Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3561 and Pf1N1B4_3562 are separated by 103 nucleotides Pf1N1B4_3562 and Pf1N1B4_3563 are separated by 75 nucleotides Pf1N1B4_3563 and Pf1N1B4_3564 are separated by 285 nucleotides Pf1N1B4_3564 and Pf1N1B4_3565 overlap by 1 nucleotides Pf1N1B4_3565 and Pf1N1B4_3566 are separated by 105 nucleotides
Pf1N1B4_3561 - Xanthine transporter,putative, at 3,965,101 to 3,966,300
_3561
Pf1N1B4_3562 - FIG00954951: hypothetical protein, at 3,966,404 to 3,966,586
_3562
Pf1N1B4_3563 - hypothetical protein, at 3,966,662 to 3,966,793
_3563
Pf1N1B4_3564 - Aspartate aminotransferase (EC 2.6.1.1), at 3,967,079 to 3,968,272
_3564
Pf1N1B4_3565 - Pterin-4-alpha-carbinolamine dehydratase (EC 4.2.1.96), at 3,968,272 to 3,968,628
_3565
Pf1N1B4_3566 - Phenylalanine-4-hydroxylase (EC 1.14.16.1), at 3,968,734 to 3,969,519
_3566
Position (kb)
3967
3968
3969 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3966.128 kb on + strand, within Pf1N1B4_3561 at 3966.128 kb on + strand, within Pf1N1B4_3561 at 3966.188 kb on + strand at 3966.188 kb on + strand at 3966.188 kb on + strand at 3966.199 kb on + strand at 3966.199 kb on + strand at 3966.199 kb on + strand at 3966.200 kb on - strand at 3966.200 kb on - strand at 3966.327 kb on + strand at 3966.328 kb on - strand at 3966.331 kb on + strand at 3966.331 kb on + strand at 3966.331 kb on + strand at 3966.332 kb on - strand at 3966.332 kb on - strand at 3966.332 kb on - strand at 3966.332 kb on - strand at 3966.332 kb on - strand at 3966.332 kb on - strand at 3966.332 kb on - strand at 3966.340 kb on + strand at 3966.340 kb on + strand at 3966.340 kb on + strand at 3966.341 kb on - strand at 3966.341 kb on - strand at 3966.341 kb on - strand at 3966.384 kb on + strand at 3966.385 kb on - strand at 3966.385 kb on - strand at 3966.624 kb on + strand at 3966.624 kb on + strand at 3966.624 kb on + strand at 3966.624 kb on + strand at 3966.624 kb on + strand at 3966.624 kb on + strand at 3966.624 kb on + strand at 3966.624 kb on + strand at 3966.627 kb on + strand at 3966.660 kb on + strand at 3966.778 kb on + strand, within Pf1N1B4_3563 at 3966.809 kb on - strand at 3966.820 kb on + strand at 3966.820 kb on + strand at 3966.821 kb on - strand at 3966.821 kb on - strand at 3966.829 kb on + strand at 3966.830 kb on - strand at 3966.883 kb on + strand at 3966.883 kb on + strand at 3966.883 kb on + strand at 3966.891 kb on + strand at 3966.891 kb on + strand at 3966.891 kb on + strand at 3966.891 kb on + strand at 3966.891 kb on + strand at 3966.891 kb on + strand at 3966.891 kb on + strand at 3966.891 kb on + strand at 3966.891 kb on + strand at 3966.892 kb on - strand at 3966.892 kb on - strand at 3966.892 kb on - strand at 3966.892 kb on - strand at 3966.892 kb on - strand at 3966.892 kb on - strand at 3966.892 kb on - strand at 3966.892 kb on - strand at 3966.892 kb on - strand at 3966.923 kb on + strand at 3966.937 kb on + strand at 3966.938 kb on - strand at 3967.081 kb on - strand at 3967.081 kb on - strand at 3967.081 kb on - strand at 3967.081 kb on - strand at 