Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2493 and Pf1N1B4_2494 are separated by 228 nucleotides Pf1N1B4_2494 and Pf1N1B4_2495 are separated by 84 nucleotides
Pf1N1B4_2493 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 2,825,260 to 2,827,065
_2493
Pf1N1B4_2494 - GGDEF domain protein, at 2,827,294 to 2,828,583
_2494
Pf1N1B4_2495 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 2,828,668 to 2,830,470
_2495
Position (kb)
2827
2828
2829 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2826.296 kb on + strand, within Pf1N1B4_2493 at 2826.497 kb on + strand, within Pf1N1B4_2493 at 2826.577 kb on + strand, within Pf1N1B4_2493 at 2826.621 kb on + strand, within Pf1N1B4_2493 at 2826.835 kb on + strand, within Pf1N1B4_2493 at 2826.859 kb on + strand, within Pf1N1B4_2493 at 2826.959 kb on - strand at 2826.970 kb on - strand at 2826.998 kb on + strand at 2826.998 kb on + strand at 2827.060 kb on + strand at 2827.060 kb on + strand at 2827.060 kb on + strand at 2827.061 kb on - strand at 2827.064 kb on - strand at 2827.171 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.217 kb on - strand at 2827.237 kb on - strand at 2827.237 kb on - strand at 2827.237 kb on - strand at 2827.313 kb on + strand at 2827.313 kb on + strand at 2827.340 kb on + strand at 2827.341 kb on - strand at 2827.347 kb on - strand at 2827.347 kb on - strand at 2827.347 kb on - strand at 2827.347 kb on - strand at 2827.372 kb on + strand at 2827.372 kb on + strand at 2827.372 kb on + strand at 2827.373 kb on - strand at 2827.373 kb on - strand at 2827.373 kb on - strand at 2827.422 kb on + strand at 2827.422 kb on + strand at 2827.422 kb on + strand at 2827.422 kb on + strand at 2827.422 kb on + strand at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.423 kb on - strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.501 kb on + strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.502 kb on - strand, within Pf1N1B4_2494 at 2827.511 kb on - strand, within Pf1N1B4_2494 at 2827.537 kb on + strand, within Pf1N1B4_2494 at 2827.538 kb on - strand, within Pf1N1B4_2494 at 2827.538 kb on - strand, within Pf1N1B4_2494 at 2827.538 kb on - strand, within Pf1N1B4_2494 at 2827.541 kb on - strand, within Pf1N1B4_2494 at 2827.541 kb on - strand at 2827.610 kb on + strand, within Pf1N1B4_2494 at 2827.610 kb on + strand, within Pf1N1B4_2494 at 2827.611 kb on - strand, within Pf1N1B4_2494 at 2827.611 kb on - strand, within Pf1N1B4_2494 at 2828.008 kb on + strand, within Pf1N1B4_2494 at 2828.009 kb on - strand, within Pf1N1B4_2494 at 2828.092 kb on + strand, within Pf1N1B4_2494 at 2828.092 kb on + strand, within Pf1N1B4_2494 at 2828.222 kb on - strand, within Pf1N1B4_2494 at 2828.280 kb on + strand, within Pf1N1B4_2494 at 2828.280 kb on + strand, within Pf1N1B4_2494 at 2828.280 kb on + strand, within Pf1N1B4_2494 at 2828.280 kb on + strand, within Pf1N1B4_2494 at 2828.280 kb on + strand, within Pf1N1B4_2494 at 2828.280 kb on + strand, within Pf1N1B4_2494 at 2828.281 kb on - strand, within Pf1N1B4_2494 at 2828.281 kb on - strand, within Pf1N1B4_2494 at 2828.281 kb on - strand, within