Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5999

Experiment: L-Leucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5998 and Pf1N1B4_5999 are separated by 230 nucleotidesPf1N1B4_5999 and Pf1N1B4_6000 are separated by 111 nucleotides Pf1N1B4_5998 - Quinone oxidoreductase (EC 1.6.5.5), at 449,129 to 450,109 _5998 Pf1N1B4_5999 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 450,340 to 451,842 _5999 Pf1N1B4_6000 - Putative L-xylulose-5-phosphate 3-epimerase, at 451,954 to 453,024 _6000 Position (kb) 450 451 452Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 449.532 kb on + strandat 449.532 kb on + strand, within Pf1N1B4_5998at 449.533 kb on - strand, within Pf1N1B4_5998at 449.533 kb on - strand, within Pf1N1B4_5998at 449.533 kb on - strand, within Pf1N1B4_5998at 449.533 kb on - strand, within Pf1N1B4_5998at 449.718 kb on + strand, within Pf1N1B4_5998at 449.824 kb on - strand, within Pf1N1B4_5998at 449.836 kb on + strand, within Pf1N1B4_5998at 449.837 kb on - strand, within Pf1N1B4_5998at 449.837 kb on - strand, within Pf1N1B4_5998at 449.853 kb on + strandat 449.854 kb on - strand, within Pf1N1B4_5998at 449.876 kb on + strand, within Pf1N1B4_5998at 449.876 kb on + strandat 449.876 kb on + strand, within Pf1N1B4_5998at 449.876 kb on + strand, within Pf1N1B4_5998at 449.876 kb on + strand, within Pf1N1B4_5998at 449.876 kb on + strandat 449.877 kb on - strand, within Pf1N1B4_5998at 449.973 kb on + strand, within Pf1N1B4_5998at 449.973 kb on + strand, within Pf1N1B4_5998at 449.974 kb on - strand, within Pf1N1B4_5998at 449.974 kb on - strand, within Pf1N1B4_5998at 449.974 kb on - strand, within Pf1N1B4_5998at 450.169 kb on + strandat 450.169 kb on + strandat 450.264 kb on - strandat 450.338 kb on + strandat 450.338 kb on + strandat 450.338 kb on + strandat 450.338 kb on + strandat 450.338 kb on + strandat 450.339 kb on - strandat 450.339 kb on - strandat 450.418 kb on + strandat 450.418 kb on + strandat 450.589 kb on + strand, within Pf1N1B4_5999at 450.589 kb on + strand, within Pf1N1B4_5999at 450.589 kb on + strand, within Pf1N1B4_5999at 450.590 kb on - strand, within Pf1N1B4_5999at 450.590 kb on - strand, within Pf1N1B4_5999at 450.590 kb on - strand, within Pf1N1B4_5999at 450.590 kb on - strand, within Pf1N1B4_5999at 450.590 kb on - strand, within Pf1N1B4_5999at 450.590 kb on - strand, within Pf1N1B4_5999at 450.712 kb on + strandat 450.713 kb on - strand, within Pf1N1B4_5999at 450.762 kb on + strand, within Pf1N1B4_5999at 450.762 kb on + strand, within Pf1N1B4_5999at 450.762 kb on + strand, within Pf1N1B4_5999at 450.762 kb on + strand, within Pf1N1B4_5999at 450.766 kb on + strand, within Pf1N1B4_5999at 450.766 kb on + strand, within Pf1N1B4_5999at 450.892 kb on + strand, within Pf1N1B4_5999at 450.984 kb on - strand, within Pf1N1B4_5999at 451.007 kb on + strand, within Pf1N1B4_5999at 451.008 kb on - strand, within Pf1N1B4_5999at 451.105 kb on - strand, within Pf1N1B4_5999at 451.192 kb on + strand, within Pf1N1B4_5999at 451.192 kb on + strand, within Pf1N1B4_5999at 451.192 kb on + strand, within Pf1N1B4_5999at 451.304 kb on - strand, within Pf1N1B4_5999at 451.334 kb on - strand, within Pf1N1B4_5999at 451.334 kb on - strand, within Pf1N1B4_5999at 451.371 kb on - strand, within Pf1N1B4_5999at 451.382 kb on - strand, within Pf1N1B4_5999at 451.382 kb on - strand, within Pf1N1B4_5999at 451.531 kb on + strand, within Pf1N1B4_5999at 451.696 kb on + strandat 451.696 kb on + strandat 451.697 kb on - strandat 451.697 kb on - strandat 451.697 kb on - strandat 451.697 kb on - strandat 451.847 kb on - strandat 451.861 kb on - strandat 451.861 kb on - strandat 451.868 kb on - strandat 451.868 kb on - strandat 451.868 kb on - strandat 451.883 kb on + strandat 451.884 kb on - strandat 451.884 kb on - strandat 451.962 kb on + strandat 451.963 kb on - strandat 451.963 kb on - strandat 452.431 kb on + strand, within Pf1N1B4_6000at 452.624 kb on + strand, within Pf1N1B4_6000

