Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4092 and Pf1N1B4_4093 are separated by 159 nucleotides Pf1N1B4_4093 and Pf1N1B4_4094 are separated by 225 nucleotides Pf1N1B4_4094 and Pf1N1B4_4095 are separated by 10 nucleotides
Pf1N1B4_4092 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 4,540,762 to 4,542,174
_4092
Pf1N1B4_4093 - Alkyl hydroperoxide reductase subunit C-like protein, at 4,542,334 to 4,542,987
_4093
Pf1N1B4_4094 - Uncharacterized conserved protein, at 4,543,213 to 4,543,710
_4094
Pf1N1B4_4095 - Transcriptional regulator, LacI family, at 4,543,721 to 4,544,722
_4095
Position (kb)
4542
4543 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.444 kb on - strand, within Pf1N1B4_4092 at 4541.630 kb on - strand, within Pf1N1B4_4092 at 4541.630 kb on - strand at 4541.833 kb on + strand at 4541.834 kb on - strand, within Pf1N1B4_4092 at 4541.894 kb on - strand, within Pf1N1B4_4092 at 4541.896 kb on + strand, within Pf1N1B4_4092 at 4541.896 kb on + strand, within Pf1N1B4_4092 at 4541.896 kb on + strand, within Pf1N1B4_4092 at 4541.897 kb on - strand, within Pf1N1B4_4092 at 4541.897 kb on - strand, within Pf1N1B4_4092 at 4541.969 kb on + strand, within Pf1N1B4_4092 at 4541.969 kb on + strand, within Pf1N1B4_4092 at 4541.986 kb on + strand, within Pf1N1B4_4092 at 4541.986 kb on + strand, within Pf1N1B4_4092 at 4542.072 kb on + strand at 4542.112 kb on + strand at 4542.115 kb on + strand at 4542.240 kb on + strand at 4542.240 kb on + strand at 4542.243 kb on - strand at 4542.307 kb on + strand at 4542.320 kb on + strand at 4542.320 kb on + strand at 4542.484 kb on + strand, within Pf1N1B4_4093 at 4542.656 kb on - strand, within Pf1N1B4_4093 at 4542.712 kb on - strand, within Pf1N1B4_4093 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.929 kb on - strand at 4542.958 kb on + strand at 4542.959 kb on - strand at 4543.021 kb on + strand at 4543.167 kb on + strand at 4543.167 kb on + strand at 4543.176 kb on - strand at 4543.176 kb on - strand at 4543.380 kb on - strand, within Pf1N1B4_4094 at 4543.445 kb on - strand, within Pf1N1B4_4094 at 4543.536 kb on - strand, within Pf1N1B4_4094 at 4543.928 kb on - strand, within Pf1N1B4_4095
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 4,541,423 - Pf1N1B4_4092 0.47 -1.8 4,541,423 - Pf1N1B4_4092 0.47 +0.8 4,541,423 - Pf1N1B4_4092 0.47 -0.1 4,541,423 - -2.6 4,541,423 - Pf1N1B4_4092 0.47 +0.2 4,541,444 - Pf1N1B4_4092 0.48 -1.8 4,541,630 - Pf1N1B4_4092 0.61 -1.2 4,541,630 - -0.2 4,541,833 + -0.2 4,541,834 - Pf1N1B4_4092 0.76 -0.2 4,541,894 - Pf1N1B4_4092 0.80 +0.2 4,541,896 + Pf1N1B4_4092 0.80 -0.7 4,541,896 + Pf1N1B4_4092 0.80 +0.2 4,541,896 + Pf1N1B4_4092 0.80 -1.2 4,541,897 - Pf1N1B4_4092 0.80 -2.2 4,541,897 - Pf1N1B4_4092 0.80 -1.2 4,541,969 + Pf1N1B4_4092 0.85 -0.2 4,541,969 + Pf1N1B4_4092 0.85 -0.6 4,541,986 + Pf1N1B4_4092 0.87 +0.1 4,541,986 + Pf1N1B4_4092 0.87 -0.8 4,542,072 + +1.0 4,542,112 + +0.8 4,542,115 + -0.8 4,542,240 + -0.2 4,542,240 + -0.0 4,542,243 - -1.2 4,542,307 + -0.9 4,542,320 + -0.2 4,542,320 + +1.9 4,542,484 + Pf1N1B4_4093 0.23 -0.7 4,542,656 - Pf1N1B4_4093 0.49 +0.8 4,542,712 - Pf1N1B4_4093 0.58 -1.6 4,542,770 - Pf1N1B4_4093 0.67 -0.5 4,542,770 - Pf1N1B4_4093 0.67 +0.8 4,542,929 - -1.2 4,542,958 + -0.2 4,542,959 - -2.2 4,543,021 + -2.2 4,543,167 + -0.2 4,543,167 + -1.8 4,543,176 - -0.8 4,543,176 - +0.8 4,543,380 - Pf1N1B4_4094 0.34 -0.2 4,543,445 - Pf1N1B4_4094 0.47 +1.1 4,543,536 - Pf1N1B4_4094 0.65 -1.4 4,543,928 - Pf1N1B4_4095 0.21 -0.2
Or see this region's nucleotide sequence