Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_849

Experiment: L-Leucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_847 and Pf1N1B4_848 are separated by 164 nucleotidesPf1N1B4_848 and Pf1N1B4_849 overlap by 13 nucleotidesPf1N1B4_849 and Pf1N1B4_850 are separated by 151 nucleotides Pf1N1B4_847 - 2-isopropylmalate synthase (EC 2.3.3.13), at 974,985 to 976,664 _847 Pf1N1B4_848 - Aliphatic amidase AmiE (EC 3.5.1.4), at 976,829 to 977,620 _848 Pf1N1B4_849 - Aspartate aminotransferase (EC 2.6.1.1), at 977,608 to 978,756 _849 Pf1N1B4_850 - GTP-binding protein EngA, at 978,908 to 980,377 _850 Position (kb) 977 978 979Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 976.988 kb on - strand, within Pf1N1B4_848at 976.989 kb on + strand, within Pf1N1B4_848at 976.990 kb on - strand, within Pf1N1B4_848at 976.990 kb on - strand, within Pf1N1B4_848at 976.990 kb on - strand, within Pf1N1B4_848at 976.990 kb on - strand, within Pf1N1B4_848at 977.037 kb on + strand, within Pf1N1B4_848at 977.037 kb on + strandat 977.038 kb on - strand, within Pf1N1B4_848at 977.038 kb on - strand, within Pf1N1B4_848at 977.038 kb on - strand, within Pf1N1B4_848at 977.038 kb on - strand, within Pf1N1B4_848at 977.038 kb on - strand, within Pf1N1B4_848at 977.116 kb on - strand, within Pf1N1B4_848at 977.173 kb on - strand, within Pf1N1B4_848at 977.283 kb on + strand, within Pf1N1B4_848at 977.284 kb on - strand, within Pf1N1B4_848at 977.410 kb on + strand, within Pf1N1B4_848at 977.410 kb on + strand, within Pf1N1B4_848at 977.411 kb on - strand, within Pf1N1B4_848at 977.620 kb on + strandat 977.621 kb on - strandat 977.621 kb on - strandat 977.621 kb on - strandat 977.705 kb on + strandat 977.705 kb on + strandat 977.705 kb on + strandat 977.705 kb on + strandat 977.816 kb on + strand, within Pf1N1B4_849at 977.816 kb on + strand, within Pf1N1B4_849at 977.816 kb on + strandat 977.816 kb on + strand, within Pf1N1B4_849at 977.816 kb on + strand, within Pf1N1B4_849at 977.816 kb on + strand, within Pf1N1B4_849at 977.816 kb on + strand, within Pf1N1B4_849at 977.816 kb on + strand, within Pf1N1B4_849at 977.816 kb on + strand, within Pf1N1B4_849at 977.816 kb on + strand, within Pf1N1B4_849at 977.817 kb on - strand, within Pf1N1B4_849at 977.817 kb on - strand, within Pf1N1B4_849at 977.817 kb on - strand, within Pf1N1B4_849at 977.821 kb on - strand, within Pf1N1B4_849at 977.891 kb on + strand, within Pf1N1B4_849at 977.891 kb on + strand, within Pf1N1B4_849at 977.891 kb on + strand, within Pf1N1B4_849at 977.892 kb on - strand, within Pf1N1B4_849at 977.892 kb on - strand, within Pf1N1B4_849at 977.892 kb on - strand, within Pf1N1B4_849at 977.936 kb on + strand, within Pf1N1B4_849at 977.937 kb on - strand, within Pf1N1B4_849at 977.937 kb on - strand, within