Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2822 and Pf1N1B4_2823 overlap by 4 nucleotides Pf1N1B4_2823 and Pf1N1B4_2824 are separated by 129 nucleotides Pf1N1B4_2824 and Pf1N1B4_5202 are separated by 300 nucleotides Pf1N1B4_5202 and Pf1N1B4_5203 are separated by 12 nucleotides Pf1N1B4_5203 and Pf1N1B4_2825 are separated by 109 nucleotides
Pf1N1B4_2822 - Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70), at 3,165,270 to 3,166,190
_2822
Pf1N1B4_2823 - COG1496: Uncharacterized conserved protein, at 3,166,187 to 3,166,912
_2823
Pf1N1B4_2824 - ClpB protein, at 3,167,042 to 3,169,606
_2824
Pf1N1B4_5202 - tRNA-Asn-GTT, at 3,169,907 to 3,169,979
_5202
Pf1N1B4_5203 - tRNA-Pro-TGG, at 3,169,992 to 3,170,065
_5203
Pf1N1B4_2825 - EF hand domain protein, at 3,170,175 to 3,170,576
_2825
Position (kb)
3167
3168
3169
3170 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3166.363 kb on + strand at 3166.763 kb on + strand, within Pf1N1B4_2823 at 3166.763 kb on + strand, within Pf1N1B4_2823 at 3166.763 kb on + strand, within Pf1N1B4_2823 at 3166.780 kb on - strand, within Pf1N1B4_2823 at 3166.812 kb on - strand, within Pf1N1B4_2823 at 3166.910 kb on + strand at 3166.910 kb on + strand at 3166.970 kb on + strand at 3166.970 kb on + strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3167.008 kb on + strand at 3167.008 kb on + strand at 3167.009 kb on - strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.047 kb on + strand at 3167.047 kb on + strand at 3167.048 kb on - strand at 3167.048 kb on - strand at 3167.048 kb on - strand at 3167.049 kb on + strand at 3167.049 kb on + strand at 3167.049 kb on + strand at 3167.049 kb on + strand at 3167.050 kb on - strand at 3167.076 kb on + strand at 3167.224 kb on + strand at 3167.244 kb on + strand at 3167.245 kb on - strand at 3167.302 kb on - strand, within Pf1N1B4_2824 at 3167.302 kb on - strand, within Pf1N1B4_2824 at 3167.302 kb on - strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3168.005 kb on + strand, within Pf1N1B4_2824 at 3168.005 kb on + strand, within Pf1N1B4_2824 at 3168.005 kb on + strand, within Pf1N1B4_2824 at 3168.006 kb on - strand, within Pf1N1B4_2824 at 3168.006 kb on - strand, within Pf1N1B4_2824 at 3168.007 kb on + strand, within Pf1N1B4_2824 at 3168.007 kb on + strand, within Pf1N1B4_2824 at 3168.007 kb on + strand, within Pf1N1B4_2824 at 3168.008 kb on - strand, within Pf1N1B4_2824 at 3168.009 kb on + strand, within Pf1N1B4_2824 at 3168.009 kb on + strand, within Pf1N1B4_2824 at 3168.009 kb on + strand, within Pf1N1B4_2824 at 3168.009 kb on + strand, within Pf1N1B4_2824 at 3168.009 kb on + strand, within Pf1N1B4_2824 at 3168.010 kb on - strand at 3168.010 kb on - strand, within Pf1N1B4_2824 at 3168.010 kb on - strand, within Pf1N1B4_2824 at 