Pf1N1B4_2494 at 2828.281 kb on - strand, within Pf1N1B4_2494 at 2828.281 kb on - strand, within Pf1N1B4_2494 at 2828.320 kb on + strand, within Pf1N1B4_2494 at 2828.321 kb on - strand, within Pf1N1B4_2494 at 2828.321 kb on - strand, within Pf1N1B4_2494 at 2828.321 kb on - strand, within Pf1N1B4_2494 at 2828.321 kb on - strand, within Pf1N1B4_2494 at 2828.321 kb on - strand, within Pf1N1B4_2494 at 2828.321 kb on - strand, within Pf1N1B4_2494 at 2828.408 kb on + strand, within Pf1N1B4_2494 at 2828.494 kb on + strand at 2828.494 kb on + strand at 2828.494 kb on + strand at 2828.495 kb on - strand at 2828.640 kb on + strand at 2828.640 kb on + strand at 2828.640 kb on + strand at 2828.704 kb on - strand at 2828.746 kb on + strand at 2828.746 kb on + strand at 2828.747 kb on - strand at 2828.747 kb on - strand at 2828.747 kb on - strand at 2828.898 kb on + strand, within Pf1N1B4_2495 at 2828.926 kb on + strand at 2828.926 kb on + strand, within Pf1N1B4_2495 at 2828.926 kb on + strand, within Pf1N1B4_2495 at 2828.927 kb on - strand, within Pf1N1B4_2495 at 2829.033 kb on + strand at 2829.033 kb on + strand, within Pf1N1B4_2495 at 2829.033 kb on + strand at 2829.034 kb on - strand, within Pf1N1B4_2495 at 2829.034 kb on - strand, within Pf1N1B4_2495 at 2829.050 kb on - strand, within Pf1N1B4_2495 at 2829.118 kb on + strand, within Pf1N1B4_2495 at 2829.119 kb on - strand, within Pf1N1B4_2495 at 2829.119 kb on - strand at 2829.119 kb on - strand, within Pf1N1B4_2495 at 2829.119 kb on - strand, within Pf1N1B4_2495 at 2829.119 kb on - strand at 2829.369 kb on + strand, within Pf1N1B4_2495 at 2829.369 kb on + strand, within Pf1N1B4_2495 at 2829.369 kb on + strand, within Pf1N1B4_2495 at 2829.370 kb on - strand, within Pf1N1B4_2495 at 2829.373 kb on + strand, within Pf1N1B4_2495 at 2829.373 kb on + strand, within Pf1N1B4_2495 at 2829.373 kb on + strand, within Pf1N1B4_2495 at 2829.374 kb on - strand, within Pf1N1B4_2495 at 2829.374 kb on - strand, within Pf1N1B4_2495 at 2829.374 kb on - strand, within Pf1N1B4_2495 at 2829.374 kb on - strand, within Pf1N1B4_2495 at 2829.374 kb on - strand, within Pf1N1B4_2495
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 2,826,296 + Pf1N1B4_2493 0.57 +0.6 2,826,497 + Pf1N1B4_2493 0.68 +0.6 2,826,577 + Pf1N1B4_2493 0.73 -2.0 2,826,621 + Pf1N1B4_2493 0.75 -3.1 2,826,835 + Pf1N1B4_2493 0.87 +0.6 2,826,859 + Pf1N1B4_2493 0.89 -1.4 2,826,959 - -0.4 2,826,970 - +0.6 2,826,998 + -0.4 2,826,998 + -1.4 2,827,060 + +0.9 2,827,060 + +1.6 2,827,060 + +0.6 2,827,061 - -1.0 2,827,064 - +0.6 2,827,171 - +1.6 2,827,217 - -0.3 2,827,217 - -0.4 2,827,217 - -1.0 2,827,217 - +0.6 2,827,217 - +0.2 2,827,217 - +0.3 2,827,217 - +0.0 2,827,237 - +0.6 2,827,237 - -1.7 2,827,237 - +0.6 2,827,313 + -0.4 2,827,313 + +0.0 2,827,340 + +0.6 2,827,341 - -0.4 2,827,347 - -0.4 2,827,347 - +0.6 2,827,347 - -0.8 2,827,347 - -0.1 2,827,372 + -1.7 2,827,372 + +0.9 2,827,372 + +0.6 2,827,373 - -2.6 2,827,373 - -0.4 2,827,373 - -1.0 2,827,422 + +0.6 2,827,422 + +0.6 2,827,422 + -0.1 2,827,422 + -0.7 2,827,422 + -0.4 2,827,423 - Pf1N1B4_2494 0.10 +0.6 2,827,423 - Pf1N1B4_2494 0.10 -1.4 2,827,423 - Pf1N1B4_2494 0.10 -0.9 2,827,423 - Pf1N1B4_2494 0.10 +0.6 2,827,423 - Pf1N1B4_2494 0.10 +0.6 2,827,423 - Pf1N1B4_2494 0.10 +1.6 2,827,501 + Pf1N1B4_2494 0.16 -0.4 2,827,501 + Pf1N1B4_2494 0.16 +1.4 2,827,501 + Pf1N1B4_2494 0.16 +0.6 2,827,501 + Pf1N1B4_2494 0.16 +0.4 2,827,501 + Pf1N1B4_2494 0.16 +0.6 2,827,501 + Pf1N1B4_2494 0.16 -1.0 2,827,501 + Pf1N1B4_2494 0.16 -1.2 2,827,501 + Pf1N1B4_2494 0.16 +1.3 2,827,501 + Pf1N1B4_2494 0.16 +1.2 2,827,502 - Pf1N1B4_2494 0.16 +0.6 2,827,502 - Pf1N1B4_2494 0.16 -0.4 2,827,502 - Pf1N1B4_2494 0.16 +1.6 2,827,502 - Pf1N1B4_2494 0.16 +2.2 2,827,502 - Pf1N1B4_2494 0.16 +0.6 2,827,502 - Pf1N1B4_2494 0.16 +0.0 2,827,502 - Pf1N1B4_2494 0.16 -0.4 2,827,511 - Pf1N1B4_2494 0.17 +2.2 2,827,537 + Pf1N1B4_2494 0.19 +0.6 2,827,538 - Pf1N1B4_2494 0.19 -0.4 2,827,538 - Pf1N1B4_2494 0.19 -0.1 2,827,538 - Pf1N1B4_2494 0.19 +0.9 2,827,541 - Pf1N1B4_2494 0.19 -1.7 2,827,541 - +0.6 2,827,610 + Pf1N1B4_2494 0.24 +1.3 2,827,610 + Pf1N1B4_2494 0.24 -1.4 2,827,611 - Pf1N1B4_2494 0.25 +0.6 2,827,611 - Pf1N1B4_2494 0.25 +0.0 2,828,008 + Pf1N1B4_2494 0.55 -0.7 2,828,009 - Pf1N1B4_2494 0.55 -1.4 2,828,092 + Pf1N1B4_2494 0.62 -0.1 2,828,092 + Pf1N1B4_2494 0.62 +0.4 2,828,222 - Pf1N1B4_2494 0.72 -0.2 2,828,280 + Pf1N1B4_2494 0.76 -0.4 2,828,280 + Pf1N1B4_2494 0.76 -0.7 2,828,280 + Pf1N1B4_2494 0.76 +0.6 2,828,280 + Pf1N1B4_2494 0.76 +1.6 2,828,280 + Pf1N1B4_2494 0.76 -2.2 2,828,280 + Pf1N1B4_2494 0.76 +0.6 2,828,281 - Pf1N1B4_2494 0.77 -0.4 2,828,281 - Pf1N1B4_2494 0.77 -1.7 2,828,281 - Pf1N1B4_2494 0.77 -1.4 2,828,281 - Pf1N1B4_2494 0.77 +0.0 2,828,281 - Pf1N1B4_2494 0.77 +1.2 2,828,320 + Pf1N1B4_2494 0.80 +0.5 2,828,321 - Pf1N1B4_2494 0.80 +1.6 2,828,321 - Pf1N1B4_2494 0.80 -0.7 2,828,321 - Pf1N1B4_2494 0.80 -1.6 2,828,321 - Pf1N1B4_2494 0.80 +0.6 2,828,321 - Pf1N1B4_2494 0.80 +0.0 2,828,321 - Pf1N1B4_2494 0.80 +3.2 2,828,408 + Pf1N1B4_2494 0.86 +0.1 2,828,494 + -1.4 2,828,494 + -2.4 2,828,494 + -1.4 2,828,495 - +0.6 2,828,640 + +0.0 2,828,640 + +1.0 2,828,640 + -1.7 2,828,704 - +1.2 2,828,746 + +2.6 2,828,746 + -1.4 2,828,747 - -0.4 2,828,747 - -0.6 2,828,747 - +0.6 2,828,898 + Pf1N1B4_2495 0.13 -0.7 2,828,926 + +0.6 2,828,926 + Pf1N1B4_2495 0.14 +2.2 2,828,926 + Pf1N1B4_2495 0.14 +0.6 2,828,927 - Pf1N1B4_2495 0.14 -1.2 2,829,033 + +0.6 2,829,033 + Pf1N1B4_2495 0.20 -1.6 2,829,033 + +0.6 2,829,034 - Pf1N1B4_2495 0.20 -1.1 2,829,034 - Pf1N1B4_2495 0.20 +0.0 2,829,050 - Pf1N1B4_2495 0.21 -0.4 2,829,118 + Pf1N1B4_2495 0.25 -0.4 2,829,119 - Pf1N1B4_2495 0.25 +1.9 2,829,119 - -1.0 2,829,119 - Pf1N1B4_2495 0.25 +1.5 2,829,119 - Pf1N1B4_2495 0.25 +0.4 2,829,119 - +0.6 2,829,369 + Pf1N1B4_2495 0.39 -2.2 2,829,369 + Pf1N1B4_2495 0.39 -0.5 2,829,369 + Pf1N1B4_2495 0.39 +1.0 2,829,370 - Pf1N1B4_2495 0.39 -1.3 2,829,373 + Pf1N1B4_2495 0.39 -1.7 2,829,373 + Pf1N1B4_2495 0.39 +1.1 2,829,373 + Pf1N1B4_2495 0.39 -1.1 2,829,374 - Pf1N1B4_2495 0.39 +2.2 2,829,374 - Pf1N1B4_2495 0.39 -0.4 2,829,374 - Pf1N1B4_2495 0.39 -0.1 2,829,374 - Pf1N1B4_2495 0.39 +0.5 2,829,374 - Pf1N1B4_2495 0.39 -1.0
Or see this region's nucleotide sequence