Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2799 and Pf1N1B4_2800 are separated by 123 nucleotides Pf1N1B4_2800 and Pf1N1B4_2801 are separated by 12 nucleotides Pf1N1B4_2801 and Pf1N1B4_2802 are separated by 184 nucleotides
Pf1N1B4_2799 - GTP-binding protein Obg, at 3,141,980 to 3,143,203
_2799
Pf1N1B4_2800 - Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA, at 3,143,327 to 3,144,445
_2800
Pf1N1B4_2801 - Conserved uncharacterized protein CreA, at 3,144,458 to 3,144,922
_2801
Pf1N1B4_2802 - SSU ribosomal protein S20p, at 3,145,107 to 3,145,385
_2802
Position (kb)
3143
3144
3145 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3143.189 kb on + strand at 3143.189 kb on + strand at 3143.189 kb on + strand at 3143.189 kb on + strand at 3143.189 kb on + strand at 3143.220 kb on + strand at 3143.220 kb on + strand at 3143.220 kb on + strand at 3143.234 kb on + strand at 3143.247 kb on + strand at 3143.247 kb on + strand at 3143.247 kb on + strand at 3143.247 kb on + strand at 3143.247 kb on + strand at 3143.247 kb on + strand at 3143.247 kb on + strand at 3143.250 kb on + strand at 3143.250 kb on + strand at 3144.451 kb on + strand at 3144.477 kb on + strand at 3144.620 kb on + strand, within Pf1N1B4_2801 at 3144.620 kb on + strand, within Pf1N1B4_2801 at 3144.620 kb on + strand, within Pf1N1B4_2801 at 3144.620 kb on + strand, within Pf1N1B4_2801 at 3144.621 kb on - strand, within Pf1N1B4_2801 at 3144.648 kb on + strand, within Pf1N1B4_2801 at 3144.660 kb on + strand, within Pf1N1B4_2801 at 3144.660 kb on + strand, within Pf1N1B4_2801 at 3144.661 kb on - strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.691 kb on + strand, within Pf1N1B4_2801 at 3144.823 kb on + strand, within Pf1N1B4_2801 at 3144.823 kb on + strand, within Pf1N1B4_2801 at 3144.824 kb on - strand, within Pf1N1B4_2801 at 3144.833 kb on + strand, within Pf1N1B4_2801 at 3144.833 kb on + strand, within Pf1N1B4_2801 at 3144.833 kb on + strand, within Pf1N1B4_2801 at 3144.833 kb on + strand, within Pf1N1B4_2801 at 3144.834 kb on - strand, within Pf1N1B4_2801 at 3144.834 kb on - strand, within Pf1N1B4_2801 at 3144.842 kb on + strand, within Pf1N1B4_2801 at 3144.842 kb on + strand, within Pf1N1B4_2801 at 3144.842 kb on + strand, within Pf1N1B4_2801 at 3144.925 kb on + strand at 3144.925 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.985 kb on + strand at 3144.986 kb on - strand at 3144.986 kb on - strand at 3144.986 kb on - strand at 3145.015 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 3,143,189 + +0.7 3,143,189 + -1.2 3,143,189 + -0.9 3,143,189 + -0.5 3,143,189 + -0.5 3,143,220 + -0.5 3,143,220 + -0.9 3,143,220 + -0.5 3,143,234 + +0.1 3,143,247 + -1.5 3,143,247 + +1.7 3,143,247 + -0.2 3,143,247 + +0.4 3,143,247 + +0.6 3,143,247 + +1.1 3,143,247 + +1.7 3,143,250 + -0.5 3,143,250 + -0.4 3,144,451 + -1.5 3,144,477 + -1.9 3,144,620 + Pf1N1B4_2801 0.35 +0.1 3,144,620 + Pf1N1B4_2801 0.35 +1.1 3,144,620 + Pf1N1B4_2801 0.35 -0.5 3,144,620 + Pf1N1B4_2801 0.35 +0.5 3,144,621 - Pf1N1B4_2801 0.35 -0.7 3,144,648 + Pf1N1B4_2801 0.41 +0.7 3,144,660 + Pf1N1B4_2801 0.43 -0.5 3,144,660 + Pf1N1B4_2801 0.43 +1.1 3,144,661 - Pf1N1B4_2801 0.44 +1.7 3,144,691 + Pf1N1B4_2801 0.50 +0.1 3,144,691 + Pf1N1B4_2801 0.50 -1.5 3,144,691 + Pf1N1B4_2801 0.50 -1.0 3,144,691 + Pf1N1B4_2801 0.50 +0.7 3,144,691 + Pf1N1B4_2801 0.50 +0.5 3,144,691 + Pf1N1B4_2801 0.50 +0.1 3,144,691 + Pf1N1B4_2801 0.50 -1.9 3,144,691 + Pf1N1B4_2801 0.50 +0.4 3,144,691 + Pf1N1B4_2801 0.50 -0.5 3,144,691 + Pf1N1B4_2801 0.50 +0.1 3,144,823 + Pf1N1B4_2801 0.78 +0.1 3,144,823 + Pf1N1B4_2801 0.78 -0.5 3,144,824 - Pf1N1B4_2801 0.79 -1.7 3,144,833 + Pf1N1B4_2801 0.81 -0.4 3,144,833 + Pf1N1B4_2801 0.81 -0.2 3,144,833 + Pf1N1B4_2801 0.81 +1.1 3,144,833 + Pf1N1B4_2801 0.81 -0.5 3,144,834 - Pf1N1B4_2801 0.81 +0.4 3,144,834 - Pf1N1B4_2801 0.81 +0.1 3,144,842 + Pf1N1B4_2801 0.83 +2.1 3,144,842 + Pf1N1B4_2801 0.83 +0.8 3,144,842 + Pf1N1B4_2801 0.83 +0.2 3,144,925 + -1.2 3,144,925 + +0.5 3,144,985 + +0.1 3,144,985 + -0.9 3,144,985 + -0.5 3,144,985 + -1.7 3,144,985 + +0.1 3,144,985 + +0.1 3,144,985 + -0.7 3,144,985 + +1.4 3,144,985 + -0.1 3,144,985 + -0.9 3,144,985 + -0.0 3,144,985 + -0.9 3,144,985 + +1.1 3,144,985 + -2.2 3,144,985 + +0.1 3,144,985 + -1.9 3,144,985 + -0.9 3,144,985 + +0.5 3,144,985 + -0.2 3,144,985 + -2.2 3,144,985 + -1.1 3,144,985 + -0.1 3,144,986 - -1.5 3,144,986 - -1.5 3,144,986 - +1.1 3,145,015 - -0.6
Or see this region's nucleotide sequence