Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3779 and Pf1N1B4_3780 are separated by 143 nucleotides Pf1N1B4_3780 and Pf1N1B4_3781 are separated by 81 nucleotides
Pf1N1B4_3779 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 4,188,038 to 4,189,003
_3779
Pf1N1B4_3780 - Chorismate synthase (EC 4.2.3.5), at 4,189,147 to 4,190,238
_3780
Pf1N1B4_3781 - Nucleoside:H+ symporter:Major facilitator superfamily, at 4,190,320 to 4,191,465
_3781
Position (kb)
4189
4190
4191 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4188.476 kb on + strand, within Pf1N1B4_3779 at 4188.648 kb on + strand, within Pf1N1B4_3779 at 4188.648 kb on + strand, within Pf1N1B4_3779 at 4188.667 kb on + strand, within Pf1N1B4_3779 at 4188.668 kb on - strand, within Pf1N1B4_3779 at 4188.948 kb on + strand at 4188.973 kb on + strand at 4188.974 kb on - strand at 4188.974 kb on - strand at 4189.061 kb on + strand at 4189.061 kb on + strand at 4189.061 kb on + strand at 4189.079 kb on + strand at 4189.097 kb on - strand at 4189.097 kb on - strand at 4189.097 kb on - strand at 4189.098 kb on + strand at 4189.120 kb on + strand at 4189.120 kb on + strand at 4190.257 kb on + strand at 4190.257 kb on + strand at 4190.356 kb on + strand at 4190.356 kb on + strand at 4190.357 kb on - strand at 4190.357 kb on - strand at 4190.365 kb on + strand at 4190.365 kb on + strand at 4190.365 kb on + strand at 4190.408 kb on - strand at 4190.408 kb on - strand at 4190.408 kb on - strand at 4190.512 kb on + strand, within Pf1N1B4_3781 at 4190.512 kb on + strand, within Pf1N1B4_3781 at 4190.556 kb on + strand, within Pf1N1B4_3781 at 4190.557 kb on - strand, within Pf1N1B4_3781 at 4190.557 kb on - strand, within Pf1N1B4_3781 at 4190.557 kb on - strand, within Pf1N1B4_3781 at 4190.781 kb on + strand, within Pf1N1B4_3781 at 4190.782 kb on - strand, within Pf1N1B4_3781 at 4190.794 kb on + strand, within Pf1N1B4_3781 at 4190.794 kb on + strand, within Pf1N1B4_3781 at 4190.795 kb on - strand, within Pf1N1B4_3781 at 4190.935 kb on + strand, within Pf1N1B4_3781 at 4190.935 kb on + strand, within Pf1N1B4_3781 at 4190.936 kb on - strand at 4190.936 kb on - strand, within Pf1N1B4_3781 at 4190.936 kb on - strand, within Pf1N1B4_3781 at 4190.974 kb on + strand, within Pf1N1B4_3781 at 4190.974 kb on + strand, within Pf1N1B4_3781 at 4190.974 kb on + strand at 4190.974 kb on + strand at 4190.975 kb on - strand, within Pf1N1B4_3781 at 4190.975 kb on - strand, within Pf1N1B4_3781 at 4190.977 kb on + strand, within Pf1N1B4_3781 at 4190.977 kb on + strand, within Pf1N1B4_3781 at 4190.977 kb on + strand, within Pf1N1B4_3781 at 4190.977 kb on + strand at 4190.977 kb on + strand, within Pf1N1B4_3781 at 4190.978 kb on - strand, within Pf1N1B4_3781 at 4190.978 kb on - strand, within Pf1N1B4_3781 at 4191.033 kb on + strand at 4191.065 kb on + strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.066 kb on - strand, within Pf1N1B4_3781 at 4191.068 kb on + strand, within Pf1N1B4_3781
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 4,188,476 + Pf1N1B4_3779 0.45 -0.0 4,188,648 + Pf1N1B4_3779 0.63 +0.3 4,188,648 + Pf1N1B4_3779 0.63 -2.5 4,188,667 + Pf1N1B4_3779 0.65 +0.5 4,188,668 - Pf1N1B4_3779 0.65 -0.5 4,188,948 + +0.3 4,188,973 + +0.9 4,188,974 - -0.3 4,188,974 - -1.1 4,189,061 + +0.7 4,189,061 + -0.9 4,189,061 + +0.5 4,189,079 + -0.7 4,189,097 - -0.1 4,189,097 - -0.1 4,189,097 - +0.5 4,189,098 + -0.1 4,189,120 + -1.7 4,189,120 + +0.3 4,190,257 + -0.1 4,190,257 + -0.4 4,190,356 + +0.1 4,190,356 + +0.2 4,190,357 - +1.2 4,190,357 - +1.1 4,190,365 + -0.1 4,190,365 + -1.1 4,190,365 + -2.7 4,190,408 - -0.1 4,190,408 - -0.5 4,190,408 - +0.2 4,190,512 + Pf1N1B4_3781 0.17 -0.1 4,190,512 + Pf1N1B4_3781 0.17 -0.1 4,190,556 + Pf1N1B4_3781 0.21 -0.7 4,190,557 - Pf1N1B4_3781 0.21 -1.4 4,190,557 - Pf1N1B4_3781 0.21 -1.9 4,190,557 - Pf1N1B4_3781 0.21 +1.5 4,190,781 + Pf1N1B4_3781 0.40 +1.1 4,190,782 - Pf1N1B4_3781 0.40 -0.7 4,190,794 + Pf1N1B4_3781 0.41 -0.1 4,190,794 + Pf1N1B4_3781 0.41 +0.6 4,190,795 - Pf1N1B4_3781 0.41 +0.5 4,190,935 + Pf1N1B4_3781 0.54 +1.5 4,190,935 + Pf1N1B4_3781 0.54 -1.0 4,190,936 - -0.1 4,190,936 - Pf1N1B4_3781 0.54 +0.5 4,190,936 - Pf1N1B4_3781 0.54 +1.9 4,190,974 + Pf1N1B4_3781 0.57 +1.5 4,190,974 + Pf1N1B4_3781 0.57 -1.1 4,190,974 + -0.7 4,190,974 + +1.9 4,190,975 - Pf1N1B4_3781 0.57 +0.2 4,190,975 - Pf1N1B4_3781 0.57 +2.2 4,190,977 + Pf1N1B4_3781 0.57 +0.3 4,190,977 + Pf1N1B4_3781 0.57 +1.3 4,190,977 + Pf1N1B4_3781 0.57 -0.1 4,190,977 + +1.5 4,190,977 + Pf1N1B4_3781 0.57 +1.3 4,190,978 - Pf1N1B4_3781 0.57 +1.2 4,190,978 - Pf1N1B4_3781 0.57 -0.1 4,191,033 + +0.9 4,191,065 + Pf1N1B4_3781 0.65 -0.1 4,191,066 - Pf1N1B4_3781 0.65 -0.1 4,191,066 - Pf1N1B4_3781 0.65 -0.1 4,191,066 - Pf1N1B4_3781 0.65 -1.3 4,191,066 - Pf1N1B4_3781 0.65 +0.9 4,191,066 - Pf1N1B4_3781 0.65 +1.5 4,191,068 + Pf1N1B4_3781 0.65 -1.1
Or see this region's nucleotide sequence