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (N)
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449,532 + -0.2
449,532 + Pf1N1B4_5998 0.41 +0.0
449,533 - Pf1N1B4_5998 0.41 -1.8
449,533 - Pf1N1B4_5998 0.41 -0.3
449,533 - Pf1N1B4_5998 0.41 +0.4
449,533 - Pf1N1B4_5998 0.41 -0.2
449,718 + Pf1N1B4_5998 0.60 +1.0
449,824 - Pf1N1B4_5998 0.71 +0.1
449,836 + Pf1N1B4_5998 0.72 +1.0
449,837 - Pf1N1B4_5998 0.72 +0.5
449,837 - Pf1N1B4_5998 0.72 -0.2
449,853 + -0.2
449,854 - Pf1N1B4_5998 0.74 -1.1
449,876 + Pf1N1B4_5998 0.76 +0.4
449,876 + -0.8
449,876 + Pf1N1B4_5998 0.76 +1.1
449,876 + Pf1N1B4_5998 0.76 -3.0
449,876 + Pf1N1B4_5998 0.76 -1.2
449,876 + +0.8
449,877 - Pf1N1B4_5998 0.76 -0.8
449,973 + Pf1N1B4_5998 0.86 +0.4
449,973 + Pf1N1B4_5998 0.86 +1.0
449,974 - Pf1N1B4_5998 0.86 +1.0
449,974 - Pf1N1B4_5998 0.86 -0.6
449,974 - Pf1N1B4_5998 0.86 -2.2
450,169 + +0.7
450,169 + +1.1
450,264 - +1.4
450,338 + -0.9
450,338 + -0.6
450,338 + -0.8
450,338 + -0.2
450,338 + +1.4
450,339 - -0.2
450,339 - -1.8
450,418 + -0.6
450,418 + -1.2
450,589 + Pf1N1B4_5999 0.17 -0.9
450,589 + Pf1N1B4_5999 0.17 -0.0
450,589 + Pf1N1B4_5999 0.17 -0.2
450,590 - Pf1N1B4_5999 0.17 +0.4
450,590 - Pf1N1B4_5999 0.17 -1.8
450,590 - Pf1N1B4_5999 0.17 -0.2
450,590 - Pf1N1B4_5999 0.17 -1.8
450,590 - Pf1N1B4_5999 0.17 -0.2
450,590 - Pf1N1B4_5999 0.17 +1.3
450,712 + -0.2
450,713 - Pf1N1B4_5999 0.25 +0.8
450,762 + Pf1N1B4_5999 0.28 -0.8
450,762 + Pf1N1B4_5999 0.28 -0.2
450,762 + Pf1N1B4_5999 0.28 -1.2
450,762 + Pf1N1B4_5999 0.28 -2.2
450,766 + Pf1N1B4_5999 0.28 +0.6
450,766 + Pf1N1B4_5999 0.28 +0.8
450,892 + Pf1N1B4_5999 0.37 -0.2
450,984 - Pf1N1B4_5999 0.43 -0.5
451,007 + Pf1N1B4_5999 0.44 -2.2
451,008 - Pf1N1B4_5999 0.44 -1.4
451,105 - Pf1N1B4_5999 0.51 -0.2
451,192 + Pf1N1B4_5999 0.57 +1.4
451,192 + Pf1N1B4_5999 0.57 +0.2
451,192 + Pf1N1B4_5999 0.57 -1.6
451,304 - Pf1N1B4_5999 0.64 -0.6
451,334 - Pf1N1B4_5999 0.66 -1.2
451,334 - Pf1N1B4_5999 0.66 -0.2
451,371 - Pf1N1B4_5999 0.69 -0.2
451,382 - Pf1N1B4_5999 0.69 -1.8
451,382 - Pf1N1B4_5999 0.69 -1.8
451,531 + Pf1N1B4_5999 0.79 +0.1
451,696 + -2.2
451,696 + +0.3
451,697 - -1.5
451,697 - -0.5
451,697 - -0.2
451,697 - -1.2
451,847 - -1.2
451,861 - -2.2
451,861 - +1.8
451,868 - +0.8
451,868 - +0.2
451,868 - +0.8
451,883 + -0.3
451,884 - -1.2
451,884 - -0.8
451,962 + +0.8
451,963 - -1.2
451,963 - -1.8
452,431 + Pf1N1B4_6000 0.45 +0.8
452,624 + Pf1N1B4_6000 0.63 -0.5

Or see this region's nucleotide sequence