 nucleotides
Pf1N1B4_2726 - Cobalt-zinc-cadmium resistance protein, at 3,064,406 to 3,065,299
_2726
Pf1N1B4_2727 - Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase), at 3,065,330 to 3,065,749
_2727
Pf1N1B4_2728 - ATP-dependent helicase HrpB, at 3,065,847 to 3,068,363
_2728
Pf1N1B4_2729 - endonuclease/exonuclease/phosphatase family protein, at 3,068,505 to 3,069,587
_2729
Position (kb)
3065
3066
3067
3068
3069 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3065.110 kb on - strand, within Pf1N1B4_2726 at 3065.110 kb on - strand, within Pf1N1B4_2726 at 3065.267 kb on + strand at 3065.299 kb on + strand at 3065.299 kb on + strand at 3065.299 kb on + strand at 3065.300 kb on - strand at 3065.300 kb on - strand at 3065.300 kb on - strand at 3065.300 kb on - strand at 3065.440 kb on + strand, within Pf1N1B4_2727 at 3065.440 kb on + strand, within Pf1N1B4_2727 at 3065.441 kb on - strand, within Pf1N1B4_2727 at 3065.441 kb on - strand, within Pf1N1B4_2727 at 3065.441 kb on - strand, within Pf1N1B4_2727 at 3065.533 kb on - strand, within Pf1N1B4_2727 at 3065.533 kb on - strand, within Pf1N1B4_2727 at 3065.533 kb on - strand, within Pf1N1B4_2727 at 3065.535 kb on + strand, within Pf1N1B4_2727 at 3065.535 kb on + strand, within Pf1N1B4_2727 at 3065.535 kb on + strand, within Pf1N1B4_2727 at 3065.536 kb on - strand at 3065.536 kb on - strand, within Pf1N1B4_2727 at 3065.588 kb on - strand, within Pf1N1B4_2727 at 3065.589 kb on + strand, within Pf1N1B4_2727 at 3065.589 kb on + strand, within Pf1N1B4_2727 at 3065.589 kb on + strand, within Pf1N1B4_2727 at 3065.590 kb on - strand, within Pf1N1B4_2727 at 3065.590 kb on - strand, within Pf1N1B4_2727 at 3065.717 kb on + strand at 3065.717 kb on + strand at 3065.717 kb on + strand at 3065.797 kb on + strand at 3065.797 kb on + strand at 3065.797 kb on + strand at 3065.797 kb on + strand at 3065.798 kb on - strand at 3065.798 kb on - strand at 3065.798 kb on - strand at 3065.819 kb on - strand at 3065.820 kb on + strand at 3065.820 kb on + strand at 3065.820 kb on + strand at 3065.821 kb on - strand at 3065.821 kb on - strand at 3065.821 kb on - strand at 3065.821 kb on - strand at 3065.821 kb on - strand at 3065.900 kb on + strand at 3066.314 kb on - strand, within Pf1N1B4_2728 at 3066.314 kb on - strand, within Pf1N1B4_2728 at 3066.314 kb on - strand, within Pf1N1B4_2728 at 3066.314 kb on - strand, within Pf1N1B4_2728 at 3066.396 kb on + strand, within Pf1N1B4_2728 at 3066.397 kb on - strand, within Pf1N1B4_2728 at 3066.443 kb on + strand, within Pf1N1B4_2728 at 3066.903 kb on + strand, within Pf1N1B4_2728 at 3067.017 kb on + strand, within Pf1N1B4_2728 at 3067.017 kb on + strand, within Pf1N1B4_2728 at 3067.017 kb on + strand, within Pf1N1B4_2728 at 3067.017 kb on + strand, within Pf1N1B4_2728 at 3067.017 kb on + strand, within Pf1N1B4_2728 at 3067.017 kb on + strand, within Pf1N1B4_2728 at 3067.018 kb on - strand, within Pf1N1B4_2728 at 3067.018 kb on - strand, within Pf1N1B4_2728 at 3067.018 kb on - strand, within Pf1N1B4_2728 at 3067.203 kb on - strand, within Pf1N1B4_2728 at 3067.452 kb on + strand, within Pf1N1B4_2728 at 3067.569 kb on + strand, within Pf1N1B4_2728 at 3067.570 kb on - strand, within Pf1N1B4_2728 at 3067.865 kb on + strand, within Pf1N1B4_2728 at 3067.961 kb on + strand, within Pf1N1B4_2728 at 3067.961 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.091 kb on + strand, within Pf1N1B4_2728 at 3068.259 kb on + strand at 3068.259 kb on + strand at 3068.260 kb on - strand at 3068.260 kb on - strand at 3068.304 kb on + strand at 3068.361 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.568 kb on - strand at 3068.568 kb on - strand at 3068.615 kb on - strand, within Pf1N1B4_2729 at 3068.624 kb on - strand, within Pf1N1B4_2729 at 3068.624 kb on - strand, within Pf1N1B4_2729 at 3068.624 kb on - strand, within Pf1N1B4_2729 at 3068.732 kb on - strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.770 kb on + strand, within Pf1N1B4_2729 at 3068.771 kb on - strand, within Pf1N1B4_2729 at 3068.803 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3068.867 kb on - strand at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3069.054 kb on + strand, within Pf1N1B4_2729 at 3069.124 kb on + strand, within Pf1N1B4_2729 at 3069.124 kb on + strand, within Pf1N1B4_2729 at 3069.229 kb on + strand, within Pf1N1B4_2729
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 3,065,110 - Pf1N1B4_2726 0.79 -0.4 3,065,110 - Pf1N1B4_2726 0.79 +1.4 3,065,267 + -1.5 3,065,299 + -1.7 3,065,299 + +1.2 3,065,299 + +0.6 3,065,300 - +0.1 3,065,300 - -1.0 3,065,300 - +0.4 3,065,300 - -1.0 3,065,440 + Pf1N1B4_2727 0.26 +0.0 3,065,440 + Pf1N1B4_2727 0.26 +0.6 3,065,441 - Pf1N1B4_2727 0.26 +0.0 3,065,441 - Pf1N1B4_2727 0.26 -1.4 3,065,441 - Pf1N1B4_2727 0.26 -0.4 3,065,533 - Pf1N1B4_2727 0.48 +0.3 3,065,533 - Pf1N1B4_2727 0.48 +0.2 3,065,533 - Pf1N1B4_2727 0.48 -0.1 3,065,535 + Pf1N1B4_2727 0.49 -1.7 3,065,535 + Pf1N1B4_2727 0.49 -0.1 3,065,535 + Pf1N1B4_2727 0.49 +2.2 3,065,536 - +0.6 3,065,536 - Pf1N1B4_2727 0.49 -1.7 3,065,588 - Pf1N1B4_2727 0.61 -1.8 3,065,589 + Pf1N1B4_2727 0.62 -0.4 3,065,589 + Pf1N1B4_2727 0.62 +1.8 3,065,589 + Pf1N1B4_2727 0.62 +1.2 3,065,590 - Pf1N1B4_2727 0.62 -0.4 3,065,590 - Pf1N1B4_2727 0.62 +0.6 3,065,717 + -0.4 3,065,717 + -0.7 3,065,717 + -1.0 3,065,797 + -0.6 3,065,797 + -0.4 3,065,797 + -1.6 3,065,797 + -0.6 3,065,798 - -1.1 3,065,798 - -0.0 3,065,798 - +0.4 3,065,819 - -0.5 3,065,820 + +0.6 3,065,820 + +0.6 3,065,820 + +0.6 3,065,821 - -1.0 3,065,821 - -1.4 3,065,821 - -0.4 3,065,821 - +0.0 3,065,821 - -1.0 3,065,900 + -2.0 3,066,314 - Pf1N1B4_2728 0.19 +2.2 3,066,314 - Pf1N1B4_2728 0.19 +1.4 3,066,314 - Pf1N1B4_2728 0.19 +0.6 3,066,314 - Pf1N1B4_2728 0.19 +2.2 3,066,396 + Pf1N1B4_2728 0.22 +0.4 3,066,397 - Pf1N1B4_2728 0.22 +3.2 3,066,443 + Pf1N1B4_2728 0.24 +0.6 3,066,903 + Pf1N1B4_2728 0.42 +1.6 3,067,017 + Pf1N1B4_2728 0.46 -1.4 3,067,017 + Pf1N1B4_2728 0.46 -1.0 3,067,017 + Pf1N1B4_2728 0.46 -0.4 3,067,017 + Pf1N1B4_2728 0.46 -1.0 3,067,017 + Pf1N1B4_2728 0.46 +0.6 3,067,017 + Pf1N1B4_2728 0.46 -1.7 3,067,018 - Pf1N1B4_2728 0.47 +0.4 3,067,018 - Pf1N1B4_2728 0.47 +1.0 3,067,018 - Pf1N1B4_2728 0.47 +0.3 3,067,203 - Pf1N1B4_2728 0.54 +0.6 3,067,452 + Pf1N1B4_2728 0.64 -2.0 3,067,569 + Pf1N1B4_2728 0.68 -1.0 3,067,570 - Pf1N1B4_2728 0.68 -0.4 3,067,865 + Pf1N1B4_2728 0.80 -2.4 3,067,961 + Pf1N1B4_2728 0.84 +0.1 3,067,961 + Pf1N1B4_2728 0.84 -0.4 3,068,000 + Pf1N1B4_2728 0.86 -0.7 3,068,000 + Pf1N1B4_2728 0.86 +0.9 3,068,000 + Pf1N1B4_2728 0.86 +0.6 3,068,000 + Pf1N1B4_2728 0.86 +0.6 3,068,000 + Pf1N1B4_2728 0.86 -1.0 3,068,000 + Pf1N1B4_2728 0.86 -0.2 3,068,091 + Pf1N1B4_2728 0.89 +1.2 3,068,259 + -1.4 3,068,259 + -1.4 3,068,260 - +1.6 3,068,260 - -0.4 3,068,304 + -0.3 3,068,361 + +0.6 3,068,433 + -1.4 3,068,433 + -0.7 3,068,433 + -0.2 3,068,433 + +0.0 3,068,433 + +2.2 3,068,433 + +0.6 3,068,433 + -1.0 3,068,433 + -0.7 3,068,433 + -0.4 3,068,433 + +0.4 3,068,433 + +0.6 3,068,433 + -0.4 3,068,433 + -1.0 3,068,433 + -0.4 3,068,433 + -0.6 3,068,433 + +1.0 3,068,433 + -2.2 3,068,433 + +1.2 3,068,433 + -0.4 3,068,433 + -0.4 3,068,433 + +0.6 3,068,433 + -1.4 3,068,434 - -1.4 3,068,434 - +0.0 3,068,434 - +2.2 3,068,434 - -0.1 3,068,434 - +0.0 3,068,434 - +0.0 3,068,434 - +0.5 3,068,434 - -1.4 3,068,434 - +0.0 3,068,434 - +0.6 3,068,434 - -0.1 3,068,434 - +0.5 3,068,434 - -1.7 3,068,434 - -1.0 3,068,434 - -0.4 3,068,434 - -0.1 3,068,434 - +0.6 3,068,434 - +0.3 3,068,434 - -1.7 3,068,434 - -0.4 3,068,434 - +0.6 3,068,434 - +0.6 3,068,434 - +1.2 3,068,434 - -0.1 3,068,567 + +1.6 3,068,567 + +1.6 3,068,567 + +0.9 3,068,567 + -1.7 3,068,567 + +0.0 3,068,567 + -0.6 3,068,567 + +1.2 3,068,567 + +0.6 3,068,567 + -0.4 3,068,567 + +0.6 3,068,567 + +0.0 3,068,568 - -0.8 3,068,568 - -2.0 3,068,615 - Pf1N1B4_2729 0.10 -0.7 3,068,624 - Pf1N1B4_2729 0.11 -2.2 3,068,624 - Pf1N1B4_2729 0.11 +1.4 3,068,624 - Pf1N1B4_2729 0.11 -1.7 3,068,732 - Pf1N1B4_2729 0.21 +1.6 3,068,747 + Pf1N1B4_2729 0.22 -1.4 3,068,747 + Pf1N1B4_2729 0.22 -1.0 3,068,747 + Pf1N1B4_2729 0.22 +0.6 3,068,747 + Pf1N1B4_2729 0.22 +0.9 3,068,747 + Pf1N1B4_2729 0.22 -0.2 3,068,747 + Pf1N1B4_2729 0.22 -1.0 3,068,748 - Pf1N1B4_2729 0.22 +0.6 3,068,748 - Pf1N1B4_2729 0.22 +1.3 3,068,748 - Pf1N1B4_2729 0.22 +0.6 3,068,748 - Pf1N1B4_2729 0.22 +0.6 3,068,748 - Pf1N1B4_2729 0.22 -0.1 3,068,748 - Pf1N1B4_2729 0.22 -1.4 3,068,748 - -0.4 3,068,748 - Pf1N1B4_2729 0.22 -0.6 3,068,748 - Pf1N1B4_2729 0.22 +0.4 3,068,748 - Pf1N1B4_2729 0.22 -1.6 3,068,770 + Pf1N1B4_2729 0.24 +3.2 3,068,771 - Pf1N1B4_2729 0.25 -1.7 3,068,803 + Pf1N1B4_2729 0.28 +0.6 3,068,866 + Pf1N1B4_2729 0.33 -1.7 3,068,866 + Pf1N1B4_2729 0.33 +0.1 3,068,866 + Pf1N1B4_2729 0.33 -1.4 3,068,866 + Pf1N1B4_2729 0.33 +0.0 3,068,866 + Pf1N1B4_2729 0.33 +0.6 3,068,866 + Pf1N1B4_2729 0.33 +0.6 3,068,867 - Pf1N1B4_2729 0.33 -2.4 3,068,867 - +0.6 3,068,867 - Pf1N1B4_2729 0.33 +1.6 3,068,867 - Pf1N1B4_2729 0.33 -0.1 3,068,867 - Pf1N1B4_2729 0.33 -2.4 3,069,054 + Pf1N1B4_2729 0.51 +0.4 3,069,124 + Pf1N1B4_2729 0.57 -1.5 3,069,124 + Pf1N1B4_2729 0.57 -0.2 3,069,229 + Pf1N1B4_2729 0.67 +1.6
Or see this region's nucleotide sequence