Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2044 and Pf1N1B4_2045 are separated by 152 nucleotides Pf1N1B4_2045 and Pf1N1B4_2046 are separated by 159 nucleotides Pf1N1B4_2046 and Pf1N1B4_2047 overlap by 1 nucleotides Pf1N1B4_2047 and Pf1N1B4_2048 are separated by 186 nucleotides Pf1N1B4_2048 and Pf1N1B4_2049 overlap by 4 nucleotides Pf1N1B4_2049 and Pf1N1B4_2050 are separated by 19 nucleotides
Pf1N1B4_2044 - Phosphate regulon transcriptional regulatory protein PhoB (SphR), at 2,333,182 to 2,333,871
_2044
Pf1N1B4_2045 - Orf21; putative lipoprotein, at 2,334,024 to 2,334,401
_2045
Pf1N1B4_2046 - 4-hydroxybenzoate polyprenyltransferase (EC 2.5.1.39), at 2,334,561 to 2,335,451
_2046
Pf1N1B4_2047 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 2,335,451 to 2,335,885
_2047
Pf1N1B4_2048 - hypothetical protein, at 2,336,072 to 2,336,218
_2048
Pf1N1B4_2049 - Rubredoxin, at 2,336,215 to 2,336,382
_2049
Pf1N1B4_2050 - Rubredoxin-NAD(+) reductase (EC 1.18.1.1), at 2,336,402 to 2,337,550
_2050
Position (kb)
2334
2335
2336 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.564 kb on + strand, within Pf1N1B4_2044 at 2333.565 kb on - strand, within Pf1N1B4_2044 at 2333.565 kb on - strand, within Pf1N1B4_2044 at 2333.565 kb on - strand, within Pf1N1B4_2044 at 2333.565 kb on - strand, within Pf1N1B4_2044 at 2333.565 kb on - strand, within Pf1N1B4_2044 at 2333.565 kb on - strand, within Pf1N1B4_2044 at 2333.790 kb on - strand, within Pf1N1B4_2044 at 2333.892 kb on + strand at 2333.892 kb on + strand at 2333.892 kb on + strand at 2333.892 kb on + strand at 2333.892 kb on + strand at 2333.892 kb on + strand at 2333.892 kb on + strand at 2333.892 kb on + strand at 2333.893 kb on - strand at 2333.893 kb on - strand at 2333.893 kb on - strand at 2333.893 kb on - strand at 2333.893 kb on - strand at 2333.911 kb on + strand at 2333.911 kb on + strand at 2333.911 kb on + strand at 2333.911 kb on + strand at 2333.911 kb on + strand at 2333.912 kb on - strand at 2333.912 kb on - strand at 2333.912 kb on - strand at 2333.924 kb on + strand at 2333.924 kb on + strand at 2333.924 kb on + strand at 2333.925 kb on - strand at 2333.943 kb on + strand at 2333.943 kb on + strand at 2333.943 kb on + strand at 2333.943 kb on + strand at 2333.943 kb on + strand at 2333.944 kb on - strand at 2333.979 kb on + strand at 2333.979 kb on + strand at 2333.979 kb on + strand at 2333.979 kb on + strand at 2333.980 kb on - strand at 2333.980 kb on - strand at 2333.980 kb on - strand at 2333.980 kb on - strand at 2333.980 kb on - strand at 2333.980 kb on - strand at 2333.980 kb on - strand at 2333.980 kb on - strand at 2333.986 kb on + strand at 2333.986 kb on + strand at 2333.986 kb on + strand at 2333.986 kb on + strand at 2333.986 kb on + strand at 2333.986 kb on + strand at 2333.987 