Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_847 and Pf1N1B4_848 are separated by 164 nucleotides Pf1N1B4_848 and Pf1N1B4_849 overlap by 13 nucleotides Pf1N1B4_849 and Pf1N1B4_850 are separated by 151 nucleotides
Pf1N1B4_847 - 2-isopropylmalate synthase (EC 2.3.3.13), at 974,985 to 976,664
_847
Pf1N1B4_848 - Aliphatic amidase AmiE (EC 3.5.1.4), at 976,829 to 977,620
_848
Pf1N1B4_849 - Aspartate aminotransferase (EC 2.6.1.1), at 977,608 to 978,756
_849
Pf1N1B4_850 - GTP-binding protein EngA, at 978,908 to 980,377
_850
Position (kb)
977
978
979 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 976.988 kb on - strand, within Pf1N1B4_848 at 976.989 kb on + strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 977.037 kb on + strand, within Pf1N1B4_848 at 977.037 kb on + strand at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.116 kb on - strand, within Pf1N1B4_848 at 977.173 kb on - strand, within Pf1N1B4_848 at 977.283 kb on + strand, within Pf1N1B4_848 at 977.284 kb on - strand, within Pf1N1B4_848 at 977.410 kb on + strand, within Pf1N1B4_848 at 977.410 kb on + strand, within Pf1N1B4_848 at 977.411 kb on - strand, within Pf1N1B4_848 at 977.620 kb on + strand at 977.621 kb on - strand at 977.621 kb on - strand at 977.621 kb on - strand at 977.705 kb on + strand at 977.705 kb on + strand at 977.705 kb on + strand at 977.705 kb on + strand at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.817 kb on - strand, within Pf1N1B4_849 at 977.817 kb on - strand, within Pf1N1B4_849 at 977.817 kb on - strand, within Pf1N1B4_849 at 977.821 kb on - strand, within Pf1N1B4_849 at 977.891 kb on + strand, within Pf1N1B4_849 at 977.891 kb on + strand, within Pf1N1B4_849 at 977.891 kb on + strand, within Pf1N1B4_849 at 977.892 kb on - strand, within Pf1N1B4_849 at 977.892 kb on - strand, within Pf1N1B4_849 at 977.892 kb on - strand, within Pf1N1B4_849 at 977.936 kb on + strand, within Pf1N1B4_849 at 977.937 kb on - strand, within Pf1N1B4_849 at 977.937 kb on - strand, within Pf1N1B4_849 at 977.937 kb on - strand, within Pf1N1B4_849 at 977.937 kb on - strand, within Pf1N1B4_849 at 977.951 kb on + strand, within Pf1N1B4_849 at 977.952 kb on - strand, within Pf1N1B4_849 at 977.952 kb on - strand, within Pf1N1B4_849 at 977.984 kb on + strand, within Pf1N1B4_849 at 977.984 kb on + strand, within Pf1N1B4_849 at 977.985 kb on - strand, within Pf1N1B4_849 at 977.985 kb on - strand, within Pf1N1B4_849 at 978.036 kb on - strand, within Pf1N1B4_849 at 978.062 kb on + strand, within Pf1N1B4_849 at 978.062 kb on + strand, within Pf1N1B4_849 at 978.063 kb on - strand, within Pf1N1B4_849 at 978.063 kb on - strand, within Pf1N1B4_849 at 978.122 kb on - strand, within Pf1N1B4_849 at 978.137 kb on - strand, within Pf1N1B4_849 at 978.137 kb on - strand, within Pf1N1B4_849 at 978.137 kb on - strand, within Pf1N1B4_849 at 978.148 kb on + strand, within Pf1N1B4_849 at 978.162 kb on - strand at 978.182 kb on + strand at 978.182 kb on + strand at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.187 kb on - strand, within Pf1N1B4_849 at 978.267 kb on + strand, within Pf1N1B4_849 at 978.268 kb on - strand at 978.392 kb on + strand, within Pf1N1B4_849 at 978.392 kb on + strand, within Pf1N1B4_849 at 978.392 kb on + strand, within Pf1N1B4_849 at 978.392 kb on + strand, within Pf1N1B4_849 at 978.393 kb on - strand, within Pf1N1B4_849 at 978.393 kb on - strand, within Pf1N1B4_849 at 978.393 kb on - strand, within Pf1N1B4_849 at 978.396 kb on + strand, within Pf1N1B4_849 at 978.397 kb on - strand, within Pf1N1B4_849 at 