3168.010 kb on - strand, within Pf1N1B4_2824 at 3168.010 kb on - strand, within Pf1N1B4_2824 at 3168.179 kb on + strand, within Pf1N1B4_2824 at 3168.179 kb on + strand, within Pf1N1B4_2824 at 3168.179 kb on + strand, within Pf1N1B4_2824 at 3168.179 kb on + strand, within Pf1N1B4_2824 at 3168.179 kb on + strand, within Pf1N1B4_2824 at 3168.179 kb on + strand, within Pf1N1B4_2824 at 3168.179 kb on + strand, within Pf1N1B4_2824 at 3168.180 kb on - strand, within Pf1N1B4_2824 at 3168.180 kb on - strand, within Pf1N1B4_2824 at 3168.405 kb on - strand, within Pf1N1B4_2824 at 3168.998 kb on + strand, within Pf1N1B4_2824 at 3169.175 kb on - strand, within Pf1N1B4_2824 at 3169.438 kb on + strand at 3169.457 kb on + strand at 3169.547 kb on + strand at 3169.547 kb on + strand at 3169.691 kb on + strand at 3169.753 kb on + strand at 3169.753 kb on + strand at 3170.069 kb on + strand at 3170.072 kb on + strand at 3170.072 kb on + strand at 3170.072 kb on + strand at 3170.176 kb on + strand at 3170.176 kb on + strand at 3170.176 kb on + strand at 3170.176 kb on + strand at 3170.177 kb on - strand at 3170.177 kb on - strand at 3170.177 kb on - strand at 3170.177 kb on - strand at 3170.180 kb on + strand at 3170.180 kb on + strand at 3170.180 kb on + strand at 3170.180 kb on + strand at 3170.181 kb on - strand at 3170.181 kb on - strand at 3170.181 kb on - strand at 3170.181 kb on - strand at 3170.181 kb on - strand at 3170.236 kb on + strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.418 kb on - strand, within Pf1N1B4_2825 at 3170.418 kb on - strand, within Pf1N1B4_2825 at 3170.418 kb on - strand, within Pf1N1B4_2825 at 3170.461 kb on + strand, within Pf1N1B4_2825 at 3170.462 kb on - strand, within Pf1N1B4_2825 at 3170.462 kb on - strand, within Pf1N1B4_2825 at 3170.562 kb on - strand at 3170.577 kb on + strand at 3170.578 kb on - strand at 3170.578 kb on - strand at 3170.578 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 3,166,363 + -0.5 3,166,763 + Pf1N1B4_2823 0.79 -1.7 3,166,763 + Pf1N1B4_2823 0.79 +0.4 3,166,763 + Pf1N1B4_2823 0.79 -1.2 3,166,780 - Pf1N1B4_2823 0.82 +0.3 3,166,812 - Pf1N1B4_2823 0.86 +0.1 3,166,910 + -0.9 3,166,910 + +2.1 3,166,970 + +0.1 3,166,970 + -0.4 3,166,971 - -0.9 3,166,971 - -0.9 3,166,971 - -1.9 3,166,971 - +0.3 3,166,971 - -2.2 3,166,971 - +0.1 3,166,971 - -0.3 3,166,971 - -1.5 3,167,008 + -1.9 3,167,008 + +0.1 3,167,009 - -0.3 3,167,010 + +0.4 3,167,010 + -0.1 3,167,010 + -0.5 3,167,010 + +0.3 3,167,010 + -0.5 3,167,010 + +0.3 3,167,010 + +0.1 3,167,011 - -1.0 3,167,011 - -0.2 3,167,011 - +0.5 3,167,011 - +0.5 3,167,011 - +0.1 3,167,011 - +0.1 3,167,047 + -0.4 3,167,047 + +1.7 3,167,048 - -1.2 3,167,048 - -0.9 3,167,048 - -0.5 3,167,049 + -1.9 3,167,049 + -0.6 3,167,049 + -1.5 3,167,049 + -0.5 3,167,050 - -0.3 3,167,076 + -1.5 3,167,224 + +1.1 3,167,244 + +1.7 3,167,245 - -0.5 3,167,302 - Pf1N1B4_2824 0.10 -2.0 3,167,302 - Pf1N1B4_2824 0.10 -1.0 3,167,302 - Pf1N1B4_2824 0.10 +0.1 3,167,531 + Pf1N1B4_2824 0.19 +0.5 3,167,531 + Pf1N1B4_2824 0.19 -1.9 3,167,531 + Pf1N1B4_2824 0.19 +1.4 3,167,531 + Pf1N1B4_2824 0.19 +0.5 3,167,531 + Pf1N1B4_2824 0.19 -0.7 3,167,531 + Pf1N1B4_2824 0.19 +0.5 3,167,532 - Pf1N1B4_2824 0.19 +1.1 3,167,532 - Pf1N1B4_2824 0.19 -0.9 3,167,532 - Pf1N1B4_2824 0.19 +0.1 3,167,532 - Pf1N1B4_2824 0.19 -0.5 3,167,532 - Pf1N1B4_2824 0.19 -1.3 3,167,532 - Pf1N1B4_2824 0.19 +0.4 3,168,005 + Pf1N1B4_2824 0.38 +0.1 3,168,005 + Pf1N1B4_2824 0.38 +0.1 3,168,005 + Pf1N1B4_2824 0.38 -0.7 3,168,006 - Pf1N1B4_2824 0.38 -1.2 3,168,006 - Pf1N1B4_2824 0.38 +0.1 3,168,007 + Pf1N1B4_2824 0.38 -0.5 3,168,007 + Pf1N1B4_2824 0.38 +0.7 3,168,007 + Pf1N1B4_2824 0.38 -0.9 3,168,008 - Pf1N1B4_2824 0.38 +0.1 3,168,009 + Pf1N1B4_2824 0.38 +0.5 3,168,009 + Pf1N1B4_2824 0.38 -0.9 3,168,009 + Pf1N1B4_2824 0.38 +0.1 3,168,009 + Pf1N1B4_2824 0.38 +1.3 3,168,009 + Pf1N1B4_2824 0.38 +1.1 3,168,010 - +0.7 3,168,010 - Pf1N1B4_2824 0.38 +1.1 3,168,010 - Pf1N1B4_2824 0.38 -0.1 3,168,010 - Pf1N1B4_2824 0.38 -1.1 3,168,010 - Pf1N1B4_2824 0.38 -1.9 3,168,179 + Pf1N1B4_2824 0.44 +0.1 3,168,179 + Pf1N1B4_2824 0.44 -0.4 3,168,179 + Pf1N1B4_2824 0.44 -0.9 3,168,179 + Pf1N1B4_2824 0.44 -0.5 3,168,179 + Pf1N1B4_2824 0.44 +0.8 3,168,179 + Pf1N1B4_2824 0.44 -0.4 3,168,179 + Pf1N1B4_2824 0.44 -0.6 3,168,180 - Pf1N1B4_2824 0.44 -1.9 3,168,180 - Pf1N1B4_2824 0.44 -0.3 3,168,405 - Pf1N1B4_2824 0.53 +1.1 3,168,998 + Pf1N1B4_2824 0.76 -2.7 3,169,175 - Pf1N1B4_2824 0.83 +0.8 3,169,438 + -0.9 3,169,457 + -0.9 3,169,547 + +0.4 3,169,547 + -0.2 3,169,691 + -0.2 3,169,753 + +0.1 3,169,753 + -1.2 3,170,069 + -0.9 3,170,072 + -0.9 3,170,072 + +0.8 3,170,072 + -0.9 3,170,176 + -0.2 3,170,176 + -1.7 3,170,176 + +0.1 3,170,176 + +0.1 3,170,177 - -0.3 3,170,177 - -0.3 3,170,177 - +0.7 3,170,177 - -0.9 3,170,180 + +0.1 3,170,180 + -0.5 3,170,180 + -0.7 3,170,180 + -1.5 3,170,181 - +0.1 3,170,181 - -1.1 3,170,181 - +0.1 3,170,181 - -0.1 3,170,181 - +1.9 3,170,236 + Pf1N1B4_2825 0.15 -0.9 3,170,237 - Pf1N1B4_2825 0.15 -0.9 3,170,237 - Pf1N1B4_2825 0.15 -0.9 3,170,237 - Pf1N1B4_2825 0.15 -1.5 3,170,237 - Pf1N1B4_2825 0.15 -1.9 3,170,237 - Pf1N1B4_2825 0.15 -1.9 3,170,237 - Pf1N1B4_2825 0.15 -0.2 3,170,237 - Pf1N1B4_2825 0.15 +1.7 3,170,418 - Pf1N1B4_2825 0.60 -0.4 3,170,418 - Pf1N1B4_2825 0.60 +0.1 3,170,418 - Pf1N1B4_2825 0.60 +0.1 3,170,461 + Pf1N1B4_2825 0.71 +0.5 3,170,462 - Pf1N1B4_2825 0.71 -0.9 3,170,462 - Pf1N1B4_2825 0.71 -1.7 3,170,562 - -0.9 3,170,577 + -1.0 3,170,578 - -0.6 3,170,578 - +0.1 3,170,578 - -1.5
Or see this region's nucleotide sequence