kb on - strand at 2333.987 kb on - strand at 2333.987 kb on - strand at 2334.147 kb on - strand, within Pf1N1B4_2045 at 2334.147 kb on - strand, within Pf1N1B4_2045 at 2334.147 kb on - strand, within Pf1N1B4_2045 at 2334.147 kb on - strand at 2334.156 kb on + strand, within Pf1N1B4_2045 at 2334.156 kb on + strand, within Pf1N1B4_2045 at 2334.156 kb on + strand, within Pf1N1B4_2045 at 2334.157 kb on + strand, within Pf1N1B4_2045 at 2334.157 kb on - strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.158 kb on + strand, within Pf1N1B4_2045 at 2334.159 kb on - strand, within Pf1N1B4_2045 at 2334.159 kb on - strand, within Pf1N1B4_2045 at 2334.159 kb on - strand, within Pf1N1B4_2045 at 2334.159 kb on - strand, within Pf1N1B4_2045 at 2334.159 kb on - strand, within Pf1N1B4_2045 at 2334.159 kb on - strand, within Pf1N1B4_2045 at 2334.159 kb on - strand, within Pf1N1B4_2045 at 2334.303 kb on + strand, within Pf1N1B4_2045 at 2334.303 kb on + strand, within Pf1N1B4_2045 at 2334.303 kb on + strand, within Pf1N1B4_2045 at 2334.303 kb on + strand, within Pf1N1B4_2045 at 2334.304 kb on - strand, within Pf1N1B4_2045 at 2334.304 kb on - strand, within Pf1N1B4_2045 at 2334.304 kb on - strand, within Pf1N1B4_2045 at 2334.305 kb on + strand, within Pf1N1B4_2045 at 2334.305 kb on + strand, within Pf1N1B4_2045 at 2334.305 kb on + strand, within Pf1N1B4_2045 at 2334.305 kb on + strand, within Pf1N1B4_2045 at 2334.305 kb on + strand, within Pf1N1B4_2045 at 2334.306 kb on - strand, within Pf1N1B4_2045 at 2334.306 kb on - strand, within Pf1N1B4_2045 at 2334.306 kb on - strand, within Pf1N1B4_2045 at 2334.306 kb on - strand, within Pf1N1B4_2045 at 2334.321 kb on + strand, within Pf1N1B4_2045 at 2334.321 kb on + strand, within Pf1N1B4_2045 at 2334.321 kb on + strand, within Pf1N1B4_2045 at 2334.321 kb on + strand, within Pf1N1B4_2045 at 2334.321 kb on + strand, within Pf1N1B4_2045 at 2334.321 kb on + strand, within Pf1N1B4_2045 at 2334.321 kb on + strand, within Pf1N1B4_2045 at 2334.322 kb on - strand, within Pf1N1B4_2045 at 2334.322 kb on - strand, within Pf1N1B4_2045 at 2334.322 kb on - strand, within Pf1N1B4_2045 at 2334.322 kb on - strand, within Pf1N1B4_2045 at 2334.322 kb on - strand, within Pf1N1B4_2045 at 2334.322 kb on - strand at 2334.322 kb on - strand, within Pf1N1B4_2045 at 2334.322 kb on - strand, within Pf1N1B4_2045 at 2334.322 kb on - strand, within Pf1N1B4_2045 at 2334.322 kb on - strand, within Pf1N1B4_2045 at 2334.368 kb on + strand at 2334.368 kb on + strand at 2334.390 kb on + strand at 2334.391 kb on - strand at 2334.484 kb on + strand at 2334.491 kb on + strand at 2334.574 kb on + strand at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.768 kb on - strand, within Pf1N1B4_2047 at 2335.768 kb on - strand at 2335.922 kb on + strand at 2335.922 