Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2661 and Pf1N1B4_2662 are separated by 91 nucleotides Pf1N1B4_2662 and Pf1N1B4_2663 overlap by 4 nucleotides Pf1N1B4_2663 and Pf1N1B4_2664 are separated by 273 nucleotides
Pf1N1B4_2661 - 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7), at 2,997,228 to 2,999,126
_2661
Pf1N1B4_2662 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29), at 2,999,218 to 3,000,105
_2662
Pf1N1B4_2663 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 3,000,102 to 3,000,344
_2663
Pf1N1B4_2664 - Fic family protein, at 3,000,618 to 3,001,763
_2664
Position (kb)
2999
3000
3001 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2999.140 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.142 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.146 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 2999.148 kb on - strand at 3000.368 kb on - strand at 3000.409 kb on + strand at 3000.409 kb on + strand at 3000.409 kb on + strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.410 kb on - strand at 3000.420 kb on + strand at 3000.619 kb on + strand at 3000.620 kb on - strand at 3000.761 kb on - strand, within Pf1N1B4_2664 at 3000.822 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.923 kb on + strand, within Pf1N1B4_2664 at 3000.974 kb on + strand at 3000.974 kb on + strand, within Pf1N1B4_2664 at 3000.975 kb on - strand at 3001.046 kb on - strand, within Pf1N1B4_2664 at 3001.046 kb on - strand at 3001.083 kb on + strand, within Pf1N1B4_2664
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,999,140 - +0.2 2,999,142 - +0.1 2,999,142 - -0.3 2,999,142 - -1.2 2,999,142 - +0.1 2,999,142 - +0.1 2,999,142 - +0.5 2,999,142 - +1.1 2,999,142 - -0.4 2,999,142 - -1.3 2,999,142 - +0.7 2,999,142 - -0.2 2,999,142 - -1.4 2,999,142 - -0.9 2,999,142 - +0.7 2,999,142 - -0.6 2,999,142 - +0.1 2,999,142 - +1.4 2,999,142 - -0.6 2,999,142 - -1.2 2,999,142 - -0.1 2,999,142 - +0.6 2,999,142 - +0.7 2,999,142 - -0.3 2,999,142 - -1.2 2,999,146 - -0.9 2,999,146 - -0.9 2,999,146 - -0.7 2,999,146 - -1.0 2,999,146 - +1.1 2,999,146 - -2.2 2,999,148 - -0.0 2,999,148 - -2.4 2,999,148 - +0.6 2,999,148 - +0.1 2,999,148 - +0.7 2,999,148 - +1.5 2,999,148 - -0.3 2,999,148 - +0.4 2,999,148 - -1.4 2,999,148 - -0.1 2,999,148 - +0.1 2,999,148 - +0.4 2,999,148 - -1.0 2,999,148 - -1.0 3,000,368 - +1.7 3,000,409 + +0.1 3,000,409 + -0.2 3,000,409 + +0.1 3,000,410 - -0.9 3,000,410 - -1.9 3,000,410 - +0.9 3,000,410 - +0.6 3,000,410 - +0.3 3,000,410 - +1.7 3,000,410 - -0.9 3,000,420 + -1.2 3,000,619 + -0.4 3,000,620 - +1.1 3,000,761 - Pf1N1B4_2664 0.12 -0.4 3,000,822 + Pf1N1B4_2664 0.18 -0.9 3,000,923 + Pf1N1B4_2664 0.27 -0.3 3,000,923 + Pf1N1B4_2664 0.27 -1.4 3,000,923 + Pf1N1B4_2664 0.27 -2.7 3,000,923 + Pf1N1B4_2664 0.27 +0.1 3,000,923 + Pf1N1B4_2664 0.27 +0.1 3,000,974 + +0.1 3,000,974 + Pf1N1B4_2664 0.31 +0.0 3,000,975 - -0.4 3,001,046 - Pf1N1B4_2664 0.37 +2.1 3,001,046 - -0.9 3,001,083 + Pf1N1B4_2664 0.41 +0.9
Or see this region's nucleotide sequence