Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3449 and Pf1N1B4_3450 are separated by 146 nucleotides Pf1N1B4_3450 and Pf1N1B4_3451 are separated by 90 nucleotides
Pf1N1B4_3449 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 3,844,674 to 3,845,678
_3449
Pf1N1B4_3450 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 3,845,825 to 3,848,446
_3450
Pf1N1B4_3451 - Aspartokinase (EC 2.7.2.4), at 3,848,537 to 3,849,778
_3451
Position (kb)
3845
3846
3847
3848
3849 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3845.122 kb on - strand, within Pf1N1B4_3449 at 3845.122 kb on - strand, within Pf1N1B4_3449 at 3845.190 kb on + strand, within Pf1N1B4_3449 at 3845.190 kb on + strand, within Pf1N1B4_3449 at 3845.191 kb on - strand, within Pf1N1B4_3449 at 3845.191 kb on - strand, within Pf1N1B4_3449 at 3845.397 kb on + strand, within Pf1N1B4_3449 at 3845.398 kb on - strand, within Pf1N1B4_3449 at 3845.398 kb on - strand, within Pf1N1B4_3449 at 3845.514 kb on + strand, within Pf1N1B4_3449 at 3845.514 kb on + strand, within Pf1N1B4_3449 at 3845.514 kb on + strand, within Pf1N1B4_3449 at 3845.514 kb on + strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.707 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.729 kb on + strand at 3845.729 kb on + strand at 3845.729 kb on + strand at 3845.729 kb on + strand at 3845.729 kb on + strand at 3845.745 kb on + strand at 3845.745 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3848.430 kb on + strand at 3848.430 kb on + strand at 3848.430 kb on + strand at 3848.430 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.501 kb on + strand at 3848.501 kb on + strand at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 3,844,905 + Pf1N1B4_3449 0.23 -1.7 3,844,905 + Pf1N1B4_3449 0.23 +0.3 3,844,905 + Pf1N1B4_3449 0.23 -1.7 3,844,905 + Pf1N1B4_3449 0.23 -0.8 3,844,905 + Pf1N1B4_3449 0.23 +0.9 3,844,906 - Pf1N1B4_3449 0.23 -1.7 3,844,906 - Pf1N1B4_3449 0.23 -0.3 3,844,906 - Pf1N1B4_3449 0.23 -2.4 3,844,906 - Pf1N1B4_3449 0.23 -0.7 3,844,906 - Pf1N1B4_3449 0.23 +0.0 3,844,906 - Pf1N1B4_3449 0.23 +0.2 3,844,906 - Pf1N1B4_3449 0.23 +0.1 3,844,932 + Pf1N1B4_3449 0.26 +0.5 3,844,932 + Pf1N1B4_3449 0.26 -0.1 3,844,932 + +0.3 3,844,932 + Pf1N1B4_3449 0.26 -0.8 3,844,932 + Pf1N1B4_3449 0.26 +1.1 3,844,932 + Pf1N1B4_3449 0.26 -2.4 3,844,932 + Pf1N1B4_3449 0.26 -0.1 3,844,933 - Pf1N1B4_3449 0.26 -0.7 3,844,933 - Pf1N1B4_3449 0.26 +1.3 3,844,933 - +0.9 3,844,933 - Pf1N1B4_3449 0.26 -0.5 3,844,933 - Pf1N1B4_3449 0.26 -1.1 3,844,933 - Pf1N1B4_3449 0.26 -1.1 3,844,933 - Pf1N1B4_3449 0.26 -0.7 3,844,933 - Pf1N1B4_3449 0.26 -1.1 3,844,933 - Pf1N1B4_3449 0.26 -0.1 3,844,933 - Pf1N1B4_3449 0.26 -1.7 3,844,933 - Pf1N1B4_3449 0.26 -0.1 3,844,933 - Pf1N1B4_3449 0.26 +0.5 3,844,934 + Pf1N1B4_3449 0.26 -1.1 3,844,934 + Pf1N1B4_3449 0.26 -0.1 3,844,934 + Pf1N1B4_3449 0.26 -1.1 3,844,934 + Pf1N1B4_3449 0.26 +0.9 3,844,934 + Pf1N1B4_3449 0.26 -1.7 3,844,934 + Pf1N1B4_3449 0.26 +0.7 3,844,934 + Pf1N1B4_3449 0.26 +0.8 3,844,935 - Pf1N1B4_3449 0.26 -2.1 3,844,935 - Pf1N1B4_3449 0.26 +1.5 3,844,935 - Pf1N1B4_3449 0.26 -0.7 3,844,935 - Pf1N1B4_3449 0.26 +0.1 3,844,935 - Pf1N1B4_3449 0.26 -0.7 3,844,935 - Pf1N1B4_3449 0.26 -0.3 3,844,935 - Pf1N1B4_3449 0.26 -0.7 3,844,939 - -0.1 3,844,939 - Pf1N1B4_3449 0.26 +0.3 3,844,939 - Pf1N1B4_3449 0.26 -0.7 3,844,939 - Pf1N1B4_3449 0.26 +2.1 3,844,939 - Pf1N1B4_3449 0.26 -0.5 3,844,939 - Pf1N1B4_3449 0.26 +0.5 3,844,939 - Pf1N1B4_3449 0.26 -0.7 3,844,939 - Pf1N1B4_3449 0.26 -1.1 3,845,122 - Pf1N1B4_3449 0.45 -0.4 3,845,122 - Pf1N1B4_3449 0.45 -1.7 3,845,190 + Pf1N1B4_3449 0.51 -0.5 3,845,190 + Pf1N1B4_3449 0.51 +1.9 3,845,191 - Pf1N1B4_3449 0.51 +0.3 3,845,191 - Pf1N1B4_3449 0.51 +0.3 3,845,397 + Pf1N1B4_3449 0.72 +0.3 3,845,398 - Pf1N1B4_3449 0.72 -0.3 3,845,398 - Pf1N1B4_3449 0.72 +0.9 3,845,514 + Pf1N1B4_3449 0.84 +0.3 3,845,514 + Pf1N1B4_3449 0.84 -0.1 3,845,514 + Pf1N1B4_3449 0.84 +0.5 3,845,514 + Pf1N1B4_3449 0.84 -1.1 3,845,515 - Pf1N1B4_3449 0.84 -0.7 3,845,515 - Pf1N1B4_3449 0.84 -0.1 3,845,515 - Pf1N1B4_3449 0.84 -0.1 3,845,515 - Pf1N1B4_3449 0.84 -0.1 3,845,515 - Pf1N1B4_3449 0.84 +1.5 3,845,515 - +1.9 3,845,515 - Pf1N1B4_3449 0.84 -0.5 3,845,707 + +0.1 3,845,725 + -0.1 3,845,725 + -0.1 3,845,725 + -0.7 3,845,725 + +0.7 3,845,725 + -0.7 3,845,725 + +1.2 3,845,729 + +0.5 3,845,729 + -0.8 3,845,729 + -1.1 3,845,729 + +0.1 3,845,729 + -0.8 3,845,745 + -2.9 3,845,745 + -2.2 3,845,747 + -0.5 3,845,747 + -0.2 3,845,747 + -0.7 3,845,747 + -0.7 3,845,747 + -0.1 3,845,747 + -0.1 3,845,747 + +0.6 3,848,430 + -0.3 3,848,430 + +0.5 3,848,430 + +0.3 3,848,430 + +1.9 3,848,442 + +0.2 3,848,442 + +1.9 3,848,442 + -0.3 3,848,442 + +0.9 3,848,442 + -0.4 3,848,442 + -0.5 3,848,442 + +0.7 3,848,442 + -0.1 3,848,442 + -1.4 3,848,444 + -0.1 3,848,444 + +0.3 3,848,444 + +0.2 3,848,444 + -0.1 3,848,444 + -1.1 3,848,444 + -0.1 3,848,501 + +0.4 3,848,501 + -0.6 3,849,262 + Pf1N1B4_3451 0.58 -0.1 3,849,262 + Pf1N1B4_3451 0.58 -0.5 3,849,262 + Pf1N1B4_3451 0.58 +0.9 3,849,262 + Pf1N1B4_3451 0.58 -1.4 3,849,262 + Pf1N1B4_3451 0.58 -1.4 3,849,265 + Pf1N1B4_3451 0.59 +0.2 3,849,265 + Pf1N1B4_3451 0.59 -0.9 3,849,265 + Pf1N1B4_3451 0.59 +0.9 3,849,265 + Pf1N1B4_3451 0.59 -0.1 3,849,265 + Pf1N1B4_3451 0.59 +0.5 3,849,265 + Pf1N1B4_3451 0.59 +1.5
Or see this region's nucleotide sequence