3967.081 kb on - strand at 3967.081 kb on - strand at 3967.081 kb on - strand at 3967.168 kb on + strand at 3967.169 kb on - strand at 3967.169 kb on - strand at 3967.169 kb on - strand at 3967.169 kb on - strand at 3967.169 kb on - strand at 3967.169 kb on - strand at 3967.170 kb on + strand at 3967.170 kb on + strand at 3967.170 kb on + strand at 3967.170 kb on + strand at 3967.170 kb on + strand at 3967.170 kb on + strand at 3967.171 kb on - strand at 3967.171 kb on - strand at 3967.171 kb on - strand at 3967.171 kb on - strand at 3967.171 kb on - strand at 3967.171 kb on - strand at 3967.171 kb on - strand at 3967.171 kb on - strand at 3967.751 kb on + strand, within Pf1N1B4_3564 at 3967.752 kb on - strand, within Pf1N1B4_3564 at 3967.752 kb on - strand, within Pf1N1B4_3564 at 3967.752 kb on - strand, within Pf1N1B4_3564 at 3967.752 kb on - strand, within Pf1N1B4_3564 at 3967.752 kb on - strand, within Pf1N1B4_3564 at 3967.752 kb on - strand, within Pf1N1B4_3564 at 3967.752 kb on - strand, within Pf1N1B4_3564 at 3967.824 kb on + strand, within Pf1N1B4_3564 at 3967.825 kb on - strand, within Pf1N1B4_3564 at 3968.076 kb on + strand, within Pf1N1B4_3564 at 3968.076 kb on + strand, within Pf1N1B4_3564 at 3968.077 kb on - strand, within Pf1N1B4_3564 at 3968.077 kb on - strand, within Pf1N1B4_3564 at 3968.163 kb on + strand at 3968.164 kb on - strand at 3968.164 kb on - strand at 3968.164 kb on - strand at 3968.164 kb on - strand at 3968.164 kb on - strand at 3968.164 kb on - strand at 3968.212 kb on - strand at 3968.273 kb on + strand at 3968.273 kb on + strand at 3968.273 kb on + strand at 3968.273 kb on + strand at 3968.274 kb on - strand at 3968.274 kb on - strand at 3968.682 kb on + strand at 3968.781 kb on + strand at 3968.781 kb on + strand at 3968.782 kb on - strand at 3968.782 kb on - strand at 3968.782 kb on - strand at 3968.782 kb on - strand at 3968.855 kb on + strand, within Pf1N1B4_3566 at 3968.855 kb on - strand at 3968.856 kb on - strand, within Pf1N1B4_3566 at 3968.856 kb on - strand, within Pf1N1B4_3566 at 3968.856 kb on - strand, within Pf1N1B4_3566 at 3968.856 kb on - strand at 3968.856 kb on - strand, within Pf1N1B4_3566 at 3968.856 kb on - strand, within Pf1N1B4_3566 at 3968.856 kb on - strand, within Pf1N1B4_3566 at 3968.984 kb on + strand, within Pf1N1B4_3566 at 3968.985 kb on - strand, within Pf1N1B4_3566 at 3969.038 kb on + strand, within Pf1N1B4_3566 at 3969.039 kb on - strand, within Pf1N1B4_3566 at 3969.054 kb on - strand, within Pf1N1B4_3566 at 3969.054 kb on - strand, within Pf1N1B4_3566 at 3969.080 kb on - strand, within Pf1N1B4_3566 at 3969.096 kb on - strand, within Pf1N1B4_3566 at 3969.096 kb on - strand, within Pf1N1B4_3566 at 3969.096 kb on - strand, within Pf1N1B4_3566 at 3969.183 kb on - strand, within Pf1N1B4_3566
