Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4869 and Pf1N1B4_4870 are separated by 12 nucleotides Pf1N1B4_4870 and Pf1N1B4_4871 are separated by 109 nucleotides Pf1N1B4_4871 and Pf1N1B4_4872 are separated by 80 nucleotides Pf1N1B4_4872 and Pf1N1B4_4873 are separated by 37 nucleotides
Pf1N1B4_4869 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 5,388,805 to 5,391,045
_4869
Pf1N1B4_4870 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 5,391,058 to 5,391,531
_4870
Pf1N1B4_4871 - Aldo-keto reductase, at 5,391,641 to 5,391,895
_4871
Pf1N1B4_4872 - Short-chain dehydrogenase/reductase SDR, at 5,391,976 to 5,392,818
_4872
Pf1N1B4_4873 - clavaldehyde dehydrogenase, at 5,392,856 to 5,393,617
_4873
Position (kb) 5391
5392
5393 Strain fitness (log2 ratio) -2
-1
0
1 at 5390.995 kb on + strand at 5390.995 kb on + strand at 5390.995 kb on + strand at 5390.996 kb on - strand at 5390.996 kb on - strand at 5391.271 kb on + strand, within Pf1N1B4_4870 at 5391.271 kb on + strand at 5391.393 kb on + strand, within Pf1N1B4_4870 at 5391.394 kb on - strand, within Pf1N1B4_4870 at 5391.394 kb on - strand, within Pf1N1B4_4870 at 5391.487 kb on + strand at 5391.500 kb on + strand at 5391.501 kb on - strand at 5391.596 kb on + strand at 5391.597 kb on - strand at 5391.727 kb on + strand, within Pf1N1B4_4871 at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.948 kb on - strand at 5391.951 kb on + strand at 5392.028 kb on + strand at 5392.028 kb on + strand at 5392.490 kb on + strand, within Pf1N1B4_4872 at 5392.555 kb on + strand, within Pf1N1B4_4872 at 5392.556 kb on - strand, within Pf1N1B4_4872 at 5392.579 kb on + strand, within Pf1N1B4_4872 at 5392.580 kb on - strand, within Pf1N1B4_4872 at 5392.782 kb on + strand at 5392.782 kb on + strand at 5393.002 kb on - strand, within Pf1N1B4_4873 at 5393.153 kb on - strand, within Pf1N1B4_4873 at 5393.216 kb on + strand at 5393.216 kb on + strand at 5393.216 kb on + strand, within Pf1N1B4_4873 at 5393.217 kb on - strand, within Pf1N1B4_4873 at 5393.316 kb on + strand, within Pf1N1B4_4873 at 5393.330 kb on + strand at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.340 kb on - strand, within Pf1N1B4_4873 at 5393.498 kb on + strand, within Pf1N1B4_4873 at 5393.550 kb on + strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.616 kb on - strand at 5393.679 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 5,390,995 + +1.4 5,390,995 + +0.7 5,390,995 + +0.6 5,390,996 - -0.3 5,390,996 - -1.7 5,391,271 + Pf1N1B4_4870 0.45 -1.7 5,391,271 + -0.4 5,391,393 + Pf1N1B4_4870 0.71 -0.4 5,391,394 - Pf1N1B4_4870 0.71 -2.6 5,391,394 - Pf1N1B4_4870 0.71 -1.0 5,391,487 + +0.2 5,391,500 + +0.5 5,391,501 - -0.7 5,391,596 + -0.4 5,391,597 - -0.8 5,391,727 + Pf1N1B4_4871 0.34 -0.4 5,391,947 + -1.3 5,391,947 + -0.7 5,391,947 + -1.2 5,391,947 + -0.1 5,391,947 + -0.9 5,391,948 - -2.0 5,391,951 + -2.0 5,392,028 + -0.0 5,392,028 + -2.0 5,392,490 + Pf1N1B4_4872 0.61 +1.2 5,392,555 + Pf1N1B4_4872 0.69 +0.2 5,392,556 - Pf1N1B4_4872 0.69 +0.2 5,392,579 + Pf1N1B4_4872 0.72 -0.9 5,392,580 - Pf1N1B4_4872 0.72 -0.1 5,392,782 + -0.4 5,392,782 + -0.0 5,393,002 - Pf1N1B4_4873 0.19 +0.6 5,393,153 - Pf1N1B4_4873 0.39 -0.0 5,393,216 + -2.0 5,393,216 + +0.2 5,393,216 + Pf1N1B4_4873 0.47 -0.1 5,393,217 - Pf1N1B4_4873 0.47 +0.9 5,393,316 + Pf1N1B4_4873 0.60 +1.2 5,393,330 + +1.6 5,393,331 - Pf1N1B4_4873 0.62 -0.8 5,393,331 - Pf1N1B4_4873 0.62 +0.2 5,393,340 - Pf1N1B4_4873 0.64 -0.0 5,393,498 + Pf1N1B4_4873 0.84 +0.9 5,393,550 + -0.4 5,393,550 - -0.0 5,393,550 - -1.4 5,393,550 - -0.4 5,393,550 - -1.0 5,393,616 - -0.7 5,393,679 + -0.6 5,393,694 + -1.0 5,393,694 + -0.8 5,393,694 + +0.6 5,393,694 + +0.1 5,393,694 + -0.4 5,393,695 - -0.5 5,393,695 - -0.4 5,393,695 - +0.9 5,393,695 - -0.4 5,393,695 - -2.0 5,393,695 - -0.4 5,393,794 - -0.2
Or see this region's nucleotide sequence