Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4871 and Pf1N1B4_4872 are separated by 80 nucleotides Pf1N1B4_4872 and Pf1N1B4_4873 are separated by 37 nucleotides Pf1N1B4_4873 and Pf1N1B4_4874 are separated by 258 nucleotides Pf1N1B4_4874 and Pf1N1B4_4875 are separated by 274 nucleotides
Pf1N1B4_4871 - Aldo-keto reductase, at 5,391,641 to 5,391,895
_4871
Pf1N1B4_4872 - Short-chain dehydrogenase/reductase SDR, at 5,391,976 to 5,392,818
_4872
Pf1N1B4_4873 - clavaldehyde dehydrogenase, at 5,392,856 to 5,393,617
_4873
Pf1N1B4_4874 - hypothetical protein, at 5,393,876 to 5,394,127
_4874
Pf1N1B4_4875 - Msl4750 protein, at 5,394,402 to 5,394,779
_4875
Position (kb) 5392
5393
5394 Strain fitness (log2 ratio) -2
-1
0
1 at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.948 kb on - strand at 5391.951 kb on + strand at 5392.028 kb on + strand at 5392.028 kb on + strand at 5392.490 kb on + strand, within Pf1N1B4_4872 at 5392.555 kb on + strand, within Pf1N1B4_4872 at 5392.556 kb on - strand, within Pf1N1B4_4872 at 5392.579 kb on + strand, within Pf1N1B4_4872 at 5392.580 kb on - strand, within Pf1N1B4_4872 at 5392.782 kb on + strand at 5392.782 kb on + strand at 5393.002 kb on - strand, within Pf1N1B4_4873 at 5393.153 kb on - strand, within Pf1N1B4_4873 at 5393.216 kb on + strand at 5393.216 kb on + strand at 5393.216 kb on + strand, within Pf1N1B4_4873 at 5393.217 kb on - strand, within Pf1N1B4_4873 at 5393.316 kb on + strand, within Pf1N1B4_4873 at 5393.330 kb on + strand at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.340 kb on - strand, within Pf1N1B4_4873 at 5393.498 kb on + strand, within Pf1N1B4_4873 at 5393.550 kb on + strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.616 kb on - strand at 5393.679 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.794 kb on - strand at 5393.900 kb on - strand at 5393.927 kb on - strand, within Pf1N1B4_4874 at 5394.013 kb on + strand, within Pf1N1B4_4874 at 5394.060 kb on + strand, within Pf1N1B4_4874 at 5394.060 kb on + strand, within Pf1N1B4_4874 at 5394.125 kb on + strand at 5394.267 kb on + strand at 5394.311 kb on + strand at 5394.311 kb on + strand at 5394.311 kb on + strand at 5394.311 kb on + strand at 5394.312 kb on - strand at 5394.312 kb on - strand at 5394.312 kb on - strand at 5394.312 kb on - strand at 5394.376 kb on + strand at 5394.398 kb on + strand at 5394.398 kb on + strand at 5394.398 kb on + strand at 5394.399 kb on - strand at 5394.399 kb on - strand at 5394.399 kb on - strand at 5394.489 kb on + strand, within Pf1N1B4_4875 at 5394.489 kb on + strand, within Pf1N1B4_4875 at 5394.490 kb on - strand, within Pf1N1B4_4875 at 5394.504 kb on + strand, within Pf1N1B4_4875 at 5394.505 kb on - strand, within Pf1N1B4_4875 at 5394.537 kb on + strand at 5394.537 kb on + strand, within Pf1N1B4_4875 at 5394.537 kb on + strand, within Pf1N1B4_4875 at 5394.537 kb on + strand, within Pf1N1B4_4875 at 5394.538 kb on - strand, within Pf1N1B4_4875 at 5394.570 kb on + strand, within Pf1N1B4_4875 at 5394.570 kb on + strand, within Pf1N1B4_4875 at 5394.571 kb on - strand, within Pf1N1B4_4875
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 5,391,947 + -1.3 5,391,947 + -0.7 5,391,947 + -1.2 5,391,947 + -0.1 5,391,947 + -0.9 5,391,948 - -2.0 5,391,951 + -2.0 5,392,028 + -0.0 5,392,028 + -2.0 5,392,490 + Pf1N1B4_4872 0.61 +1.2 5,392,555 + Pf1N1B4_4872 0.69 +0.2 5,392,556 - Pf1N1B4_4872 0.69 +0.2 5,392,579 + Pf1N1B4_4872 0.72 -0.9 5,392,580 - Pf1N1B4_4872 0.72 -0.1 5,392,782 + -0.4 5,392,782 + -0.0 5,393,002 - Pf1N1B4_4873 0.19 +0.6 5,393,153 - Pf1N1B4_4873 0.39 -0.0 5,393,216 + -2.0 5,393,216 + +0.2 5,393,216 + Pf1N1B4_4873 0.47 -0.1 5,393,217 - Pf1N1B4_4873 0.47 +0.9 5,393,316 + Pf1N1B4_4873 0.60 +1.2 5,393,330 + +1.6 5,393,331 - Pf1N1B4_4873 0.62 -0.8 5,393,331 - Pf1N1B4_4873 0.62 +0.2 5,393,340 - Pf1N1B4_4873 0.64 -0.0 5,393,498 + Pf1N1B4_4873 0.84 +0.9 5,393,550 + -0.4 5,393,550 - -0.0 5,393,550 - -1.4 5,393,550 - -0.4 5,393,550 - -1.0 5,393,616 - -0.7 5,393,679 + -0.6 5,393,694 + -1.0 5,393,694 + -0.8 5,393,694 + +0.6 5,393,694 + +0.1 5,393,694 + -0.4 5,393,695 - -0.5 5,393,695 - -0.4 5,393,695 - +0.9 5,393,695 - -0.4 5,393,695 - -2.0 5,393,695 - -0.4 5,393,794 - -0.2 5,393,900 - +0.6 5,393,927 - Pf1N1B4_4874 0.20 -0.4 5,394,013 + Pf1N1B4_4874 0.54 -2.4 5,394,060 + Pf1N1B4_4874 0.73 -0.4 5,394,060 + Pf1N1B4_4874 0.73 +0.6 5,394,125 + -1.4 5,394,267 + -1.0 5,394,311 + +0.3 5,394,311 + +0.2 5,394,311 + -0.4 5,394,311 + +1.2 5,394,312 - +1.6 5,394,312 - +0.2 5,394,312 - -0.4 5,394,312 - -0.4 5,394,376 + -0.4 5,394,398 + +1.2 5,394,398 + -0.2 5,394,398 + +1.2 5,394,399 - -0.9 5,394,399 - -0.0 5,394,399 - +0.3 5,394,489 + Pf1N1B4_4875 0.23 +0.3 5,394,489 + Pf1N1B4_4875 0.23 -0.7 5,394,490 - Pf1N1B4_4875 0.23 -1.0 5,394,504 + Pf1N1B4_4875 0.27 -0.4 5,394,505 - Pf1N1B4_4875 0.27 -0.7 5,394,537 + +0.2 5,394,537 + Pf1N1B4_4875 0.36 +0.6 5,394,537 + Pf1N1B4_4875 0.36 -0.4 5,394,537 + Pf1N1B4_4875 0.36 -1.7 5,394,538 - Pf1N1B4_4875 0.36 +1.2 5,394,570 + Pf1N1B4_4875 0.44 -0.4 5,394,570 + Pf1N1B4_4875 0.44 -0.8 5,394,571 - Pf1N1B4_4875 0.45 -1.0
Or see this region's nucleotide sequence