Pf1N1B4_849at 977.937 kb on - strand, within Pf1N1B4_849at 977.937 kb on - strand, within Pf1N1B4_849at 977.951 kb on + strand, within Pf1N1B4_849at 977.952 kb on - strand, within Pf1N1B4_849at 977.952 kb on - strand, within Pf1N1B4_849at 977.984 kb on + strand, within Pf1N1B4_849at 977.984 kb on + strand, within Pf1N1B4_849at 977.985 kb on - strand, within Pf1N1B4_849at 977.985 kb on - strand, within Pf1N1B4_849at 978.036 kb on - strand, within Pf1N1B4_849at 978.062 kb on + strand, within Pf1N1B4_849at 978.062 kb on + strand, within Pf1N1B4_849at 978.063 kb on - strand, within Pf1N1B4_849at 978.063 kb on - strand, within Pf1N1B4_849at 978.122 kb on - strand, within Pf1N1B4_849at 978.137 kb on - strand, within Pf1N1B4_849at 978.137 kb on - strand, within Pf1N1B4_849at 978.137 kb on - strand, within Pf1N1B4_849at 978.148 kb on + strand, within Pf1N1B4_849at 978.162 kb on - strandat 978.182 kb on + strandat 978.182 kb on + strandat 978.182 kb on + strand, within Pf1N1B4_849at 978.182 kb on + strand, within Pf1N1B4_849at 978.182 kb on + strand, within Pf1N1B4_849at 978.182 kb on + strand, within Pf1N1B4_849at 978.182 kb on + strand, within Pf1N1B4_849at 978.182 kb on + strand, within Pf1N1B4_849at 978.182 kb on + strand, within Pf1N1B4_849at 978.182 kb on + strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.183 kb on - strand, within Pf1N1B4_849at 978.187 kb on - strand, within Pf1N1B4_849at 978.267 kb on + strand, within Pf1N1B4_849at 978.268 kb on - strandat 978.392 kb on + strand, within Pf1N1B4_849at 978.392 kb on + strand, within Pf1N1B4_849at 978.392 kb on + strand, within Pf1N1B4_849at 978.392 kb on + strand, within Pf1N1B4_849at 978.393 kb on - strand, within Pf1N1B4_849at 978.393 kb on - strand, within Pf1N1B4_849at 978.393 kb on - strand, within Pf1N1B4_849at 978.396 kb on + strand, within Pf1N1B4_849at 978.397 kb on - strand, within Pf1N1B4_849at 978.397 kb on - strand, within Pf1N1B4_849at 978.402 kb on - strand, within Pf1N1B4_849at 978.461 kb on - strandat 978.720 kb on + strandat 978.720 kb on + strandat 978.721 kb on - strandat 978.744 kb on + strandat 978.745 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (N)
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976,988 - Pf1N1B4_848 0.20 +0.2
976,989 + Pf1N1B4_848 0.20 -1.2
976,990 - Pf1N1B4_848 0.20 -1.4
976,990 - Pf1N1B4_848 0.20 +2.0
976,990 - Pf1N1B4_848 0.20 -1.6
976,990 - Pf1N1B4_848 0.20 +1.0
977,037 + Pf1N1B4_848 0.26 +0.3
977,037 + +0.0
977,038 - Pf1N1B4_848 0.26 -0.4
977,038 - Pf1N1B4_848 0.26 -0.9
977,038 - Pf1N1B4_848 0.26 -0.3
977,038 - Pf1N1B4_848 0.26 -1.1
977,038 - Pf1N1B4_848 0.26 -1.6
977,116 - Pf1N1B4_848 0.36 +0.0
977,173 - Pf1N1B4_848 0.43 -0.6
977,283 + Pf1N1B4_848 0.57 +1.6
977,284 - Pf1N1B4_848 0.57 +1.0