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.937 kb on - strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.053 kb on - strand at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.208 kb on + strand at 2336.209 kb on - strand at 2336.209 kb on - strand at 2336.251 kb on + strand, within Pf1N1B4_2049 at 2336.251 kb on + strand, within Pf1N1B4_2049 at 2336.252 kb on - strand at 2336.252 kb on - strand, within Pf1N1B4_2049 at 2336.252 kb on - strand at 2336.252 kb on - strand, within Pf1N1B4_2049 at 2336.380 kb on + strand at 2336.380 kb on + strand at 2336.380 kb on + strand at 2336.381 kb on - strand at 2336.381 kb on - strand at 2336.381 kb on - strand at 2336.381 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,333,564 + Pf1N1B4_2044 0.55 -0.5 2,333,564 + Pf1N1B4_2044 0.55 -1.1 2,333,564 + Pf1N1B4_2044 0.55 +0.0 2,333,564 + Pf1N1B4_2044 0.55 +2.1 2,333,564 + Pf1N1B4_2044 0.55 -0.7 2,333,564 + Pf1N1B4_2044 0.55 +1.9 2,333,564 + Pf1N1B4_2044 0.55 -1.0 2,333,564 + Pf1N1B4_2044 0.55 -2.3 2,333,564 + Pf1N1B4_2044 0.55 -0.5 2,333,564 + Pf1N1B4_2044 0.55 -0.6 2,333,564 + Pf1N1B4_2044 0.55 +0.3 2,333,565 - Pf1N1B4_2044 0.56 +1.3 2,333,565 - Pf1N1B4_2044 0.56 +0.3 2,333,565 - Pf1N1B4_2044 0.56 -0.1 2,333,565 - Pf1N1B4_2044 0.56 -0.9 2,333,565 - Pf1N1B4_2044 0.56 +1.0 2,333,565 - Pf1N1B4_2044 0.56 -0.5 2,333,790 - Pf1N1B4_2044 0.88 -0.7 2,333,892 + -0.2 2,333,892 + +0.0 2,333,892 + -0.7 2,333,892 + -2.7 2,333,892 + +1.0 2,333,892 + -1.5 2,333,892 + +0.7 2,333,892 + -2.5 2,333,893 - -0.1 2,333,893 - +0.3 2,333,893 - -0.3 2,333,893 - -1.4 2,333,893 - -1.3 2,333,911 + +0.5 2,333,911 + +1.1 2,333,911 + -1.1 2,333,911 + +1.3 2,333,911 + +0.6 2,333,912 - +0.3 2,333,912 - +0.8 2,333,912 - +1.4 2,333,924 + -0.5 2,333,924 + -0.5 2,333,924 + -2.5 2,333,925 - +0.3 2,333,943 + -1.3 2,333,943 + +0.7 2,333,943 + -1.3 2,333,943 + -1.3 2,333,943 + +1.3 2,333,944 - +1.2 2,333,979 + +0.5 2,333,979 + -0.3 2,333,979 + -1.7 2,333,979 + +1.0 2,333,980 - +0.3 2,333,980 - -1.3 2,333,980 - +0.3 2,333,980 - -0.3 2,333,980 - +0.5 2,333,980 - -0.3 2,333,980 - +0.3 2,333,980 - +0.3 2,333,986 + +0.4 2,333,986 + -0.7 2,333,986 + -0.4 2,333,986 + +0.5 2,333,986 + -1.4 2,333,986 + -0.5 2,333,987 - -0.3 2,333,987 - -0.2 2,333,987 - +0.3 2,334,147 - Pf1N1B4_2045 0.33 -0.9 2,334,147 - Pf1N1B4_2045 0.33 -2.4 2,334,147 - Pf1N1B4_2045 0.33 +0.2 2,334,147 - -1.3 2,334,156 + Pf1N1B4_2045 0.35 -1.3 2,334,156 + Pf1N1B4_2045 0.35 -1.7 2,334,156 + Pf1N1B4_2045 0.35 +0.6 2,334,157 + Pf1N1B4_2045 0.35 +0.1 2,334,157 - Pf1N1B4_2045 0.35 +0.3 2,334,158 + Pf1N1B4_2045 0.35 +0.6 2,334,158 + Pf1N1B4_2045 0.35 -0.7 2,334,158 + Pf1N1B4_2045 0.35 -1.1 2,334,158 + Pf1N1B4_2045 0.35 -0.2 2,334,158 + Pf1N1B4_2045 0.35 -0.5 2,334,158 + Pf1N1B4_2045 0.35 -1.1 2,334,158 + Pf1N1B4_2045 0.35 +1.3 2,334,158 + Pf1N1B4_2045 0.35 -0.4 2,334,158 + Pf1N1B4_2045 0.35 -1.3 2,334,158 + Pf1N1B4_2045 0.35 -1.1 2,334,158 + Pf1N1B4_2045 0.35 -1.0 2,334,159 - Pf1N1B4_2045 0.36 +0.3 2,334,159 - Pf1N1B4_2045 0.36 -2.1 2,334,159 - Pf1N1B4_2045 0.36 -2.1 2,334,159 - Pf1N1B4_2045 0.36 -0.9 2,334,159 - Pf1N1B4_2045 0.36 -3.1 2,334,159 - Pf1N1B4_2045 0.36 -0.3 2,334,159 - Pf1N1B4_2045 0.36 +1.1 2,334,303 + Pf1N1B4_2045 0.74 -0.1 2,334,303 + Pf1N1B4_2045 0.74 +1.3 2,334,303 + Pf1N1B4_2045 0.74 -0.3 2,334,303 + Pf1N1B4_2045 0.74 -0.3 2,334,304 - Pf1N1B4_2045 0.74 -1.1 2,334,304 - Pf1N1B4_2045 0.74 +1.3 2,334,304 - Pf1N1B4_2045 0.74 +0.7 2,334,305 + Pf1N1B4_2045 0.74 -0.7 2,334,305 + Pf1N1B4_2045 0.74 -0.7 2,334,305 + Pf1N1B4_2045 0.74 -0.4 2,334,305 + Pf1N1B4_2045 0.74 -0.0 2,334,305 + Pf1N1B4_2045 0.74 -0.4 2,334,306 - Pf1N1B4_2045 0.75 -0.5 2,334,306 - Pf1N1B4_2045 0.75 -0.2 2,334,306 - Pf1N1B4_2045 0.75 -1.3 2,334,306 - Pf1N1B4_2045 0.75 +1.6 2,334,321 + Pf1N1B4_2045 0.79 +1.3 2,334,321 + Pf1N1B4_2045 0.79 +0.6 2,334,321 + Pf1N1B4_2045 0.79 +2.6 2,334,321 + Pf1N1B4_2045 0.79 -0.1 2,334,321 + Pf1N1B4_2045 0.79 -1.7 2,334,321 + Pf1N1B4_2045 0.79 -1.0 2,334,321 + Pf1N1B4_2045 0.79 -0.7 2,334,322 - Pf1N1B4_2045 0.79 -0.7 2,334,322 - Pf1N1B4_2045 0.79 -0.9 2,334,322 - Pf1N1B4_2045 0.79 +0.9 2,334,322 - Pf1N1B4_2045 0.79 +0.3 2,334,322 - Pf1N1B4_2045 0.79 -1.0 2,334,322 - +2.3 2,334,322 - Pf1N1B4_2045 0.79 -1.3 2,334,322 - Pf1N1B4_2045 0.79 +0.3 2,334,322 - Pf1N1B4_2045 0.79 +0.7 2,334,322 - Pf1N1B4_2045 0.79 -1.3 2,334,368 + -0.1 2,334,368 + -0.1 2,334,390 + -0.5 2,334,391 - -1.1 2,334,484 + +0.9 2,334,491 + -0.6 2,334,574 + -0.7 2,335,687 + Pf1N1B4_2047 0.54 +0.3 2,335,687 + Pf1N1B4_2047 0.54 +0.9 2,335,687 + Pf1N1B4_2047 0.54 -0.4 2,335,687 + Pf1N1B4_2047 0.54 -0.1 2,335,687 + Pf1N1B4_2047 0.54 +0.9 2,335,767 + Pf1N1B4_2047 0.73 +0.9 2,335,767 + Pf1N1B4_2047 0.73 +0.3 2,335,767 + Pf1N1B4_2047 0.73 +1.3 2,335,767 + Pf1N1B4_2047 0.73 +0.3 2,335,767 + Pf1N1B4_2047 0.73 -0.5 2,335,768 - Pf1N1B4_2047 0.73 -1.4 2,335,768 - -0.7 2,335,922 + +0.6 2,335,922 + -0.1 2,335,936 + -0.7 2,335,936 + -0.3 2,335,936 + +0.3 2,335,936 + -2.1 2,335,936 + -0.7 2,335,937 - -0.2 2,336,052 + -0.5 2,336,052 + +1.3 2,336,052 + -0.3 2,336,052 + -0.3 2,336,052 + +0.4 2,336,052 + -0.2 2,336,053 - -0.3 2,336,130 + Pf1N1B4_2048 0.39 -1.3 2,336,130 + Pf1N1B4_2048 0.39 -0.1 2,336,130 + Pf1N1B4_2048 0.39 +0.3 2,336,130 + Pf1N1B4_2048 0.39 -0.7 2,336,208 + -1.3 2,336,209 - -0.7 2,336,209 - +0.3 2,336,251 + Pf1N1B4_2049 0.21 -1.3 2,336,251 + Pf1N1B4_2049 0.21 -1.1 2,336,252 - +0.3 2,336,252 - Pf1N1B4_2049 0.22 -2.5 2,336,252 - -0.7 2,336,252 - Pf1N1B4_2049 0.22 -0.5 2,336,380 + -0.7 2,336,380 + +0.1 2,336,380 + -1.0 2,336,381 - -0.3 2,336,381 - +2.6 2,336,381 - -0.3 2,336,381 - -1.1
Or see this region's nucleotide sequence