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 3,966,128 + Pf1N1B4_3561 0.86 -0.1 3,966,128 + Pf1N1B4_3561 0.86 +0.2 3,966,188 + -0.3 3,966,188 + -0.1 3,966,188 + -1.7 3,966,199 + -1.9 3,966,199 + +0.9 3,966,199 + +0.6 3,966,200 - -1.1 3,966,200 - -2.1 3,966,327 + +1.9 3,966,328 - +0.9 3,966,331 + +0.9 3,966,331 + +1.5 3,966,331 + -1.1 3,966,332 - -1.4 3,966,332 - -0.1 3,966,332 - +0.9 3,966,332 - +0.7 3,966,332 - -1.1 3,966,332 - -1.1 3,966,332 - -0.1 3,966,340 + +0.9 3,966,340 + -0.1 3,966,340 + +1.1 3,966,341 - -1.1 3,966,341 - +1.9 3,966,341 - -1.7 3,966,384 + +1.5 3,966,385 - -0.1 3,966,385 - +0.5 3,966,624 + +0.5 3,966,624 + -0.1 3,966,624 + +0.1 3,966,624 + +2.2 3,966,624 + +0.2 3,966,624 + +0.7 3,966,624 + +1.5 3,966,624 + +0.3 3,966,627 + -1.1 3,966,660 + +0.5 3,966,778 + Pf1N1B4_3563 0.88 -0.2 3,966,809 - +0.6 3,966,820 + -1.1 3,966,820 + -1.1 3,966,821 - +0.9 3,966,821 - +0.3 3,966,829 + -1.1 3,966,830 - +0.5 3,966,883 + -0.1 3,966,883 + -1.1 3,966,883 + +0.7 3,966,891 + +1.7 3,966,891 + -0.7 3,966,891 + -0.1 3,966,891 + -1.0 3,966,891 + -0.6 3,966,891 + -1.9 3,966,891 + -0.1 3,966,891 + +1.5 3,966,891 + +0.1 3,966,892 - -0.1 3,966,892 - -0.1 3,966,892 - -0.4 3,966,892 - +0.6 3,966,892 - -0.1 3,966,892 - -0.6 3,966,892 - +1.9 3,966,892 - +0.1 3,966,892 - +1.5 3,966,923 + -0.9 3,966,937 + +0.5 3,966,938 - -0.6 3,967,081 - +2.2 3,967,081 - -1.1 3,967,081 - -0.5 3,967,081 - +0.9 3,967,081 - -1.7 3,967,081 - -1.7 3,967,081 - -0.9 3,967,168 + -0.1 3,967,169 - -0.4 3,967,169 - -1.1 3,967,169 - -2.1 3,967,169 - -0.1 3,967,169 - -0.1 3,967,169 - +0.6 3,967,170 + +1.9 3,967,170 + +1.5 3,967,170 + +1.5 3,967,170 + +0.9 3,967,170 + -2.4 3,967,170 + -0.1 3,967,171 - +0.6 3,967,171 - +1.2 3,967,171 - -1.3 3,967,171 - -1.1 3,967,171 - -0.1 3,967,171 - -0.4 3,967,171 - -0.1 3,967,171 - -1.1 3,967,751 + Pf1N1B4_3564 0.56 -2.7 3,967,752 - Pf1N1B4_3564 0.56 -0.7 3,967,752 - Pf1N1B4_3564 0.56 +0.3 3,967,752 - Pf1N1B4_3564 0.56 -1.1 3,967,752 - Pf1N1B4_3564 0.56 -1.7 3,967,752 - Pf1N1B4_3564 0.56 -0.3 3,967,752 - Pf1N1B4_3564 0.56 +1.6 3,967,752 - Pf1N1B4_3564 0.56 +1.9 3,967,824 + Pf1N1B4_3564 0.62 -1.7 3,967,825 - Pf1N1B4_3564 0.62 +0.2 3,968,076 + Pf1N1B4_3564 0.84 +0.5 3,968,076 + Pf1N1B4_3564 0.84 -0.5 3,968,077 - Pf1N1B4_3564 0.84 +1.5 3,968,077 - Pf1N1B4_3564 0.84 +0.6 3,968,163 + -0.1 3,968,164 - -0.0 3,968,164 - +0.4 3,968,164 - +2.7 3,968,164 - -0.5 3,968,164 - +0.3 3,968,164 - -1.1 3,968,212 - +1.2 3,968,273 + +0.9 3,968,273 + -0.5 3,968,273 + -1.4 3,968,273 + -0.1 3,968,274 - -0.7 3,968,274 - +1.1 3,968,682 + -1.7 3,968,781 + -0.1 3,968,781 + +0.9 3,968,782 - +0.1 3,968,782 - -1.3 3,968,782 - -1.1 3,968,782 - -0.4 3,968,855 + Pf1N1B4_3566 0.15 -0.1 3,968,855 - -1.1 3,968,856 - Pf1N1B4_3566 0.16 -1.9 3,968,856 - Pf1N1B4_3566 0.16 -0.1 3,968,856 - Pf1N1B4_3566 0.16 -0.3 3,968,856 - -0.1 3,968,856 - Pf1N1B4_3566 0.16 +0.9 3,968,856 - Pf1N1B4_3566 0.16 -2.4 3,968,856 - Pf1N1B4_3566 0.16 -1.3 3,968,984 + Pf1N1B4_3566 0.32 -0.6 3,968,985 - Pf1N1B4_3566 0.32 -0.1 3,969,038 + Pf1N1B4_3566 0.39 -1.1 3,969,039 - Pf1N1B4_3566 0.39 -0.5 3,969,054 - Pf1N1B4_3566 0.41 +1.2 3,969,054 - Pf1N1B4_3566 0.41 -0.9 3,969,080 - Pf1N1B4_3566 0.44 -1.1 3,969,096 - Pf1N1B4_3566 0.46 -0.1 3,969,096 - Pf1N1B4_3566 0.46 +0.0 3,969,096 - Pf1N1B4_3566 0.46 -0.3 3,969,183 - Pf1N1B4_3566 0.57 +0.6
Or see this region's nucleotide sequence