977,410 + Pf1N1B4_848 0.73 -1.8
977,410 + Pf1N1B4_848 0.73 +0.0
977,411 - Pf1N1B4_848 0.73 +0.4
977,620 + +0.0
977,621 - -1.6
977,621 - -0.4
977,621 - -1.0
977,705 + +0.0
977,705 + +0.0
977,705 + -1.0
977,705 + +0.0
977,816 + Pf1N1B4_849 0.18 -0.4
977,816 + Pf1N1B4_849 0.18 -0.6
977,816 + +0.0
977,816 + Pf1N1B4_849 0.18 -0.3
977,816 + Pf1N1B4_849 0.18 -0.4
977,816 + Pf1N1B4_849 0.18 -0.8
977,816 + Pf1N1B4_849 0.18 +0.0
977,816 + Pf1N1B4_849 0.18 -0.3
977,816 + Pf1N1B4_849 0.18 +0.3
977,816 + Pf1N1B4_849 0.18 +1.0
977,817 - Pf1N1B4_849 0.18 +0.5
977,817 - Pf1N1B4_849 0.18 -0.6
977,817 - Pf1N1B4_849 0.18 +1.3
977,821 - Pf1N1B4_849 0.19 -0.6
977,891 + Pf1N1B4_849 0.25 -0.6
977,891 + Pf1N1B4_849 0.25 -1.0
977,891 + Pf1N1B4_849 0.25 +0.0
977,892 - Pf1N1B4_849 0.25 +2.0
977,892 - Pf1N1B4_849 0.25 +1.6
977,892 - Pf1N1B4_849 0.25 -1.3
977,936 + Pf1N1B4_849 0.29 -2.6
977,937 - Pf1N1B4_849 0.29 +0.0
977,937 - Pf1N1B4_849 0.29 +0.6
977,937 - Pf1N1B4_849 0.29 -0.5
977,937 - Pf1N1B4_849 0.29 +0.6
977,951 + Pf1N1B4_849 0.30 -0.4
977,952 - Pf1N1B4_849 0.30 -0.7
977,952 - Pf1N1B4_849 0.30 +0.0
977,984 + Pf1N1B4_849 0.33 -2.3
977,984 + Pf1N1B4_849 0.33 +1.6
977,985 - Pf1N1B4_849 0.33 +0.0
977,985 - Pf1N1B4_849 0.33 -2.6
978,036 - Pf1N1B4_849 0.37 +0.0
978,062 + Pf1N1B4_849 0.40 +0.0
978,062 + Pf1N1B4_849 0.40 -1.3
978,063 - Pf1N1B4_849 0.40 -2.3
978,063 - Pf1N1B4_849 0.40 +0.0
978,122 - Pf1N1B4_849 0.45 -0.6
978,137 - Pf1N1B4_849 0.46 -0.6
978,137 - Pf1N1B4_849 0.46 -0.6
978,137 - Pf1N1B4_849 0.46 -1.6
978,148 + Pf1N1B4_849 0.47 -0.3
978,162 - +1.0
978,182 + -2.0
978,182 + +0.0
978,182 + Pf1N1B4_849 0.50 -2.8
978,182 + Pf1N1B4_849 0.50 +0.6
978,182 + Pf1N1B4_849 0.50 -1.4
978,182 + Pf1N1B4_849 0.50 +0.3
978,182 + Pf1N1B4_849 0.50 -1.6
978,182 + Pf1N1B4_849 0.50 -2.6
978,182 + Pf1N1B4_849 0.50 +0.6
978,182 + Pf1N1B4_849 0.50 +0.5
978,183 - Pf1N1B4_849 0.50 +2.0
978,183 - Pf1N1B4_849 0.50 -1.6
978,183 - Pf1N1B4_849 0.50 +1.3
978,183 - Pf1N1B4_849 0.50 -0.7
978,183 - Pf1N1B4_849 0.50 -0.6
978,183 - Pf1N1B4_849 0.50 +0.0
978,183 - Pf1N1B4_849 0.50 -2.0
978,183 - Pf1N1B4_849 0.50 -0.7
978,183 - Pf1N1B4_849 0.50 -1.0
978,183 - Pf1N1B4_849 0.50 +0.0
978,183 - Pf1N1B4_849 0.50 +0.6
978,187 - Pf1N1B4_849 0.50 +0.6
978,267 + Pf1N1B4_849 0.57 -1.3
978,268 - +0.0
978,392 + Pf1N1B4_849 0.68 +0.0
978,392 + Pf1N1B4_849 0.68 +1.0
978,392 + Pf1N1B4_849 0.68 -1.1
978,392 + Pf1N1B4_849 0.68 -0.7
978,393 - Pf1N1B4_849 0.68 +0.6
978,393 - Pf1N1B4_849 0.68 +1.0
978,393 - Pf1N1B4_849 0.68 +1.0
978,396 + Pf1N1B4_849 0.69 -0.4
978,397 - Pf1N1B4_849 0.69 -1.0
978,397 - Pf1N1B4_849 0.69 -1.0
978,402 - Pf1N1B4_849 0.69 +0.0
978,461 - +0.7
978,720 + +0.3
978,720 + -1.0
978,721 - -1.0
978,744 + +0.3
978,745 - -0.4

Or see this region's nucleotide sequence