978.397 kb on - strand, within Pf1N1B4_849 at 978.402 kb on - strand, within Pf1N1B4_849 at 978.461 kb on - strand at 978.720 kb on + strand at 978.720 kb on + strand at 978.721 kb on - strand at 978.744 kb on + strand at 978.745 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 976,988 - Pf1N1B4_848 0.20 +0.2 976,989 + Pf1N1B4_848 0.20 -1.2 976,990 - Pf1N1B4_848 0.20 -1.4 976,990 - Pf1N1B4_848 0.20 +2.0 976,990 - Pf1N1B4_848 0.20 -1.6 976,990 - Pf1N1B4_848 0.20 +1.0 977,037 + Pf1N1B4_848 0.26 +0.3 977,037 + +0.0 977,038 - Pf1N1B4_848 0.26 -0.4 977,038 - Pf1N1B4_848 0.26 -0.9 977,038 - Pf1N1B4_848 0.26 -0.3 977,038 - Pf1N1B4_848 0.26 -1.1 977,038 - Pf1N1B4_848 0.26 -1.6 977,116 - Pf1N1B4_848 0.36 +0.0 977,173 - Pf1N1B4_848 0.43 -0.6 977,283 + Pf1N1B4_848 0.57 +1.6 977,284 - Pf1N1B4_848 0.57 +1.0 977,410 + Pf1N1B4_848 0.73 -1.8 977,410 + Pf1N1B4_848 0.73 +0.0 977,411 - Pf1N1B4_848 0.73 +0.4 977,620 + +0.0 977,621 - -1.6 977,621 - -0.4 977,621 - -1.0 977,705 + +0.0 977,705 + +0.0 977,705 + -1.0 977,705 + +0.0 977,816 + Pf1N1B4_849 0.18 -0.4 977,816 + Pf1N1B4_849 0.18 -0.6 977,816 + +0.0 977,816 + Pf1N1B4_849 0.18 -0.3 977,816 + Pf1N1B4_849 0.18 -0.4 977,816 + Pf1N1B4_849 0.18 -0.8 977,816 + Pf1N1B4_849 0.18 +0.0 977,816 + Pf1N1B4_849 0.18 -0.3 977,816 + Pf1N1B4_849 0.18 +0.3 977,816 + Pf1N1B4_849 0.18 +1.0 977,817 - Pf1N1B4_849 0.18 +0.5 977,817 - Pf1N1B4_849 0.18 -0.6 977,817 - Pf1N1B4_849 0.18 +1.3 977,821 - Pf1N1B4_849 0.19 -0.6 977,891 + Pf1N1B4_849 0.25 -0.6 977,891 + Pf1N1B4_849 0.25 -1.0 977,891 + Pf1N1B4_849 0.25 +0.0 977,892 - Pf1N1B4_849 0.25 +2.0 977,892 - Pf1N1B4_849 0.25 +1.6 977,892 - Pf1N1B4_849 0.25 -1.3 977,936 + Pf1N1B4_849 0.29 -2.6 977,937 - Pf1N1B4_849 0.29 +0.0 977,937 - Pf1N1B4_849 0.29 +0.6 977,937 - Pf1N1B4_849 0.29 -0.5 977,937 - Pf1N1B4_849 0.29 +0.6 977,951 + Pf1N1B4_849 0.30 -0.4 977,952 - Pf1N1B4_849 0.30 -0.7 977,952 - Pf1N1B4_849 0.30 +0.0 977,984 + Pf1N1B4_849 0.33 -2.3 977,984 + Pf1N1B4_849 0.33 +1.6 977,985 - Pf1N1B4_849 0.33 +0.0 977,985 - Pf1N1B4_849 0.33 -2.6 978,036 - Pf1N1B4_849 0.37 +0.0 978,062 + Pf1N1B4_849 0.40 +0.0 978,062 + Pf1N1B4_849 0.40 -1.3 978,063 - Pf1N1B4_849 0.40 -2.3 978,063 - Pf1N1B4_849 0.40 +0.0 978,122 - Pf1N1B4_849 0.45 -0.6 978,137 - Pf1N1B4_849 0.46 -0.6 978,137 - Pf1N1B4_849 0.46 -0.6 978,137 - Pf1N1B4_849 0.46 -1.6 978,148 + Pf1N1B4_849 0.47 -0.3 978,162 - +1.0 978,182 + -2.0 978,182 + +0.0 978,182 + Pf1N1B4_849 0.50 -2.8 978,182 + Pf1N1B4_849 0.50 +0.6 978,182 + Pf1N1B4_849 0.50 -1.4 978,182 + Pf1N1B4_849 0.50 +0.3 978,182 + Pf1N1B4_849 0.50 -1.6 978,182 + Pf1N1B4_849 0.50 -2.6 978,182 + Pf1N1B4_849 0.50 +0.6 978,182 + Pf1N1B4_849 0.50 +0.5 978,183 - Pf1N1B4_849 0.50 +2.0 978,183 - Pf1N1B4_849 0.50 -1.6 978,183 - Pf1N1B4_849 0.50 +1.3 978,183 - Pf1N1B4_849 0.50 -0.7 978,183 - Pf1N1B4_849 0.50 -0.6 978,183 - Pf1N1B4_849 0.50 +0.0 978,183 - Pf1N1B4_849 0.50 -2.0 978,183 - Pf1N1B4_849 0.50 -0.7 978,183 - Pf1N1B4_849 0.50 -1.0 978,183 - Pf1N1B4_849 0.50 +0.0 978,183 - Pf1N1B4_849 0.50 +0.6 978,187 - Pf1N1B4_849 0.50 +0.6 978,267 + Pf1N1B4_849 0.57 -1.3 978,268 - +0.0 978,392 + Pf1N1B4_849 0.68 +0.0 978,392 + Pf1N1B4_849 0.68 +1.0 978,392 + Pf1N1B4_849 0.68 -1.1 978,392 + Pf1N1B4_849 0.68 -0.7 978,393 - Pf1N1B4_849 0.68 +0.6 978,393 - Pf1N1B4_849 0.68 +1.0 978,393 - Pf1N1B4_849 0.68 +1.0 978,396 + Pf1N1B4_849 0.69 -0.4 978,397 - Pf1N1B4_849 0.69 -1.0 978,397 - Pf1N1B4_849 0.69 -1.0 978,402 - Pf1N1B4_849 0.69 +0.0 978,461 - +0.7 978,720 + +0.3 978,720 + -1.0 978,721 - -1.0 978,744 + +0.3 978,745 - -0.4
Or see this region's nucleotide sequence